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Enhanced Bone Formation by Rapidly Formed Bony Wall over the Bone Defect Using Dual Growth Factors

Tissue Engineering and Regenerative Medicine volume 20, pages 767–778 (2023)Cite this article

Abstract

Background:

In guided bone regeneration (GBR), there are various problems that occur in the bone defect after the wound healing period. This study aimed to investigate the enhancement of the osteogenic ability of the dual scaffold complex and identify the appropriate concentration of growth factors (GF) for new bone formation based on the novel GBR concept that is applying rapid bone forming GFs to the membrane outside of the bone defect.

Methods:

Four bone defects with a diameter of 8 mm were formed in the calvaria of New Zealand white rabbits each to perform GBR. Collagen membrane and biphasic calcium phosphate (BCP) were applied to the bone defects with the four different concetration of BMP-2 or FGF-2. After 2, 4, and 8 weeks of healing, histological, histomorphometric, and immunohistochemical analyses were conducted.

Results:

In the histological analysis, continuous forms of new bones were observed in the upper part of bone defect in the experimental groups, whereas no continuous forms were observed in the control group. In the histomorphometry, The group to which BMP-2 0.5 mg/ml and FGF-2 1.0 mg/ml was applied showed statistically significantly higher new bone formation. Also, the new bone formation according to the healing period was statistically significantly higher at 8 weeks than at 2, 4 weeks.

Conclusion:

The novel GBR method in which BMP-2, newly proposed in this study, is applied to the membrane is effective for bone regeneration. In addition, the dual scaffold complex is quantitatively and qualitatively advantageous for bone regeneration and bone maintenance over time.

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Acknowledgements

We would like to acknowledge the help from Dr. Hyunmin Choi for the help with animal experiments. This research was funded by Yonsei University School of Dentistry Intramural Faculty Research Grant (Y110523) to Y.P.

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Author notes

  1. Jaehan Park and Narae Jung have contributed equally to this work as co-first authors.

Authors and Affiliations

  1. Department of Prosthodontics, Yonsei University College of Dentistry, 50-1 Yonsei-Ro, Seodaemun-Gu, Dental Hospital Room 717, Seoul, 03722, Republic of Korea

    Jaehan Park, Narae Jung, Jong-Eun Kim, Hong Seok Moon & Young-Bum Park

  2. Department of Clinical Dentistry, BK21 FOUR Project, Oral Science Research Center, Yonsei University College of Dentistry, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea

    Narae Jung

  3. Division in Anatomy and Developmental Biology, Department of Oral Biology, Taste Research Center, Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea

    Dong-Joon Lee

  4. Department of Dental Biomaterials and Institute of Biomaterials and Implant, College of Dentistry, Wonkwang University, Iksan, 54538, Republic of Korea

    Seunghan Oh

  5. Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry, 101 Daehak-Ro, Jongno-Gu, Seoul, 03080, Republic of Korea

    Sungtae Kim

  6. Division of Anatomy and Developmental Biology, Department of Oral Biology, Yonsei University College of Dentistry, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea

    Sung-Won Cho

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  1. Jaehan Park
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Correspondence to Young-Bum Park.

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Ethical Statement

The breeding, management, and surgical procedures followed the animal testing standards of the Yonsei University Health System Institutional Animal Care and Use Committee, Seoul, Korea. The animal study plan for these experiments (2016–0062) was reviewed and approved by this committee.

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Park, J., Jung, N., Lee, DJ. et al. Enhanced Bone Formation by Rapidly Formed Bony Wall over the Bone Defect Using Dual Growth Factors. Tissue Eng Regen Med 20, 767–778 (2023). https://doi.org/10.1007/s13770-023-00534-z

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  • DOI: https://doi.org/10.1007/s13770-023-00534-z

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