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MiR-124 regulates apoptosis in hypoxia-induced human brain microvessel endothelial cells through targeting Bim

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A Correction to this article was published on 08 May 2019

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Abstract

Human brain microvessel endothelial cells (HBMECs) are crucial for brain vascular repair and maintenance. The high-expressed expressions of microRNA-124 (miR-124) in brain have been investigated and revealed in many researches. In this work, we aimed to investigate the role of miR-124 in apoptosis of HBMECs and the underlying mechanism. Here, we found the low-expressed miR-124 in hypoxia-induced HBMECs using qRT-PCR analysis. MiR-124 targeting 3′-untranslated region (3′-UTR) of Bim mRNA was predicted by Targetscan database. Importantly, the decreased miR-124 expression and increased Bim expression, an opposite trend, were obtained in hypoxia-induced HBMECs. The further confirmation of the correlation between miR-124 and Bim was conducted by miR-124 overexpression and dual luciferase reporter assays. The inhibitory role of miR-124 in Bim expression was evidenced by results obtained from miR-124 overexpression analysis. Luciferase reporter assay further proved that miR-124 directly targeted the two conserved seed sites in the Bim 3′-UTR. The inhibited apoptosis of HBMECs was observed under both miR-124 overexpression and Bim knockdown condition in flow cytometry analysis. Collectively, these findings outline that miR-124 regulates apoptosis in hypoxia-induced HBMECs through targeting Bim, providing a better understanding of the role of miR-124 in apoptosis of HBMECs.

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Change history

  • 08 May 2019

    Following publication of the original article [1], the authors reported an updated affiliation for the first author, Jie Pu. The updated affiliation is shown below and reflected in the affiliation list of this Correction.

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Acknowledgments

This work was supported by General Program of Natural Science Foundation of the Inner Mongolia Autonomous region, China (No. 2015MS08151, No. 2015MS08152).

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Author notes

  1. Jie Pu, Yuan Long and Jian Zhou have contributed equally to this work.

Authors and Affiliations

  1. Department of Neurosurgery, Renmin Hospital of Wuhan University & Hubei General Hospital, Wuhan, 430060, Hubei, People’s Republic of China

    Jie Pu & Yanqiang Zhan

  2. Department of Cardiovascular Medicine, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430015, Hubei, People’s Republic of China

    Yuan Long

  3. Department of Stomatology, the 253rd Hospital of Chinese People’s Liberation Army, Hohhot, Inner Mongolia, 010051, People’s Republic of China

    Jian Zhou

  4. Department of Neurosurgery, the 253rd Hospital of Chinese People’s Liberation Army, Hohhot, Inner Mongolia, 010051, People’s Republic of China

    Xiaoyong Qin

Authors
  1. Jie Pu
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  2. Yuan Long
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  3. Jian Zhou
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  4. Yanqiang Zhan
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Correspondence to Xiaoyong Qin.

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Pu, J., Long, Y., Zhou, J. et al. MiR-124 regulates apoptosis in hypoxia-induced human brain microvessel endothelial cells through targeting Bim. Appl Biol Chem 61, 689–696 (2018). https://doi.org/10.1007/s13765-018-0407-z

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  • DOI: https://doi.org/10.1007/s13765-018-0407-z

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