Applied Biological Chemistry

, Volume 61, Issue 2, pp 235–242 | Cite as

Improvement in β-glucan extraction from Ganoderma lucidum with high-pressure steaming and enzymatic pre-treatment

  • In-Wook Hwang
  • Bo-Min Kim
  • Young-Chan Kim
  • Sang-Han Lee
  • Shin-Kyo Chung
Article
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Abstract

In this study, the high-pressure steaming and enzymatic pre-treatment (SET) was used to improve β-glucan extraction from Ganoderma lucidum (G. lucidum), an oriental medicinal mushroom. Response surface methodology and central composite design were used to determine the optimum pre-treatment conditions: high-pressure steaming, enzymatic hydrolysis, and Viscozyme L concentrations. The optimal conditions were 15.51 min for high-pressure steaming, 0.84 g/100 mL of Viscozyme L, and 4.16 h for hydrolysis. The predicted β-glucan content in G. lucidum extract at optimal conditions, approximately twofold (8.05 g/100 g) of the control treatment value, was consistent with the empirical value. The total sugar and protein contents through SET were higher than those values of the control treatment. The cell migration assay showed that SET-processed G. lucidum extracts significantly suppressed B16F10 murine melanoma cell growth. SET process using Viscozyme L could be utilized for β-glucan extraction from G. lucidum to develop the functional food.

Keywords

Anti-metastatic effect Ganoderma lucidum β-Glucan Steaming Viscozyme L 

Notes

Acknowledgments

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET) through the High Value-added Food Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (314070-3), the Government of the Republic of Korea.

References

  1. 1.
    Choi SJ, Lee YS, Kim JK, Kim JK, Lim SS (2010) Physiological activities of extract from edible mushrooms. J Korean Soc Food Sci Nutr 39:1087–1096CrossRefGoogle Scholar
  2. 2.
    Chihara G, Hamuro J, Maeda YY, Arai Y, Fukuoka F (1970) Fractionation and purification of the polysaccharides with marked antitumor activity, especially Lentinan, from Lentinus edodes (Berk.) Sing, (an Edible Mushroom)1. Cancer Res 30:2776–2781Google Scholar
  3. 3.
    Bae WC, Kim YS, Lee JW (2005) Bioactive substances from Ganoderma lucidum. Korean J Microbiol Biotechnol 33:75–83Google Scholar
  4. 4.
    Yang Q, Wang S, Xie Y, Sun J, Wang J (2010) HPLC analysis of Ganoderma lucidum polysaccharides and its effect on antioxidant enzymes activity and Bax, Bcl-2 expression. Int J Biol Macromol 46:167–172CrossRefGoogle Scholar
  5. 5.
    Zhong W, Liu N, Xie Y, Zhao Y, Song X, Zhong W (2013) Antioxidant and anti-aging activities of mycelial polysaccharides from Lepista sordida. Int J Biol Macromol 60:355–359CrossRefGoogle Scholar
  6. 6.
    Pang X, Chen Z, Gao X, Liu W, Slavin M, Yao W, Yu LL (2007) Potential of a novel polysaccharide preparation (GLPP) from Anhui-grown Ganoderma lucidum in tumor treatment and immunostimulation. J Food Sci 72:S435–S442CrossRefGoogle Scholar
  7. 7.
    Xu Z, Chen X, Zhong Z, Chen L, Wang Y (2011) Ganoderma lucidum polysaccharides: immunomodulation and potential anti-tumor activities. Am J Chin Med 39:15–27CrossRefGoogle Scholar
  8. 8.
    Zhu XL, Chen AF, Lin ZB (2007) Ganoderma lucidum polysaccharides enhance the function of immunological effector cells in immunosuppressed mice. J Ethnopharmacol 111:219–226CrossRefGoogle Scholar
  9. 9.
    Xiao C, Wu QP, Cai W, Tan JB, Yang XB, Zhang JM (2012) Hypoglycemic effects of Ganoderma lucidum polysaccharides in type 2 diabetic mice. Arch Pharm Res 35:1793–1801CrossRefGoogle Scholar
  10. 10.
    Zhu K, Nie S, Li C, Lin S, Xing M, Li W, Gong D, Xie M (2013) A newly identified polysaccharide from Ganoderma atrum attenuates hyperglycemia and hyperlipidemia. Int J Biol Macromol 57:142–150CrossRefGoogle Scholar
  11. 11.
    Cho JH, Lee JY, Lee MJ, Oh HN, Kang DH, Jhune CS (2013) Comparative analysis of useful β-glucan and polyphenol in the fruiting bodies of Ganoderma spp. J Mushroom 11:164–170CrossRefGoogle Scholar
  12. 12.
    Kohguchi M, Kunikata T, Watanabe H, Kudo N, Shibuya T, Ishihara T, Iwaki K, Ikeda M, Fukuda S, Kurimoto M (2004) Immuno-potentiating effects of the antler-shaped fruiting body of Ganoderma lucidum (Rokkaku-Reishi). Biosci Biotechnol Biochem 68:881–887CrossRefGoogle Scholar
  13. 13.
    Park YJ, Nam JY, Yoon DE, Kwon OC, Kim HI, Yoo YB, Kong WS, Lee CS (2013) Comparison of anti-inflammatory, antioxidant and anti-allergic effects of Ganoderma species mycelial extracts. J Mushroom 11:111–115CrossRefGoogle Scholar
  14. 14.
    Du B, Bian Z, Xu B (2014) Skin health promotion effects of natural beta-glucan derived from cereals and microorganisms: a review. Phytother Res 28:159–166CrossRefGoogle Scholar
  15. 15.
    Zhu F, Du B, Bian Z, Xu B (2015) Beta-glucans from edible and medicinal mushrooms: characteristics, physicochemical and biological activities. J Food Compos Anal 41:165–173CrossRefGoogle Scholar
  16. 16.
    Zhang M, Cui SW, Cheung PCK, Wang Q (2007) Antitumor polysaccharides from mushrooms: a review on their isolation process, structural characteristics and antitumor activity. Trends Food Sci Technol 18:4–19CrossRefGoogle Scholar
  17. 17.
    Noh JE, Yoon SR, Lim AK, Kim HJ, Huh D, Kim DI (2012) A study on the yield of functional components of citrus peel extracts using optimized hot water extraction and enzymatic hydrolysis. Korean J Food Cook Sci 28:51–55CrossRefGoogle Scholar
  18. 18.
    Lee SH, Jang GY, Kim KJ, Lee MJ, Kim TJ, Lee J, Jeong HS (2012) Effect of temperature, solvent concentration, and pH on the β-glucan extraction. Korean J Food Nutr 25:871–877CrossRefGoogle Scholar
  19. 19.
    Benito-Román Ó, Alonso E, Cocero MJ, Goto M (2016) β-Glucan recovery from Ganoderma lucidum by means of pressurized hot water and supercritical CO2. Food Bioprod Process 98:21–28CrossRefGoogle Scholar
  20. 20.
    Gil-Ramírez A, Clavijo C, Palanisamy M, Ruiz-Rodríguez A, Navarro-Rubio M, Marin FR, Reglero G, Soler-Rivas C (2013) Screening of edible mushrooms and extraction by pressurized water (PWE) of 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitors. J Funct Foods 5:244–250CrossRefGoogle Scholar
  21. 21.
    Smiderle FR, Morales D, Gil-Ramírez A, de Jesus LI, Gilbert-López B, Iacomini M, Soler-Rivas C (2017) Evaluation of microwave-assisted and pressurized liquid extractions to obtain β-d-glucans from mushrooms. Carbohydr Polym 156:165–174CrossRefGoogle Scholar
  22. 22.
    Benito-Román Ó, Alvarez VH, Alonso E, Cocero MJ, Saldaña MDA (2015) Pressurized aqueous ethanol extraction of β-glucans and phenolic compounds from waxy barley. Food Res Int 75:252–259CrossRefGoogle Scholar
  23. 23.
    Zheng HZ, Hwang IW, Chung SK (2009) Enhancing polyphenol extraction from unripe apples by carbohydrate-hydrolyzing enzymes. J Zhejiang Univ Sci B 10:912–919CrossRefGoogle Scholar
  24. 24.
    Box GEP, Draper NR (1987) Empirical model-building and response surfaces. Wiley, New YorkGoogle Scholar
  25. 25.
    Lee HJ, Jung SK, Do JR, Kim HK (2015) Optimization of extraction conditions of sarcodon aspratus by response surface methodology. J Korean Soc Food Sci Nutr 44:464–469CrossRefGoogle Scholar
  26. 26.
    Motilva MJ, Serra A, Borrás X, Romero MP, Domínguez A, Labrador A, Peiró L (2014) Adaptation of the standard enzymatic protocol (Megazyme method) to microplaque format for β-(1,3)(1,4)-d-glucan determination in cereal based samples with a wide range of β-glucan content. J Cereal Sci 59:224–227CrossRefGoogle Scholar
  27. 27.
    Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson DC, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150:76–85CrossRefGoogle Scholar
  28. 28.
    Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Biochem 28:350–356Google Scholar
  29. 29.
    Lee OH, Lee HB, Lee J, Son JY, Rhee SK, Kim HD, Kim YC, Lee BY (2005) Chemical properties of olive and bay leaves. J Korean Soc Food Sci Nutr 34:503–508CrossRefGoogle Scholar
  30. 30.
    Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63CrossRefGoogle Scholar
  31. 31.
    Heo JC, Park JY, Lee JM, Kwon TK, Kim SU, Chung SK, Lee SH (2005) Wisteria floribunda gall extract inhibits cell migration in mouse B16F1 melanoma cells by regulating CD44 expression and GTP-RhoA activity. J Ethnopharmacol 102:10–14CrossRefGoogle Scholar
  32. 32.
    Xu Y, Cai F, Yu Z, Zhang L, Li X, Yang Y, Liu G (2016) Optimisation of pressurised water extraction of polysaccharides from blackcurrant and its antioxidant activity. Food Chem 194:650–658CrossRefGoogle Scholar
  33. 33.
    Ma C, Feng M, Zhai X, Hu M, You L, Luo W, Zhao M (2013) Optimization for the extraction of polysaccharides from Ganoderma lucidum and their antioxidant and antiproliferative activities. J Taiwan Inst Chem Eng 44:886–894CrossRefGoogle Scholar
  34. 34.
    Mendes LC, de Menezes HC, Aparecida M, da Silva A (2001) Optimization of the roasting of robusta coffee (C. canephora conillon) using acceptability tests and RSM. Food Qual Prefer 12:153–162CrossRefGoogle Scholar
  35. 35.
    Zheng HZ, Hwang IW, Kim SK, Lee SH, Chung SK (2010) Optimization of carbohydrate-hydrolyzing enzyme aided polyphenol extraction from unripe apples. J Korean Soc Appl Biol Chem 53:342–350CrossRefGoogle Scholar
  36. 36.
    Guan X, Yao HY (2008) Optimization of Viscozyme L assisted extraction of oat bran protein using response surface methodology. Food Chem 106:345–351CrossRefGoogle Scholar
  37. 37.
    Hwang IW, Chung SK, Jeong MC, Chung HS, Zheng HZ (2013) Optimization of enzymatic hydrolysis of persimmon peels for vinegar fermentation. J Korean Soc Appl Biol Chem 56:435–440CrossRefGoogle Scholar
  38. 38.
    Matsunaga Y, Wahyudiono S, Machmudah S, Sasaki M, Goto M (2014) Hot compressed water extraction of polysaccharides from Ganoderma lucidum using a semibatch reactor. Asia Pac J Chem Eng 9:125–133CrossRefGoogle Scholar
  39. 39.
    Chung H, Youn K (2005) Comparison of pretreatment methods for extraction of selected components from Ganoderma lucidum. Korean J Food Preserv 12:130–134Google Scholar
  40. 40.
    Lai L, Yang D (2007) Rheological properties of the hot-water extracted polysaccharides in Ling-Zhi (Ganoderma lucidum). Food Hydrocoll 21:739–746CrossRefGoogle Scholar
  41. 41.
    Wan-Mohtar WAAQ, Young L, Abbott GM, Clements C, Harvey LM, McNeil B (2016) Antimicrobial properties and cytotoxicity of sulfated (1, 3)-β-d-glucan from the mycelium of the mushroom Ganoderma lucidum. J Microbiol Biotechnol 26:999–1010CrossRefGoogle Scholar
  42. 42.
    Barbieri A, Quagliariello V, Del Vecchio V, Falco M, Luciano A, Amruthraj NJ, Nasti G, Ottaiano A, Berretta M, Iaffaioli RV, Arra C (2017) Anticancer and anti-inflammatory properties of Ganoderma lucidum extract effects on melanoma and triple-negative breast cancer treatment. Nutrients 9:210CrossRefGoogle Scholar
  43. 43.
    Loganathan J, Jiang J, Smith A, Jedinak A, Thyagarajan-Sahu A, Sandusky GE, Nakshatri H, Sliva D (2014) The mushroom Ganoderma lucidum suppresses breast-to-lung cancer metastasis through the inhibition of pro-invasive genes. Int J Oncol 44:2009–2015CrossRefGoogle Scholar

Copyright information

© The Korean Society for Applied Biological Chemistry 2018

Authors and Affiliations

  • In-Wook Hwang
    • 1
  • Bo-Min Kim
    • 2
  • Young-Chan Kim
    • 3
  • Sang-Han Lee
    • 2
  • Shin-Kyo Chung
    • 2
    • 4
  1. 1.Department of Food Science and NutritionDong-A UniversityBusanRepublic of Korea
  2. 2.School of Food Science and BiotechnologyKyungpook National UniversityDaeguRepublic of Korea
  3. 3.Korea Food Research InstituteWanju-gunRepublic of Korea
  4. 4.Food and Bio-Industry Research InstituteKyungpook National UniversityDaeguRepublic of Korea

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