Determination of flavonoids from Perilla frutescens var. japonica seeds and their inhibitory effect on aldose reductase
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Perilla frutescens var. japonica (PF) is an annual aromatic herb has been consumed as a food ingredient and medicinal crop in Asian countries. To evaluate the therapeutic efficacy of aldose reductase (AR) inhibition, we tested the PF seeds. The stepwise polarities of PF were tested for AR inhibition, and we determined the CH2Cl2 and EtOAc fractions to be good inhibitors (5.81 and 3.99 μg/mL, respectively). Compounds 1–3 were isolated from the CH2Cl2 and EtOAc fractions and identified as luteolin (1), apigenin (2), and diosmetin (3) by physicochemical and spectroscopic data. Among them, luteolin (1) and apigenin (2) had high AR inhibitory activity (1.89 and 4.18 μM). Deulsaem, a variety of PF, was determined to have the highest flavonoid content among ten PF seeds tested (2.10 mg/g). This study suggests that PF could be utilized as a natural source to treat diabetic complications.
KeywordsAldose reductase Diabetic complications Perilla frutescens var. japonica
This work was supported by the Chung-Ang University Research Grants in 2017 and grants from Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ010156022016), Rural Development Administration, Republic of Korea.
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