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Local injection of granulocyte-colony stimulating factor accelerates wound healing in a rat excisional wound model

Abstract

A systemic treatment of granulocyte-colony stimulating factor (G-CSF) is known to improve healings of damaged tissues. However, recent studies suggested local actions of G-CSF on the healing processes of damaged tissues. We investigated the treatment effect of locally injected G-CSF and compared to that of systemically injected G-CSF in a rat model. A wound was created on the rat dorsum and treated either by local injection or by systemic injection of G-CSF. Wound healing rate, deposition of collagen, and gene expression were evaluated. G-CSF receptor (G-CSFR) protein was detected by Western blotting. The wound healing rate in the local injection group was significantly higher than that in the systemic injection group at days 9 and 15; it was also significantly higher than that in the control group at days 3, 9, and 15. The expression of G-CSFR protein in wound tissues was higher than in normal skin tissues. The local injection of G-CSF is more effective than systemic injection of G-CSF in promoting wound healing, which may implicate the local action of G-CSF treatment in wound healing processes.

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Correspondence to Hyuck Kim.

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These authors contributed equally to this work.

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Shen, GY., Park, IH., Song, YS. et al. Local injection of granulocyte-colony stimulating factor accelerates wound healing in a rat excisional wound model. Tissue Eng Regen Med 13, 297–303 (2016). https://doi.org/10.1007/s13770-016-9054-9

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  • DOI: https://doi.org/10.1007/s13770-016-9054-9

Keywords

  • Granulocyte-colony stimulating factor
  • Wound healing
  • Local injection