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In vitro and in vivo effects of polysaccharides isolated from Korean persimmon vinegar on intestinal immunity

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Abstract

The in vitro and in vivo effects of crude polysaccharide isolated from Korean persimmon vinegar (KPV-0) on the intestinal immunostimulation were examined. KPV-0 was not cytotoxic to intestinal epithelial Caco-2 cells and could be transported through a Caco-2 cell monolayer in an in vitro coculture system. For and in vitro experiment, KPV-0 treatment significantly increased IgA production by Peyer’s patch (PP) cells and triggered an increase in transforming growth factor (TGF)-β1 and interleukin (IL)-6 levels. To investigate the in vivo effects of KPV-0 treatment, KPV-0 was administered to mice orally at different doses for 20 days. Oral administration of KPV-0-induced IgA and cytokines (IL-6, granulocyte macrophage-colony-stimulating factor, and TGF-β) production by PP cells and significantly increased the IgA levels in intestinal fluids and feces. These results suggested that the polysaccharides isolated from persimmon vinegar appear to modulate the intestinal immune system and could be beneficial to human health.

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References

  • Brandtzaeg P (2010) Function of mucosa-associated lymphoid tissue in antibody formation. Immunol Invest 39:303–355

    Article  CAS  Google Scholar 

  • Cerutti A, Rescigno M (2008) The biology of intestinal immunoglobulin A responses. Immunity 28:740–750

    Article  CAS  Google Scholar 

  • Courts FL (2013) Profiling of modified citrus pectin oligosaccharide transport across Caco-2 cell monolayers. Pharmanutrition 1:22–31

    Article  CAS  Google Scholar 

  • Defrance T, Vanbervliet B, Briere F, Durand I, Rousset F, Banchereau J (1992) Interleukin 10 and transforming growth factor beta cooperate to induce anti-CD40-activated naive human B cells to secrete immunoglobulin A. J Exp Med 175:671–682

    Article  CAS  Google Scholar 

  • Fan L, Ding S, Ai L, Deng K (2012) Antitumor and immunomodulatory activity of water-soluble polysaccharide from Inonotus obliquus. Carbohydr Polym 90:870–874

    Article  CAS  Google Scholar 

  • Fayette J, Dubois B, Vandenabeele S, Bridon JM, Vanbervliet B, Durand I, Banchereau J, Caux C, Brière F (1997) Human dendritic cells skew isotype switching of CD40-activated naive B cells towards IgA1 and IgA2. J Exp Med 185:1909–1918

    Article  CAS  Google Scholar 

  • Giudici P, Gullo M, Solieri L (2009) Traditional balsamic vinegar. In: Solieri L, Giudici P (eds) Vinegars of the world. Springer, Milan, pp 157–177

    Chapter  Google Scholar 

  • Gonnella PA, Chen Y, Inobe J, Komagata Y, Quartulli M, Weiner HL (1998) In situ immune response in gut-associated lymphoid tissue (GALT) following oral antigen in TCR-transgenic mice. J Immunol 160:4708–4718

    CAS  Google Scholar 

  • He B, Xu W, Santini PA, Polydorides AD, Chiu A, Estrella J, Shan M, Chadburn A, Villanacci V, Plebani A, Knowles DM, Rescigno M, Cerutti A (2007) Intestinal bacteria trigger T cell-independent immunoglobulin A(2) class switching by inducing epithelial-cell secretion of the cytokine APRIL. Immunity 26:812–826

    Article  CAS  Google Scholar 

  • Hwang YC, Shin KS (2008) Characterization of immuno-stimulating polysaccharides isolated from Korean persimmon vinegar. Korean J Food Sci Technol 40:220–227

    Google Scholar 

  • Kim DS, Shin KS (2014) Chemical property and macrophage stimulating activity of polysaccharides isolated from brown rice and persimmon vinegars. Korean J Food Nutr 27:1033–1042

    Article  Google Scholar 

  • Kim DS, Hurh BS, Shin KS (2015) Chemical characteristics and immuno-stimulatory activity of polysaccharides from fermented vinegars manufactured with different raw materials. J Korean Soc Food Sci Nutr 44:191–199

    Article  CAS  Google Scholar 

  • Kiyohara H, Uchida T, Takakiwa M, Matsuzaki T, Hada N, Takeda T, Shibata T, Yamada H (2010) Different contributions of side-chains in beta-d-(1→3,6)-galactans on intestinal Peyer’s patch-immunomodulation by polysaccharides from Astragalus mongholics Bunge. Phytochemistry 71:280–293

    Article  CAS  Google Scholar 

  • Liu CW, Yang DJ, Chang YY, Hsu CL, Tseng JK, Chang MH, Wang M, Chen YC (2012) Polyphenol-rich longan (Dimocarpus longan Lour.)-flower-water-extract attenuates nonalcoholic fatty liver via decreasing lipid peroxidation and downregulating matrix metalloproteinases-2 and -9. Food Res Int 45:444–449

    Article  CAS  Google Scholar 

  • Macpherson AJ, McCoy KD, Johansen FE, Brandtzaeg P (2008) The immune geography of IgA induction and function. Mucosal Immunol 1:11–22

    Article  CAS  Google Scholar 

  • Mantis NJ, Rol N, Corthésy B (2011) Secretory IgA’s complex roles in immunity and mucosal homeostasis in the gut. Mucosal Immunol 4:603–611

    Article  CAS  Google Scholar 

  • Matsuo T, Ito S (1978) The chemical structure of kaki-tannin from immature fruit of the persimmon (Diospyros kaki L.). Agric Biol Chem 42:1637–1643

    Article  CAS  Google Scholar 

  • Murooka Y, Nanda K, Yamashita M (2009) Rice vinegars. In: Solieri L, Giudici P (eds) Vinegars of the world. Springer, Milan, pp 121–133

    Chapter  Google Scholar 

  • Newberry RD, Lorenz RG (2005) Organizing a mucosal defense. Immunol Rev 206:6–21

    Article  CAS  Google Scholar 

  • Okahashi N, Yamamoto M, Vancott JL, Chatfield SN, Roberts M, Bluethmann H, Hiroi T, Kiyono H, McGhee JR (1996) Oral immunization of interleukin-4 (IL-4) knockout mice with a recombinant Salmonella strain or cholera toxin reveals that CD4 + Th2 cells producing IL-6 and IL-10 are associated with mucosal immunoglobulin A responses. Infect Immun 64:1516–1525

    CAS  Google Scholar 

  • Osada K, Suzuki T, Kawakami Y, Senda M, Kasai A, Sami M, Ohta Y, Kanda T, Ikeda M (2006) Dose-dependent hypocholesterolemic actions of dietary apple polyphenol in rats fed cholesterol. Lipids 41:133–139

    Article  CAS  Google Scholar 

  • Paulsen BS, Barsett H (2005) Bioactive Pectic Polysaccharides. In: Heinze T (ed) Polysaccharides I, vol 186. Springer, Heidelberg, pp 69–101

    Chapter  Google Scholar 

  • Peters M, Solem F, Goldschmidt J, Schirmacher P, Rose-John S (2001) Interleukin-6 and the soluble interleukin-6 receptor induce stem cell factor and Flt-3L expression in vivo and in vitro. Exp Hematol 29:146–155

    Article  CAS  Google Scholar 

  • Rainieri S, Zambonelli C (2009) Organisms associated with acetic acid bacteria in vinegar production. In: Solieri L, Giudici P (eds) Vinegars of the world. Springer, Milan, pp 73–95

    Chapter  Google Scholar 

  • Ramberg J, Nelson E, Sinnott R (2010) Immunomodulatory dietary polysaccharides: a systematic review of the literature. Nutr J 9:54

    Article  Google Scholar 

  • Ridley BL, O’Neill ML, Mohnen D (2001) Pectins: structure, biosynthesis, and oligogalacturonide-related signaling. Phytochemistry 57:929–967

    Article  CAS  Google Scholar 

  • Sakanaka S, Tachibana Y, Okada Y (2005) Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha). Food Chem 89:569–575

    Article  CAS  Google Scholar 

  • Sato A, Hashiguchi M, Toda E, Iwasaki A, Hachimura S, Kaminogawa S (2003) CD11b+ Peyer’s patch dendritic cells secrete IL-6 and induce IgA secretion from naive B cells. J Immunol 171:3684–3690

    Article  CAS  Google Scholar 

  • Schepetkin IA, Quinn MT (2006) Botanical polysaccharides: macrophage immunomodulation and therapeutic potential. Int Immunopharmacol 6:317–333

    Article  CAS  Google Scholar 

  • Shi Y, Liu CH, Roberts AI, Das J, Xu G, Ren G, Zhang Y, Yuan ZR, Tan HS, Das G, Devadas S (2006) Granulocyte-macrophage colony-stimulating factor (GM-CSF) and T-cell responses: what we do and don’t know. Cell Res 16:126–133

    Article  CAS  Google Scholar 

  • Tanoue T, Nishitani Y, Kanazawa K, Hashimoto T, Mizuno M (2008) In vitro model to estimate gut inflammation using co-cultured Caco-2 and RAW264.7 cells. Biochem Biophys Res Commun 374:565–569

    Article  CAS  Google Scholar 

  • Vacek A, Hofer M, Hola J, Weiterová L, Štreitová D, Svoboda J (2007) The role of G-CSF and IL-6 in the granulopoiesis-stimulating activity of murine blood serum induced by perorally administered ultrafiltered pig leukocyte extract, IMUNOR. Int Immunopharmacol 7:656–661

    Article  CAS  Google Scholar 

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Acknowledgments

This study was supported by Kyonggi University’s Graduate Research Assistantship 2014. Also, a part of this study was supported by The Globalization of Korean Foods R&D program, the Ministry of Agriculture, and Food and Rural Affairs. We express our sincere gratitude for the support.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Kyonggi University, San 94-6, Ieu-dong, Youngtong-gu, Suwon, Gyeonggi-do, 443-760, Republic of Korea

    Min Young Lee & Kwang Soon Shin

  2. Department of Integrated Biomedical and Life Science, Korea University, Anam-ro 145, Seongbuk-gu, Seoul, 136-701, Republic of Korea

    Hoon Kim

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  1. Min Young Lee
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  2. Hoon Kim
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Correspondence to Kwang Soon Shin.

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Lee, M.Y., Kim, H. & Shin, K.S. In vitro and in vivo effects of polysaccharides isolated from Korean persimmon vinegar on intestinal immunity. J Korean Soc Appl Biol Chem 58, 867–876 (2015). https://doi.org/10.1007/s13765-015-0117-8

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