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Tissue Engineering and Regenerative Medicine

, Volume 9, Issue 4, pp 185–193 | Cite as

Human umbilical cord-derived mesenchymal stem cells differentiate into ligament-like cells with mechanical stimulation in various media

  • Mi-Na Kang
  • Hee-Hoon Yoon
  • Young-Kwon Seo
  • Jung-Keug ParkEmail author
Original Article

Abstract

Applying umbilical cord mesenchymal stem cells (UC-MSCs) is extremely promising in regenerative medicine. In this study, we determined the effect of mechanical stimulation at 10% strain (5 sec of stretch and 15 sec of relaxation) and various media on differentiation of UC-MSCs into ligament-like cells over 10 days. The effects of various media, such as Dulbecco’s modified Eagle’s medium (DMEM), Advanced D-MEM (A-DMEM), and MegaCell™ DMEM (M-DMEM), were investigated. The control group was cultured in DMEM without cyclic strain. These experiments showed that the M-DMEM and A-DMEM groups were better recognized by specific interactions between extracellular matrix (ECM) molecules and membrane proteins compared to that in the DMEM and control groups. ECM production in the M-DMEM group was significantly higher than that in the other groups. Additionally, the reverse transcription polymerase chain reaction revealed that mechanical stimulation led to increased collagen-III, α-smooth muscle actin, and tenascin-C expression. Furthermore, mechanical stimulation promoted ligament-like cell differentiation of UC-MSCs, thereby inducing matrix protein expression and synthesis.

Key words

umbilical cord mesenchymal stem cell mechanical stimulation ligament-like cells differentiation 

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Copyright information

© The Korean Tissue Engineering and Regenerative Medicine Society and Springer Netherlands 2012

Authors and Affiliations

  • Mi-Na Kang
    • 1
  • Hee-Hoon Yoon
    • 1
  • Young-Kwon Seo
    • 2
  • Jung-Keug Park
    • 1
    • 2
    Email author
  1. 1.Research Institute of BiotechnologyDongguk UniversitySeoulKorea
  2. 2.Department of Medical BiotechnologyDongguk UniversitySeoulKorea

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