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Antioxidant and cognitive-enhancing activities of Arctium lappa L. roots in Aβ1-42-induced mouse model

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Abstract

Many studies have shown that oxidative stress induced by hydrogen peroxide can lead to neuronal cell death. In this study, protective effects of the ethanol extract of A. lappa L. roots against oxidative stress in PC12 cells were measured by 2′, 7′-dichlorofluorescein diacetate and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Oxidative stress contributes to the memory deficits induced by Beta-amyloid1-42 in mice. Treatment with the ethanol extract of A. lappa L. roots improved working and reference memory in mice in the Y-maze and passive avoidance tests. To detect the lipid peroxidation levels, malondialdehyde was measured. To purify the bioactive compound, active fractions and components of A. lappa L. roots were isolated by partitioning, open column chromatography, TLC, high-performance liquid chromatography, electron ionization mass spectrometry, and 1H/13C-nuclear magnetic resonance. We identified the bioactive compound as quinic acid, which is a powerful antioxidant agent. Ultimately, the roots of A. lappa L. may become available as an antioxidant food for Alzheimer’s disease patients and those with other oxidative stress-induced disorders.

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Acknowledgments

This research was supported by a Korea University Grant.

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Authors and Affiliations

  1. Department of Food and Biotechnology, Korea University, 612 College of Life Sciences, Green Campus, 145 Anam-ro, Seongbuk-gu, Seoul, 136-701, Republic of Korea

    Yoon Kyung Kwon & Dong-Hoon Shin

  2. Functional Food Research Center, Korea University, Seoul, 130-701, Republic of Korea

    Soo Jung Choi & Cho Rong Kim

  3. Department of Food Science and Nutrition, University of Minnesota, St. Paul, Minnesota, Minneapolis, MN, 55455, USA

    Jae Kyeom Kim

  4. College of Nursing Science, Kyung Hee University, Seoul, 130-701, Republic of Korea

    Youn-Jung Kim

  5. Natural Way Co., Ltd, Gyeonggi-do, 487-030, Republic of Korea

    Jong Hun Choi

  6. Department of Family Medicine, St. Vincent’s Hospital, College of Medicine, The Catholic University of Korea, Suwon, 442-723, Republic of Korea

    Sang-Wook Song

  7. Department of Physiology, Kyung Hee University, Seoul, 130-701, Republic of Korea

    Chang-Ju Kim

  8. Department of Food Science and Biotechnology, GaChon University, Gyeonggi-do, 461-701, Republic of Korea

    Gwi Gun Park

  9. School of Medicine, Department of Microbiology, Kyung Hee University, Seoul, 130-701, Republic of Korea

    Cheung-Seog Park

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  1. Yoon Kyung Kwon
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  2. Soo Jung Choi
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Correspondence to Dong-Hoon Shin.

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Kwon, Y.K., Choi, S.J., Kim, C.R. et al. Antioxidant and cognitive-enhancing activities of Arctium lappa L. roots in Aβ1-42-induced mouse model. Appl Biol Chem 59, 553–565 (2016). https://doi.org/10.1007/s13765-016-0195-2

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