We previously reported that wound fluid generated from injured cornea at 5 day after injury had ability to induce mesenchymal to epithelial transition (MET) of circulating mesenchymal stem cells (MSCs). In the present study, we further analyzed wound fluid by cytokine array. Comparing with normal cornea, increased expression of chemokines CXCL2, CXCL3, CXCL5, CCL2, anti-inflammatory cytokine IL-10, growth factors VEGF and β-NGF, protease inhibitor TIMP-1 were observed in the wound fluid of injured cornea. Interestingly, the inductions of inflammatory cytokines, IL-1α, IL-1β, IL-4, IL-6, and TNFα were not detected. It suggests wound fluid at 5 day after injury consisted of beneficial mediators for wound healing. In particular, chemokine CXCL5 increased over 6 fold. We further analyzed the roles of CXCL5 using MSCs, isolated from bone marrow and human umbilical vein endothelial cells (HUVECs). Administration of CXCL5 significantly enhanced motility of MSCs as well as showed chemotactic effects on MSCs. Moreover, CXCL5 increased the endothelial proliferation and vascular density and spouting determined by vessel formation assay. Taken together, wound fluids at day 5 after injury are the pool of beneficial mediators for wound healing process.
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Chung, E., Ahn, W. & Son, Y. CXCL5 abundant in the wound fluid at the late phase of wound healing, possibly promoting migration of mesenchymal stem cells and vascular tube formation. Tissue Eng Regen Med 11, 317–322 (2014). https://doi.org/10.1007/s13770-014-0004-0
- wound fluid
- MSCs migration
- endothelial proliferation
- vessel density