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Hybrid Additive Microfabrication Scaffold Incorporated with Highly Aligned Nanofibers for Musculoskeletal Tissues

  • Dilshan Sooriyaarachchi
  • Hugo J. Minière
  • Shahrima Maharubin
  • George Z. Tan
Original Article
  • 19 Downloads

Abstract

Background:

Latest tissue engineering strategies for musculoskeletal tissues regeneration focus on creating a biomimetic microenvironment closely resembling the natural topology of extracellular matrix. This paper presents a novel musculoskeletal tissue scaffold fabricated by hybrid additive manufacturing method.

Methods:

The skeleton of the scaffold was 3D printed by fused deposition modeling, and a layer of random or aligned polycaprolactone nanofibers were embedded between two frames. A parametric study was performed to investigate the effects of process parameters on nanofiber morphology. A compression test was performed to study the mechanical properties of the scaffold. Human fibroblast cells were cultured in the scaffold for 7 days to evaluate the effect of scaffold microstructure on cell growth.

Results:

The tip-to-collector distance showed a positive correlation with the fiber alignment, and the electrospinning time showed a negative correlation with the fiber density. With reinforced nanofibers, the hybrid scaffold demonstrated superior compression strength compared to conventional 3D-printed scaffold. The hybrid scaffold with aligned nanofibers led to higher cell attachment and proliferation rates, and a directional cell organization. In addition, there was a nonlinear relationship between the fiber diameter/density and the cell actinfilament density.

Conclusion:

This hybrid biofabrication process can be established as a highly efficient and scalable platform to fabricate biomimetic scaffolds with patterned fibrous microstructure, and will facilitate future development of clinical solutions for musculoskeletal tissue regeneration.

Keywords

Musculoskeletal tissues Hybrid biofabrication Patterned fibrous microstructure 3D printing Electrospinning 

Notes

Acknowledgements

This paper was financially supported by the Foundation of the Whitacre College of Engineering and the Office of Vice President for Research at Texas Tech University.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Ethical statement

The protocol of cell culture study was approved by the Texas Tech University Institutional Biosafety Committee (IBC#: 1705B1).

References

  1. 1.
    Grindem H, Snyder-Mackler L, Moksnes H, Engebretsen L, Risberg MA. Simple decision rules can reduce reinjury risk by 84% after ACL reconstruction: the Delaware–Oslo ACL cohort study. Br J Sports Med. 2016;50:804–8.CrossRefGoogle Scholar
  2. 2.
    Colvin AC, Egorova N, Harrison AK, Moskowitz A, Flatow EL. National trends in rotator cuff repair. J Bone Joint Surg Am. 2012;94:227–33.CrossRefGoogle Scholar
  3. 3.
    Smith L, Xia Y, Galatz LM, Genin GM, Thomopoulos S. Tissue-engineering strategies for the tendon/ligament-to-bone insertion. Connect Tissue Res. 2012;53:95–105.CrossRefGoogle Scholar
  4. 4.
    Mather RC 3rd, Koenig L, Kocher MS, Dall TM, Gallo P, Scott DJ, et al. Societal and economic impact of anterior cruciate ligament tears. J Bone Joint Surg Am. 2013;95:1751–9.CrossRefGoogle Scholar
  5. 5.
    Howell R, Kumar NS, Patel N, Tom J. Degenerative meniscus: pathogenesis, diagnosis, and treatment options. World J Orthop. 2014;5:597–602.CrossRefGoogle Scholar
  6. 6.
    Tseng Q, Duchemin-Pelletier E, Deshiere A, Balland M, Guillou H, Filhol O, et al. Spatial organization of the extracellular matrix regulates cell–cell junction positioning. Proc Natl Acad Sci U S A. 2012;109:1506–11.CrossRefGoogle Scholar
  7. 7.
    Geckil H, Xu F, Zhang X, Moon S, Demirci U. Engineering hydrogels as extracellular matrix mimics. Nanomedicine (Lond). 2010;5:469–84.CrossRefGoogle Scholar
  8. 8.
    Shim JH, Kim JY, Park M, Park J, Cho DW. Development of a hybrid scaffold with synthetic biomaterials and hydrogel using solid freeform fabrication technology. Biofabrication. 2011;3:034102.CrossRefGoogle Scholar
  9. 9.
    Yan F, Liu Y, Chen H, Zhang F, Zheng L, Hu Q. A multi-scale controlled tissue engineering scaffold prepared by 3D printing and NFES technology. AIP Adv. 2014;4:031321.CrossRefGoogle Scholar
  10. 10.
    Benjamin M, Ralphs JR. Fibrocartilage in tendons and ligaments—an adaptation to compressive load. J Anat. 1998;193:481–94.CrossRefGoogle Scholar
  11. 11.
    Augst A, Marolt D, Freed LE, Vepari C, Meinel L, Farley M, et al. Effects of chondrogenic and osteogenic regulatory factors on composite constructs grown using human mesenchymal stem cells, silk scaffolds and bioreactors. J R Soc Interface. 2008;5:929–39.CrossRefGoogle Scholar
  12. 12.
    Law JX, Liau LL, Saim A, Yang Y, Idrus R. Electrospun collagen nanofibers and their applications in skin tissue engineering. Tissue Eng Regen Med. 2017;14:699–718.CrossRefGoogle Scholar
  13. 13.
    Burton TP, Callanan A. A non-woven path: electrospun poly (lactic acid) scaffolds for kidney tissue engineering. Tissue Eng Regen Med. 2018;15:301–10.CrossRefGoogle Scholar
  14. 14.
    Xu T, Binder KW, Albanna MZ, Dice D, Zhao W, Yoo JJ, et al. Hybrid printing of mechanically and biologically improved constructs for cartilage tissue engineering applications. Biofabrication. 2013;5:015001.CrossRefGoogle Scholar
  15. 15.
    Lee SJ, Heo DN, Park JS, Kwon SK, Lee JH, Lee JH, et al. Characterization and preparation of bio-tubular scaffolds for fabricating artificial vascular grafts by combining electrospinning and a 3D printing system. Phys Chem Chem Phys. 2015;17:2996–9.CrossRefGoogle Scholar
  16. 16.
    Williams A, Nowak JF, Dass R, Samuel J, Mills KL. Toward morphologically relevant extracellular matrix in vitro models: 3D fiber reinforced hydrogels. Front Physiol. 2018;9:966.CrossRefGoogle Scholar
  17. 17.
    Baek J, Sovani S, Choi W, Jin S, Grogan SP, D’Lima DD. Meniscal tissue engineering using aligned collagen fibrous scaffolds: comparison of different human cell sources. Tissue Eng Part A. 2018;24:81–93.CrossRefGoogle Scholar
  18. 18.
    Bhardwaj N, Kundu SC. Electrospinning: a fascinating fiber fabrication technique. Biotechnol Adv. 2010;28:325–47.CrossRefGoogle Scholar
  19. 19.
    Liu W, Li Z, Zheng L, Zhang X, Liu P, Yang T, et al. Electrospun fibrous silk fibroin/poly(L-lactic acid) scaffold for cartilage tissue engineering. Tissue Eng Regen Med. 2016;13:516–26.CrossRefGoogle Scholar
  20. 20.
    Choi JS, Lee SJ, Christ GJ, Atala A, Yoo JJ. The influence of electrospun aligned poly (ɛ-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubes. Biomaterials. 2008;29:2899–906.CrossRefGoogle Scholar

Copyright information

© The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Industrial, Manufacturing and Systems EngineeringTexas Tech UniversityLubbockUSA

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