Skip to main content
Log in

Fabrication and characterization of hydrocolloid dressing with silk fibroin nanoparticles for wound healing

  • Original Article
  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope


Hydrocolloid dressings have been developed for many types of wound healing. In particular, dressing is a critical component in the successful recover of burn injuries, which causes a great number of people to not only suffer from physical but also psychological and economic anguish each year. Additionally, silk fibroin is the safest material for tissue engineering due to biocompatibility. In this study, we fabricated hydrocolloid dressings incorporating silk fibroin nanoparticles to enhance the efficacy of hydrocolloid dressing and then use this silk fibroin nanoparticle hydrocolloid dressing (SFNHD) in animal models to treat burn wounds. The structures and properties of SFNHD were characterized using tensile strength and Cell Counting Kit-8 assay. The results indicated the structural stability and the cellular biocompatibility of the hydrocolloid dressing suggesting that SFNHD can be applied to the treatment of wounds. To demonstrate the capacity of a silk fibroin hydrocolloid dressing to treat burn wounds, we compared SFNHD to gauze and Neoderm®, a commercially available dressing. This study clearly demonstrated accelerated wound healing with greater wound structural integrity and minimal wound size after treatment with SFNHD. These observations indicate that SFNHD may be an improvement upon current standard dressings such as Gauze and Neoderm® for burn wounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others


  1. Lee JH, Lee SJ, Kim SJ, Kim KH, Kim YL, Song JE, et al. Effect of silk in Silk/PLGA hybrid films on attachment and proliferation of human aortic endothelial cells. Polymer Korea 2013;37:127–134.

    Article  CAS  Google Scholar 

  2. Mondal M, Trivedy K, Kumar SN. The silk proteins, sericin and fibroin in silkworm, Bombyx mori Linn.,-a review. Caspian J Env Sci 2007;5:63–76.

    Google Scholar 

  3. Shimura K, Kikuchi A, Ohtomo K, Katagata Y, Hyodo A. Studies on silk fibroin of Bombyx mori. I. Fractionation of fibroin prepared from the posterior silk gland. J Biochem 1976;80:693–702.

    CAS  PubMed  Google Scholar 

  4. Yilgor P, Sousa RA, Reis RL, Hasirci N, Hasirci V. 3D Plotted PCL scaffolds for stem cell based bone tissue engineering. Macromol Symp 2008; 269:92–99.

    Article  CAS  Google Scholar 

  5. Liu TL, Miao JC, Sheng WH, Xie YF, Huang Q, Shan YB, et al. Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing. J Zhejiang Univ Sci B 2010;11:10–16.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Choi YH, Kim MG, Ahn DH, Hong SH, Lee JY, Kim HS, et al. The wound healing effect of a silk fibroin film on cutaneous burn of hairless mice. J Korean Surg Soc 2010;79:421–427.

    Article  Google Scholar 

  7. Barnett SE, Varley SJ. The effects of calcium alginate on wound healing. Ann R Coll Surg Engl 1987;69:153–155.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Wang L, Wang Y, Qu J, Hu Y, You R, Li M. The cytocompatibility of genipin-crosslinked silk fibroin films. J Biomat Nanobiotech 2013;4:213–221.

    Article  CAS  Google Scholar 

  9. Schneider A, Wang XY, Kaplan DL, Garlick JA, Egles C. Biofunctionalized electrospun silk mats as a topical bioactive dressing for accelerated wound healing. Acta Biomat 2009;5:2570–2578.

    Article  CAS  Google Scholar 

  10. Min BM, Lee G, Kim SH, Nam YS, Lee TS, Park WH. Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro. Biomaterials 2004; 25:1289–1297.

    Article  CAS  PubMed  Google Scholar 

  11. Abramo F, Argiolas S, Pisani G, Vannozzi I, Miragliotta V. Effect of a hydrocolloid dressing on first intention healing surgical wounds in the dog: a pilot study. Aust Vet J 2008;86:95–99.

    Article  CAS  PubMed  Google Scholar 

  12. Lim HJ, Kim HT, Oh EJ, Choi JH, Ghim HD, Pyun DG, et al. Effect of newly developed pectin/CMC dressing materials on three different types of wound model. Polymer Korea 2010;34:363–368.

    CAS  Google Scholar 

  13. Chakravarthy D, Rodway N, Schmidt S, Smith D, Evancho M, Sims R. Evaluation of three new hydrocolloid dressings: retention of dressing integrity and biodegradability of absorbent components attenuate inflammation. J Biomed Mater Res 1994;28:1165–1173.

    Article  CAS  PubMed  Google Scholar 

  14. Brown-Etris M, Milne C, Orsted H, Gates JL, Netsch D, Punchello M, et al. A prospective, randomized, multisite clinical evaluation of a transparent absorbent acrylic dressing and a hydrocolloid dressing in the management of Stage II and shallow Stage III pressure ulcers. Adv Skin Wound Care 2008;21:169–174.

    Article  PubMed  Google Scholar 

  15. Chen YH, Dong WR, Chen QY, Zhao BL, Zou ZZ, Xiao YQ, et al. [Biological dressing with human hair keratin-collagen sponge-poly 2-hydroxyethyl methacrylate composite promotes burn wound healing in SD rats]. Nan Fang Yi Ke Da Xue Xue Bao 2007;27:1621–1626.

    CAS  PubMed  Google Scholar 

  16. Silver GM, Robertson SW, Halerz MM, Conrad P, Supple KG, Gamelli RL. A silver-coated antimicrobial barrier dressing used postoperatively on meshed autografts: a dressing comparison study. J Burn Care Res 2007;28:715–719.

    Article  PubMed  Google Scholar 

  17. Holland AJ, Ward D, Farrell B. The influence of burn wound dressings on laser Doppler imaging assessment of a standardized cutaneous injury model. J Burn Care Res 2007;28:871–878.

    Article  PubMed  Google Scholar 

  18. Singh R, Kumar D, Kumar P, Chacharkar MP. Development and evaluation of silver-impregnated amniotic membrane as an antimicrobial burn dressing. J Burn Care Res 2008;29:64–72.

    PubMed  Google Scholar 

  19. Sezer AD, Hatipoglu F, Cevher E, Ogurtan Z, Bas AL, Akbuga J. Chitosan film containing fucoidan as a wound dressing for dermal burn healing: preparation and in vitro/in vivo evaluation. AAPS PharmSciTech 2007; 8:Article 39.

    Article  Google Scholar 

  20. Dell H. Biomaterials: silk spin-off. Nature 2006;441:821.

    Article  CAS  PubMed  Google Scholar 

  21. Minoura N, Aiba S, Higuchi M, Gotoh Y, Tsukada M, Imai Y. Attachment and growth of fibroblast cells on silk fibroin. Biochem Biophys Res Commun 1995;208:511–516.

    Article  CAS  PubMed  Google Scholar 

  22. Santin M, Motta A, Freddi G, Cannas M. In vitro evaluation of the inflammatory potential of the silk fibroin. J Biomed Mater Res 1999;46:382–389.

    Article  CAS  PubMed  Google Scholar 

  23. Sugihara A, Sugiura K, Morita H, Ninagawa T, Tubouchi K, Tobe R, et al. Promotive effects of a silk film on epidermal recovery from full-thickness skin wounds. Proc Soc Exp Biol Med 2000;225:58–64.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Chan Hum Park.

Additional information

These authors contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lee, O.J., Kim, JH., Moon, B.M. et al. Fabrication and characterization of hydrocolloid dressing with silk fibroin nanoparticles for wound healing. Tissue Eng Regen Med 13, 218–226 (2016).

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: