Renal dysfunction remains a global issue, with chronic kidney disease being the 18th most leading cause of death, worldwide. The increased demands in kidney transplants, led the scientific society to seek alternative strategies, utilizing mostly the tissue engineering approaches. Unlike to perfusion decellularization of kidneys, we proposed alternative decellularization strategies to obtain acellular kidney scaffolds. The aim of this study was the evaluation of two different decellularization approaches for producing kidney bioscaffolds.
Rat kidneys from Wistar rats, were submitted to decellularization, followed two different strategies. The decellularization solutions used in both approaches were the same and involved the use of 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate and sodium dodecyl sulfate buffers for 12 h each, followed by incubation in a serum medium. Both approaches involved 3 decellularization cycles. Histological analysis, biochemical and DNA quantification were performed. Cytotoxicity assay and repopulation of acellular kidneys were also applied.
Histological, biochemical and DNA quantification confirmed that the 2nd approach had the best outcome regarding the kidney composition and cell elimination. Acellular kidneys from both approaches were successfully recellularized.
Based on the above data, the production of kidney scaffolds with the proposed cost- effective decellularization approaches, was efficient.
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Conflict of interest
The authors declare no conflict of interest for this study.
The study protocol involved the harvest of kidneys from Wistar Rats, weighing 300–350 gr. All care and handling of the animals were provided according to the Guide for the Care and Use of Laboratory Animals of BRFAA, conformed to the Directive 2010/63/EU of the European Parliament, and has been approved by the Bioethics Committee of BRFAA (ref 25–2019). The WJ-MSCs used in the current research study were provided by the Hellenic Cord Blood Bank (HCBB) located at the Biomedical Research Foundation Academy of Athens (BRFAA). All human umbilical cords, used for the WJ-MSCs, were accompanied by informed consent and signed by the mothers before the delivery. In addition all human umbilical cords were derived from male donors. Also, the informed consent was in accordance with the ethical standards of the Greek National Ethical Committee and has been accepted by the Institution’s ethical board (Ref 2578/32).
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Mallis, P., Oikonomidis, C., Dimou, Z. et al. Optimizing Decellularization Strategies for the Efficient Production of Whole Rat Kidney Scaffolds. Tissue Eng Regen Med 18, 623–640 (2021). https://doi.org/10.1007/s13770-021-00339-y
- Kidney scaffold
- Chronic kidney disease
- Mesenchymal stromal cells
- Static seeding