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Applied Biological Chemistry

, Volume 61, Issue 1, pp 125–130 | Cite as

Validation protocol for whole-body dosimetry in an agricultural exposure study

  • Jiho Lee
  • Eunhye Kim
  • Yongho Shin
  • Jonghwa Lee
  • Junghak Lee
  • Wolfgang Maasfeld
  • Jeong-Han Kim
Note
  • 126 Downloads

Abstract

Agricultural workers exposed to pesticides can experience adverse health impacts depending on toxicity and exposure amount. Whole-body dosimetry (WBD) is the most reliable, practical, and realistic method for measuring exposure. Since validation of analytical and experimental methodologies is critical for quantitative determination of exposure, we conducted a validation procedure to design an essential protocol for WBD exposure studies. Using the fungicide kresoxim-methyl, matrix-matched standards were prepared with various matrices including outer cloth, inner cloth, washing solution for gloves and hands, gauze, and glass fiber filter (IOM sampler) to determine the instrumental limit of quantitation for high-performance liquid chromatography (HPLC) (2 ng) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) (10 pg). Method limits of quantitation (MLOQ) were also set for HPLC (0.1 mg/L) and LC–MS/MS (0.005 mg/L). We observed good analysis repeatability (coefficient of variation < 6%), and the linearity of the calibration curves was reasonable (r 2 > 0.998) in the range of 0.001–10 mg/L in various matrices. Recovery tests were carried out at three levels of concentration (MLOQ, 10 MLOQ, and 100 MLOQ) and resulted in good recoveries (72.7–105.6%). We did not observe breakthrough of the compound in tests of holding capacity for glass fiber pesticide filters. The procedures established in the present study are applicable as an essential, comprehensive protocol for exposure assessment studies using WBD.

Keywords

Exposure IOM sampler Kresoxim-methyl Method validation Pesticide Whole-body dosimetry 

Notes

Acknowledgments

This study was financially supported by the Rural Development Administration (PJ009948).

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Copyright information

© The Korean Society for Applied Biological Chemistry 2017

Authors and Affiliations

  1. 1.Department of Agricultural Biotechnology, Research Institute of Agriculture and Life SciencesSeoul National UniversitySeoulRepublic of Korea
  2. 2.Bayer CropScienceMonheimGermany

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