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Plant-derived molecules from Saussurea grandifolia as inhibitors of aldose reductase

Abstract

Saussurea grandifolia was tested for aldose reductase (AR) inhibition as part of a search for potentially therapeutic natural compounds. Stepwise polarity fractions were tested for in vitro inhibition of rat lens AR. Of these, the ethyl acetate (EtOAc) and n-butanol (n-BuOH) fractions exhibited AR inhibitory activities. A chromatographic separation of the active EtOAc and n-BuOH fractions led to the isolation of nine compounds, which were known and identified as vitexin (1), genkwanin-5-O-glucoside (2), vitexin 2″-p-hydroxybenzoate (3), kaempferol-3-O-rutinoside (4), isovitexin (5), quercitrin (6), isoquercitrin (7), 1,5-di-O-caffeoylquinic acid (8), and vicenin 1 (9). Among these compounds, compounds 6, 7, and 8 showed very strong inhibitory activities against AR (IC50’s = 0.34, 0.32, and 0.08 μM, respectively). The results suggest that S. grandifolia is clearly a potential source for therapeutic agent that might be useful for the treatment of diabetic complications.

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Acknowledgments

This work was supported by grants from Scientific Research of Korea National Arboretum (KNA1-2-13, 11-5), Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ010156022014), Rural Development Administration, and iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries), Ministry of Agriculture, Food, and Rural Affairs, Republic of Korea. We thank the National Center for Inter-University Research Facilities (Seoul National University, Republic of Korea) for the NMR and MS measurements.

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Correspondence to Sanghyun Lee.

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Kim, H.M., Lee, D.G. & Lee, S. Plant-derived molecules from Saussurea grandifolia as inhibitors of aldose reductase. J Korean Soc Appl Biol Chem 58, 365–371 (2015). https://doi.org/10.1007/s13765-015-0051-9

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Keywords

  • Saussurea grandifolia
  • Aldose reductase
  • Flavonoid
  • Phenylpropanoid