We aimed to validate a pilot study of photodiagnosis using near infrared (NIR) transillumination and assess the clinical efficacy of hypericin-mediated photodynamic therapy (HYP-PDT) in a rabbit laryngeal cancer model in order to develop a novel therapeutic modality with complete remission and preservation of the functional organ.
(1) In vitro study: VX tumor cells were subcultured and subjected to HYP-PDT. (2) In vivo study: A laryngeal cancer model was developed in which 12 rabbits were inoculated with a VX tumor suspension in the submucosal area of the left vocal fold using a transoral approach. All rabbits underwent NIR transillumination using light with a wavelength of 780 nm. The survival periods of the three treatment groups (6 rabbits in Group A: HYP-PDT, 3 each in Groups B and C: laser irradiation or HYP administration only) were analyzed.
The higher the HYP concentration, the lower the VX cell viability in response to HYP-PDT using 590 nm LED. Following HYP-PDT, small tumors in Group A-1 rabbits healed completely and the animals demonstrated a long survival period, and larger tumors in Group A-2 healed partially with a survival period that extended over 3 weeks after inoculation. The survival of Groups B and C were not different over the first 3 weeks of the study, and were shorter than in Group A.
We found HYP-PDT could be a curative therapy for early-stage cancers that may also preserve organ function, and may inhibit tumor progression and metastasis during advanced stages of laryngeal cancer.
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This study was supported by a grant from the National Research Foundation of Korea (NRF-2019M3E5D1A02070860).
Conflict of interest
The authors declare that they have no conflict of interest.
The animal studies were performed after receiving approval of the Institutional Animal Care and Use Committee (IACUS) at Kosin University College of Medicine (IACUC approval No. KMAP 15-07).
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Lee, H., Kim, S.W., Kwon, D.Y. et al. Near-infrared Transillumination and Photodynamic Therapy Using Hypericin in Animal Laryngeal Tumors. Tissue Eng Regen Med (2021). https://doi.org/10.1007/s13770-021-00377-6
- Photodynamic therapy
- Laryngeal cancer