Tissue Engineering and Regenerative Medicine

, Volume 15, Issue 4, pp 437–444 | Cite as

A Pathophysiological Validation of Collagenase II-Induced Biochemical Osteoarthritis Animal Model in Rabbit

  • Jaeseong Park
  • Jungsun Lee
  • Kang-Il Kim
  • Jisoo Lee
  • Seoyoung Jang
  • Hyun Tae Choi
  • Youngsook Son
  • Hyung Joong Kim
  • Eung Je Woo
  • EunAh LeeEmail author
  • Tong In OhEmail author
Original Article



Current dilemma working with surgically-induced OA (osteoarthritis) model include inconsistent pathological state due to various influence from surrounding tissues. On the contrary, biochemical induction of OA using collagenase II has several advantageous points in a sense that it does not involve surgery to induce model and the extent of induced cartilage degeneration is almost uniform. However, concerns still exists because biochemical OA model induce abrupt destruction of cartilage tissues through enzymatic digestion in a short period of time, and this might accompany systemic inflammatory response, which is rather a trait of RA (rheumatoid arthritis) than being a trait of OA.


To clear the concern about the systemic inflammatory response that might be caused by abrupt destruction of cartilage tissue, OA was induced to only one leg of an animal and the other leg was examined to confirm the presence of systemic degenerative effect.


Although the cartilage tissues were rapidly degenerated during short period of time upon biochemical induction of OA, they did not accompanied with RA-like process based on the histology data showing degeneration of articular cartilage occurred only in the collagenase-injected knee joint. Scoring evaluation data indicated that the cartilage tissues in non-induced joint remained intact. Neutrophil count transiently increase between day 8 and day 16, and there were no significant change in other complete blood count profile showing a characteristics of OA disease.


These study shows that biochemically induced cartilage degeneration truly represented uniform and reliable OA state.


Animal model Osteoarthritis Cartilage Regeneration Inflammation 



This work was supported by National Research Foundation of Korea (2017R1A2A1A05001330, NRF-2015R1D1A1A01059702, and NRF-2015R1A2A2A04006172).

Authors’ contributions

JP, Collection and assembly of data, data analysis and interpretation, manuscript writing. JL, Conception and design, data analysis and interpretation, manuscript writing. K-IK, Conception and design, financial support, data analysis and interpretation. JL, Collection and assembly of data, data analysis and interpretation. SJ, Collection and assembly of data, data analysis and interpretation. HTC, Collection and assembly of data, data analysis and interpretation. YS, Conception and design, data analysis and interpretation. HJK, Conception and design, data analysis and interpretation. EJW, Conception and design, data analysis and interpretation. EL, Conception and design, financial support, collection and assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript. TIO, Conception and design, financial support, data analysis and interpretation, manuscript writing, and final approval of manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no potential conflicts of interest.

Ethical approval

All animal studies were reviewed and approved by the Institutional Animal Care and Use Committee of the Kyung Hee University (KHMC-IACUC-2017-19) and all procedures including animal care were performed according to NIH guideline.


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Copyright information

© The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Medical Engineering, Graduate SchoolKyung Hee UniversitySeoulSouth Korea
  2. 2.R&D InstituteBiosolution Inc.SeoulSouth Korea
  3. 3.Department of Orthopaedic Surgery, School of MedicineKyung Hee UniversitySeoulSouth Korea
  4. 4.Department of BiologyKyung Hee UniversitySeoulSouth Korea
  5. 5.Department of Genetic Engineering and Graduate School of BiotechnologyKyung Hee UniversityYong-inSouth Korea
  6. 6.Impedance Imaging Research CenterKyung Hee UniversitySeoulSouth Korea
  7. 7.Department of Biomedical Engineering, School of MedicineKyung Hee UniversitySeoulSouth Korea

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