Chondrogenic Potential of Dedifferentiated Rat Chondrocytes Reevaluated in Two- and Three-Dimensional Culture Conditions

Original Article
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Abstract

For the cartilage repair, the cell sources currently adopted are primarily chondrocytes or mesenchymal stem cells (MSCs). Due to the fact that chondrocytes dedifferentiate during 2-dimensional (2D) expansion, MSCs are generally more studied and considered to have higher potential for cartilage repair purposes. Here we question if the dedifferentiated chondrocytes can regain the chondrogenic potential, to find potential applications in cartilage repair. For this we chose chondrocytes at passage 12 (considered to have sufficiently dedifferentiated) and the expression of chondrogenic phenotypes and matrix syntheses were examined over 14 days. In particular, the chondrogenic potential of MSCs was also compared. Results showed that the dedifferentiated chondrocytes proliferated actively over 14 days with almost 2.5-fold increase relative to MSCs. Moreover, the chondrogenic ability of chondrocytes was significantly higher than that of MSCs, as confirmed by the expression of a series of mRNA levels and the production of cartilage extracellular matrix molecules in 2D-monolayer and 3-dimensional (3D)-spheroid cultures. Of note, the significance was higher in 3D-culture than in 2D-culture. Although more studies are needed such as the use of different cell passages and human cell source, and the chondrogenic confirmation under in vivo conditions, this study showing that the dedifferentiated chondrocytes can also be a suitable cell source for the cell-based cartilage repair, as a counterpart of MSCs, will encourage further studies regarding this issue.

Keywords

Dedifferentiated chondrocytes Mesenchymal stem cells Chondrogenesis Three-dimension 

Notes

Acknowledgement

The present research was conducted by the research fund of Dankook University in 2016.

Compliance with ethical standards

Conflict of interest

Authors (GZJ and HWK) declare no conflict of interests exists.

Ethical statement

The cell isolation from Sprague–Dawley rats was according to the consent from Dankook University Institutional Animal Care and Use Committee (DKU-IRB-2014-039).

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Copyright information

© The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  1. 1.Institute of Tissue Regeneration Engineering (ITREN)Dankook UniversityCheonanKorea
  2. 2.Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative MedicineDankook UniversityCheonanKorea
  3. 3.Department of Biomaterials Science, School of DentistryDankook UniversityCheonanKorea

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