Improvement in β-glucan extraction from Ganoderma lucidum with high-pressure steaming and enzymatic pre-treatment
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In this study, the high-pressure steaming and enzymatic pre-treatment (SET) was used to improve β-glucan extraction from Ganoderma lucidum (G. lucidum), an oriental medicinal mushroom. Response surface methodology and central composite design were used to determine the optimum pre-treatment conditions: high-pressure steaming, enzymatic hydrolysis, and Viscozyme L concentrations. The optimal conditions were 15.51 min for high-pressure steaming, 0.84 g/100 mL of Viscozyme L, and 4.16 h for hydrolysis. The predicted β-glucan content in G. lucidum extract at optimal conditions, approximately twofold (8.05 g/100 g) of the control treatment value, was consistent with the empirical value. The total sugar and protein contents through SET were higher than those values of the control treatment. The cell migration assay showed that SET-processed G. lucidum extracts significantly suppressed B16F10 murine melanoma cell growth. SET process using Viscozyme L could be utilized for β-glucan extraction from G. lucidum to develop the functional food.
KeywordsAnti-metastatic effect Ganoderma lucidum β-Glucan Steaming Viscozyme L
This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET) through the High Value-added Food Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (314070-3), the Government of the Republic of Korea.
- 2.Chihara G, Hamuro J, Maeda YY, Arai Y, Fukuoka F (1970) Fractionation and purification of the polysaccharides with marked antitumor activity, especially Lentinan, from Lentinus edodes (Berk.) Sing, (an Edible Mushroom)1. Cancer Res 30:2776–2781Google Scholar
- 3.Bae WC, Kim YS, Lee JW (2005) Bioactive substances from Ganoderma lucidum. Korean J Microbiol Biotechnol 33:75–83Google Scholar
- 24.Box GEP, Draper NR (1987) Empirical model-building and response surfaces. Wiley, New YorkGoogle Scholar
- 26.Motilva MJ, Serra A, Borrás X, Romero MP, Domínguez A, Labrador A, Peiró L (2014) Adaptation of the standard enzymatic protocol (Megazyme method) to microplaque format for β-(1,3)(1,4)-d-glucan determination in cereal based samples with a wide range of β-glucan content. J Cereal Sci 59:224–227CrossRefGoogle Scholar
- 28.Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Biochem 28:350–356Google Scholar
- 39.Chung H, Youn K (2005) Comparison of pretreatment methods for extraction of selected components from Ganoderma lucidum. Korean J Food Preserv 12:130–134Google Scholar
- 42.Barbieri A, Quagliariello V, Del Vecchio V, Falco M, Luciano A, Amruthraj NJ, Nasti G, Ottaiano A, Berretta M, Iaffaioli RV, Arra C (2017) Anticancer and anti-inflammatory properties of Ganoderma lucidum extract effects on melanoma and triple-negative breast cancer treatment. Nutrients 9:210CrossRefGoogle Scholar