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Organelle Genome Analysis in Somatic Hybrids Between Solanum tuberosum and S. pinnatisectum Revealed Diverse Cytoplasm Type in Potato

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Abstract

Organelle genome diversity was analysed in interspecific potato somatic hybrids using chloroplast (cp)- and mitochondrial (mt)-specific molecular markers. Out of total 25 markers (15 cpDNA and 10 mtDNA) tested in total 16 samples, only four mtDNA primers (rpS14/cob, Nsm2, ALM4/ALM5 and ALM6/ALM7) detected polymorphism, whereas other primers were monomorphic. Cluster analysis showed higher genetic diversity among the genotypes by mtDNA profiles than that by cpDNA. Ten haplotypes were grouped by cluster analysis comprised of maximum seven genotypes in haplotype no. 3. Monomorphic markers did not reveal variability in our samples and suggest highly conserved organelle genomic regions. New genomic arrangements were observed in the somatic hybrids for mt polymorphic loci. Our study suggests that somatic hybrids are comprised of diverse cytoplasm types consisting predominantly of T-, W-, and C-, with a few A- and S-type chloroplast, and α-, β- and γ-type mitochondrial genome, and have unique potential to widen the cultivated potato gene pool by breeding methods.

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Acknowledgments

The authors are grateful to the ICAR-Central Potato Research Institute, Shimla, and the DBT, Government of India (BT/PR4291/AGR/2/838/2011), for providing financial supports and necessary facilities. Hearty thanks are extended to Mr. Seeshram Thakur for in vitro maintenance of somatic hybrids plants.

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Authors and Affiliations

  1. Central Potato Research Institute, Shimla, Himachal Pradesh, 171 001, India

    Jagesh Kumar Tiwari, Sapna Devi, Poonam Chandel, Nilofer Ali, Vinay Bhardwaj & Bir Pal Singh

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  1. Jagesh Kumar Tiwari
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  2. Sapna Devi
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  3. Poonam Chandel
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  4. Nilofer Ali
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  5. Vinay Bhardwaj
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  6. Bir Pal Singh
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Correspondence to Jagesh Kumar Tiwari.

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Authors contribution

J.K. Tiwari, S. Devi, P. Chandel and N. Ali have performed the original research work. J.K. Tiwari has planned the work, analysed the data and prepared the manuscript. V. Bhardwaj and B.P. Singh have contributed in language correction of the manuscript.

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Tiwari, J.K., Devi, S., Chandel, P. et al. Organelle Genome Analysis in Somatic Hybrids Between Solanum tuberosum and S. pinnatisectum Revealed Diverse Cytoplasm Type in Potato. Agric Res 5, 22–28 (2016). https://doi.org/10.1007/s40003-015-0197-z

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  • DOI: https://doi.org/10.1007/s40003-015-0197-z

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