Applied Biological Chemistry

, Volume 60, Issue 2, pp 155–162 | Cite as

Determination of flavonoids from Perilla frutescens var. japonica seeds and their inhibitory effect on aldose reductase

  • Jaemin Lee
  • Joyce P. Rodriguez
  • Norman G. Quilantang
  • Myoung-Hee Lee
  • Eun Ju Cho
  • Sonia D. Jacinto
  • Sanghyun Lee


Perilla frutescens var. japonica (PF) is an annual aromatic herb has been consumed as a food ingredient and medicinal crop in Asian countries. To evaluate the therapeutic efficacy of aldose reductase (AR) inhibition, we tested the PF seeds. The stepwise polarities of PF were tested for AR inhibition, and we determined the CH2Cl2 and EtOAc fractions to be good inhibitors (5.81 and 3.99 μg/mL, respectively). Compounds 13 were isolated from the CH2Cl2 and EtOAc fractions and identified as luteolin (1), apigenin (2), and diosmetin (3) by physicochemical and spectroscopic data. Among them, luteolin (1) and apigenin (2) had high AR inhibitory activity (1.89 and 4.18 μM). Deulsaem, a variety of PF, was determined to have the highest flavonoid content among ten PF seeds tested (2.10 mg/g). This study suggests that PF could be utilized as a natural source to treat diabetic complications.


Aldose reductase Diabetic complications Perilla frutescens var. japonica 



This work was supported by the Chung-Ang University Research Grants in 2017 and grants from Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ010156022016), Rural Development Administration, Republic of Korea.


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Copyright information

© The Korean Society for Applied Biological Chemistry 2017

Authors and Affiliations

  • Jaemin Lee
    • 1
  • Joyce P. Rodriguez
    • 1
  • Norman G. Quilantang
    • 1
  • Myoung-Hee Lee
    • 2
  • Eun Ju Cho
    • 3
  • Sonia D. Jacinto
    • 4
  • Sanghyun Lee
    • 1
  1. 1.Department of Integrative Plant ScienceChung-Ang UniversityAnseongRepublic of Korea
  2. 2.Department of Southern Area Crop Science, National Institute of Crop ScienceRural Development AdministrationMiryangRepublic of Korea
  3. 3.Department of Food Science and NutritionPusan National UniversityBusanRepublic of Korea
  4. 4.Institute of BiologyUniversity of the Philippines, DilimanQuezon CityPhilippines

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