Tissue Engineering and Regenerative Medicine

, Volume 15, Issue 4, pp 477–492 | Cite as

Mesenchymal Stem Cells of Different Origin-Seeded Bioceramic Construct in Regeneration of Bone Defect in Rabbit

  • Swapan Kumar MaitiEmail author
  • M. U. Shivakumar
  • Divya Mohan
  • Naveen Kumar
  • Karam Pal Singh
Original Article



Stem cell is currently playing a major role in the treatment of number of incurable diseases via transplantation therapy. The objective of this study was to determine the osteogenic potential of allogenic and xenogenic bone-derived MSC seeded on a hydroxyapatite (HA/TCP) bioceramic construct in critical size bone defect (CSD) in rabbits.


A 15 mm long radial osteotomy was performed unilaterally in thirty-six rabbits divided equally in six groups. Bone defects were filled with bioscaffold seeded with autologous, allogenic, ovine, canine BMSCs and cell free bioscaffold block in groups A, B, C, D and E respectively. An empty defect served as the control group.


The radiological, histological and SEM observations depicted better and early signs of new bone formation and bridging bone/implant interfaces in the animals of group A followed by B. Both xenogenous MSC-HA/TCP construct also accelerated the healing of critical sized bone defect. There was no sign of any inflammatory reaction in the xenogenic composite scaffold group of animals confirmed their well acceptance by the host body.


In vivo experiments in rabbit CSD model confirmed that autogenous, allogenous and xenogenous BMSC seeded on bioscaffold promoted faster healing of critical size defects. Hence, we may suggest that BMSCs are suitable for bone formation in fracture healing and non-union.


Mesenchymal stem cells Hydroxyapatite bioceramic Allogenic Xenogenic Bone healing 



The authors wish to thank Prof. H. Varma, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala (India) for his technical assistance for designing and preparation of HA/TCP bioceramic.

Author contributions

SKM conceived and designed the study. SKM and SMU performed the experiments. KPS performed the histological analysis. SKM, SMU, KPS and NK analyzed and interpreted the data. SKM, SMU, and NK contributed to research infrastructure. SKM and DM wrote the paper. All authors have given approval to the final version of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

This study was conducted after getting approval from the ICAR-IVRI—Institute Animal Ethics Committee for Animal Care and Animal Experimentation [IAEC, No-F. 1-53/2012-13-JD (Research)].


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Copyright information

© The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Division of SurgeryICAR-Indian Veterinary Research Institute (Deemed University)IzatnagarIndia
  2. 2.Centre for Animal Disease Research and DiagnosisICAR-Indian Veterinary Research Institute (Deemed University)IzatnagarIndia

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