Tissue Engineering and Regenerative Medicine

, Volume 15, Issue 2, pp 223–230 | Cite as

The Application of Fibrin/Hyaluronic Acid–Poly(l-Lactic-co-Glycolic Acid) Construct in Augmentation Rhinoplasty

  • Ho-Ryun Won
  • Yoo Suk Kim
  • Jong-Eun Won
  • Yoo Seob Shin
  • Chul-Ho Kim
Original Article


Although many graft materials have been used for augmentation rhinoplasty, an ideal graft has not yet been developed. As the field of tissue engineering has been developing, it has been applied to the reconstruction of many organs, but its application in the rhinoplasty field is still limited. This study evaluated the utility of allogenic chondrocytes with fibrin/hyaluronic acid (HA)–poly(l-lactic-co-glycolic acid) (PLGA) constructs in augmentation rhinoplasty. Chondrocytes from rabbit auricular cartilage were isolated and cultured with fibrin/HA hydrogels and implanted into PLGA scaffolds. After 8 weeks of in vitro culture, the scaffolds were implanted in the nasal dorsum of six rabbits. Eight weeks postoperatively, the implanted sites were evaluated with gross, radiologic, and histologic analysis. In vitro, more than 90% of the seeded chondrocytes in the PLGA scaffolds survived for 2 weeks, and they produced a large amount of extracellular matrix and were well differentiated. The grafts maintained their initial shape for 8 weeks after implantation. Radiological and histological evaluations showed that the structure was well maintained with minimal inflammatory response and appropriate elevation levels. However, the formation of neo-chondrocytes was not observed. PLGA scaffolds seeded with fibrin/HA and allogenic chondrocytes can be a biocompatible augmentation material in rhinoplasty in the future.


Rhinoplasty Augmentation materials Poly(l-lactic-co-glycolic acid) Chondrocyte Tissue engineering 



This research was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (Grant Number: HI14C2108).

Compliance with ethical standards

Conflicts of interest

The authors have declared no conflicts of interest.

Ethical statement

This study was conducted under the approval of the Institutional Animal Experiment Committee at Ajou University School of Medicine (IACUC number: 2017-0023).


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Copyright information

© The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of OtolaryngologyAjou University School of MedicineSuwonRepublic of Korea
  2. 2.Yonsei ENT ClinicSeoulRepublic of Korea
  3. 3.Department of Molecular Science and TechnologyAjou UniversitySuwonRepublic of Korea

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