Skip to main content
Log in

Effect of type I collagen derived from tilapia scale on odontoblast-like cells

  • Original Article
  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope


Type I collagen is the most abundant extracellular matrix in bone and dentin. It was reported to be effective in inducing the osteoblast differentiation. Majority of the type I collagen used in scientific research are from bovine skin, porcine skin and rat tail. Recently, a novel type I collagen possessing sound thermal stability, was isolated from tilapia fish scale. It was reported to be able to induce the differentiation of human mesenchymal stem cells into osteoblasts. However, no information is available regarding its effect on odontoblast-like cells. The purpose of this study was to investigate the effect of type I collagen derived from tilapia fish scale on odontoblast-like cells. Type I collagen was immobilized to tissue culture polystyrene dish. Biocompatibility of type I collagen was evaluated in terms of initial cell number, alkaline phosphatase activity (ALP activity), odontogenic gene expression and calcific deposition. The results showed that type I collagen purified from tilapia fish scale significantly enhanced the cell attachment to nearly twofold as compared to control; moreover, cells cultured in type I collagen-modified substrate was induced to differentiate toward odontoblast lineage as demonstrated by upregulation of ALP activity on day 7, enhancement of ALP, BSP mRNA expression on day 7 and 10, and accelerated mineralization on day 9. It is therefore suggested that type I collagen derived from tilapia scale, an underutilized resource, holds promise as scaffolding material in the application of tissue engineering in dental field.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others


  1. Kadler KE, Baldock C, Bella J, Boot-Handford RP. Collagens at a glance. J Cell Sci 2007;120(Pt 12):1955–1958.

    Article  CAS  PubMed  Google Scholar 

  2. Mizuno M, Miyamoto T, Wada K, Watatani S, Zhang GX. Type I collagen regulated dentin matrix protein-1 (Dmp-1) and osteocalcin (OCN) gene expression of rat dental pulp cells. J Cell Biochem 2003;88:1112–1119.

    Article  CAS  PubMed  Google Scholar 

  3. Saito T, Yamauchi M, Abiko Y, Matsuda K, Crenshaw MA. In vitro apatite induction by phosphophoryn immobilized on modified collagen fibrils. J Bone Miner Res 2000;15:1615–1619.

    Article  CAS  PubMed  Google Scholar 

  4. Primorac D, Anticevic D, Barisic I, Hudetz D, Ivkovic A. Osteogenesis imperfecta—multi-systemic and life-long disease that affects whole family. Coll Antropol 2014;38:767–772.

    PubMed  Google Scholar 

  5. Cabral WA, Perdivara I, Weis M, Terajima M, Blissett AR, Chang W, et al. Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta. PLoS Genet 2014;10:e1004465.

    Article  Google Scholar 

  6. Silva TH, Moreira-Silva J, Marques AL, Domingues A, Bayon Y, Reis RL. Marine origin collagens and its potential applications. Mar Drugs 2014;12:5881–5901.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Yamada S, Yamamoto K, Ikeda T, Yanagiguchi K, Hayashi Y. Potency of fish collagen as a scaffold for regenerative medicine. Biomed Res Int 2014;2014:302932.

    Article  PubMed Central  PubMed  Google Scholar 

  8. Matsumoto R, Uemura T, Xu Z, Yamaguchi I, Ikoma T, Tanaka J. Rapid oriented fibril formation of fish scale collagen facilitates early osteoblastic differentiation of human mesenchymal stem cells. J Biomed Mater Res A 2014 Dec 25 [Epub].

    Google Scholar 

  9. Ikoma T, Kobayashi H, Tanaka J, Walsh D, Mann S. Physical properties of type I collagen extracted from fish scales of Pagrus major and Oreochromis niloticas. Int J Biol Macromol 2003;32:199–204.

    Article  CAS  PubMed  Google Scholar 

  10. Ikoma T, Kobayashi H, Tanaka J, Walsh D, Mann S. Microstructure, mechanical, and biomimetic properties of fish scales from Pagrus major. J Struct Biol 2003;142:327–333.

    Article  PubMed  Google Scholar 

  11. Khayer Dastjerdi A, Barthelat F. Teleost fish scales amongst the toughest collagenous materials. J Mech Behav Biomed Mater 2014 Oct 6 [Epub].

    Google Scholar 

  12. Hos D, van Essen TH, Bock F, Chou CH, Pan HA, Lin CC, et al. [Decellularized collagen matrix from tilapia fish scales for corneal reconstruction (BioCornea)]. Ophthalmologe 2014;111:1027–1032.

    Article  CAS  PubMed  Google Scholar 

  13. Chou CH, Chen YG, Lin CC, Lin SM, Yang KC, Chang SH. Bioabsorbable fish scale for the internal fixation of fracture: a preliminary study. Tissue Eng Part A 2014;20:2493–2502.

    Article  CAS  PubMed  Google Scholar 

  14. Choi da J, Choi SM, Kang HY, Min HJ, Lee R, Ikram M, et al. Bioactive fish collagen/polycaprolactone composite nanofibrous scaffolds fabricated by electrospinning for 3D cell culture. J Biotechnol 2015;205:47–58.

    Article  CAS  PubMed  Google Scholar 

  15. Terada M, Izumi K, Ohnuki H, Saito T, Kato H, Yamamoto M, et al. Construction and characterization of a tissue-engineered oral mucosa equivalent based on a chitosan-fish scale collagen composite. J Biomed Mater Res B Appl Biomater 2012;100:1792–1802.

    Article  PubMed  Google Scholar 

  16. Sasai Y, Kondo S, Yamauchi Y, Kuzuya M. Immobilization of bioactive molecule onto polymer surface functionalized by plasma techniques and its application to cell culture. J Photopolym Sci Technol 2009;22:503–506.

    Article  CAS  Google Scholar 

  17. Hanks CT, Fang D, Sun Z, Edwards CA, Butler WT. Dentin-specific proteins in MDPC-23 cell line. Eur J Oral Sci 1998;106 Suppl 1:260–266.

    Article  CAS  PubMed  Google Scholar 

  18. Botero T, Otero-Corchon V, Nor J. MDPC-23: a rat odontoblast-like cell line. IADR General Session, June 20–23, IguaÇu Falls, Brazil, Abstract 328, 2012.

    Google Scholar 

  19. Cheng Y, Ramos D, Lee P, Liang D, Yu X, Kumbar SG. Collagen functionalized bioactive nanofiber matrices for osteogenic differentiation of mesenchymal stem cells: bone tissue engineering. J Biomed Nanotechnol 2014;10:287–298.

    Article  CAS  PubMed  Google Scholar 

  20. De Franceschi N, Hamidi H, Alanko J, Sahgal P, Ivaska J. Integrin traffic — the update. J Cell Sci 2015;128:839–852.

    Article  PubMed Central  PubMed  Google Scholar 

  21. Gandavarapu NR, Alge DL, Anseth KS. Osteogenic differentiation of human mesenchymal stem cells on a5 integrin binding peptide hydrogels is dependent on substrate elasticity. Biomater Sci 2014;2:352–361.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  22. Staatz WD, Fok KF, Zutter MM, Adams SP, Rodriguez BA, Santoro SA. Identification of a tetrapeptide recognition sequence for the alpha 2 beta 1 integrin in collagen. J Biol Chem 1991;266:7363–7367.

    CAS  PubMed  Google Scholar 

  23. Ozeki N, Mogi M, Kawai R, Yamaguchi H, Hiyama T, Nakata K, et al. Mouse-induced pluripotent stem cells differentiate into odontoblast-like cells with induction of altered adhesive and migratory phenotype of integrin. PLoS One 2013;8:e80026.

    Article  Google Scholar 

  24. Ozeki N, Mogi M, Yamaguchi H, Hiyama T, Kawai R, Hase N, et al. Differentiation of human skeletal muscle stem cells into odontoblasts is dependent on induction of a1 integrin expression. J Biol Chem 2014;289:14380–14391.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  25. Bassagañas S, Carvalho S, Dias AM, Pérez-Garay M, Ortiz MR, Figueras J, et al. Pancreatic cancer cell glycosylation regulates cell adhesion and invasion through the modulation of a2ß1 integrin and E-cadherin function. PLoS One 2014;9:e98595.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Jia Tang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tang, J., Saito, T. Effect of type I collagen derived from tilapia scale on odontoblast-like cells. Tissue Eng Regen Med 12, 231–238 (2015).

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:

Key Words