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Applied Biological Chemistry

, Volume 61, Issue 2, pp 173–180 | Cite as

Isolation of Burkholderia cepacia JBK9 with plant growth-promoting activity while producing pyrrolnitrin antagonistic to plant fungal diseases

  • Byung Kwon Jung
  • Sung-Jun Hong
  • Gun-Seok Park
  • Min-Chul Kim
  • Jae-Ho Shin
Article
  • 206 Downloads

Abstract

Burkholderia species are widely distributed across wide ecological niches. Many genera of Burkholderia are known to be associated with plants and are involved in processes such as suppression of soil-borne pathogens, acceleration of plant growth and endophytic colonization. In the present study, a strain belonging to the Burkholderia cepacia complex, which was termed JBK9, was isolated. The strain JBK9 showed broad-spectrum antifungal activities against Phytophthora capsici, Fusarium oxysporum, and Rhizoctonia solani, which are representative phytopathogenic fungi, inhibiting their growth by 59.56, 51.92, and 34.22%, respectively. The strain produced an antifungal compound that was confirmed to be pyrrolnitrin by TLC, HPLC, and NMR analyses. Using an in vitro assay for plant root colonization, we observed that the population densities of B. cepacia JBK9 on the upper 1 cm of host plant roots were significantly different between Burkholderia species. The high motility of these strains is likely to have contributed to their efficient root colonization. The isolated strain was evaluated in vivo for its ability to control Phytophthora blight via a pot test. Compared with Burkholderia strains KCTC 2973 and ATCC 25416, B. cepacia JBK9 demonstrated a stronger antifungal activity against P. capsici. The strain B. cepacia JBK9 could be further developed as a biological control agent for pepper plants.

Keywords

Antifungal activity Biological control Burkholderia cepacia Pyrrolnitrin 

Notes

Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), Funded by the Ministry of Education (NRF-2015R1D1A1A01057187).

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Copyright information

© The Korean Society for Applied Biological Chemistry 2018

Authors and Affiliations

  1. 1.School of Applied Biosciences, College of Agriculture and Life SciencesKyungpook National UniversityDaeguRepublic of Korea
  2. 2.Department of Biomedical EngineeringThe University of Texas at AustinAustinUSA
  3. 3.Institute of Agricultural Science and TechnologyKyungpook National UniversityDaeguRepublic of Korea

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