Tissue Engineering and Regenerative Medicine

, Volume 11, Issue 2, pp 113–120 | Cite as

Comparative Study of hydroxyapatite prepared from seashells and eggshells as a bone graft material

  • Sang-Woon Lee
  • Csaba Balázsi
  • Katalin Balázsi
  • Dong-hyun Seo
  • Han Sung Kim
  • Chang-Hyen Kim
  • Seong-Gon KimEmail author
Original Article Biomaterials


The aims of this study were to determine the physical properties of hydroxyapatite from seashells (sHA) and from eggshells (eHA), to analyze elements within sHA and eHA, and to compare the bone regeneration ability between sHA and eHA in a rat parietal bone defect model. The sHA and eHA particles had a similar morphology in scanning electron microscope images. From the Fourier-transform infrared absorbance spectra and X-ray diffraction results, both types of hydroxyapatite (HA) had the characteristics of pure HA. Inductively coupled plasma atomic emission spectroscopy results suggested that the sHA had higher levels of sodium and strontium than the eHA, whereas the eHA had higher levels of magnesium than the sHA. In μ-CT results, the mean bone mineral density of the sHA was significantly higher than the control at 4 weeks after the operation (p = 0.012). The mean bone volume of the eHA was significantly higher than the control at 8 weeks after the operation (p = 0.012). In the histological images at 4 weeks after the operation, foreign body multinucleated giant cells were observed around the agglomerated sHA particles, while there were fewer inflammatory reactions around the agglomerated eHA particles. The eHA group showed better results in bone formation than did the sHA group in this study.

Key words

seashell eggshell hydroxyapatite bone graft bone regeneration 


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Copyright information

© The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Sang-Woon Lee
    • 1
  • Csaba Balázsi
    • 2
    • 3
  • Katalin Balázsi
    • 3
  • Dong-hyun Seo
    • 4
  • Han Sung Kim
    • 4
  • Chang-Hyen Kim
    • 5
  • Seong-Gon Kim
    • 1
    Email author
  1. 1.Department of Oral and Maxillofacial Surgery, College of DentistryGangneung-Wonju National UniversityGangneungRepublic of Korea
  2. 2.Institute for Materials Science and TechnologyBay Zoltán Nonprofit Ltd. for Applied ResearchBudapestHungary
  3. 3.Institute for Technical Physics and Materials Science, Research Centre for Natural SciencesHungarian Academy of SciencesBudapestHungary
  4. 4.Department of Biomedical Engineering, Institute of Medical Engineering and Yonsei-Fraunhofer Medical Device LaboratoryYonsei UniversityWonjuRepublic of Korea
  5. 5.Department of Oral and Maxillofacial Surgery, Seoul St. Mary’s Hospital, College of MedicineThe Catholic University of KoreaSeoulRepublic of Korea

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