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Identification of flavonoids from Eriodictyon californicum and their cytotoxicity against HCT116 colon cancer cells


The extract of Eriodictyon californicum has been widely used as an essential emollient, and as an anti-inflammatory, antibacterial, and antifungal agent. In this study, antitumor effects of E. californicum were evaluated against HCT116 human colon cancer cells. Treatment with the ethyl acetate extract of E. californicum (EEC) inhibited the short-term viability and long-term clonogenicity of HCT116 cells. We identified several flavonoids, including rosmarinic acid and luteolin, from the EEC by using high-performance liquid chromatography and nuclear magnetic resonance. Luteolin inhibited clonogenicity, activated caspase-7, and induced apoptosis of HCT116 cells. These findings suggest that EEC contains bioactive flavonoids that exhibit antitumor activities.

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  • Danial NN, Korsmeyer SJ (2004) Cell death: critical control points. Cell 116:205–219

    Article  CAS  Google Scholar 

  • Fletcher JN, Kinghorn AD, Slack JP, McCluskey TS, Odley A, Jia Z (2011) In vitro evaluation of flavonoids from Eriodictyon californicum for antagonist activity against the bitterness receptor hTAS2R31. J Agric Food Chem 59:13117–13121

    Article  CAS  Google Scholar 

  • Franken NA, Rodermond HM, Stap J, Haveman J, van Bree C (2006) Clonogenic assay of cells in vitro. Nat Protoc 1:2315–2319

    Article  CAS  Google Scholar 

  • Hwang D, Jo G, Hyun J, Lee SD, Koh D, Lim Y (2012) Synthesis of methoxybenzoflavones and assignments of their NMR data. Magn Reson Chem 50:62–67

    Article  CAS  Google Scholar 

  • Ley JP, Krammer G, Reinders G, Gatfield IL, Bertram HJ (2005) Evaluation of bitter masking flavanones from Herba Santa (Eriodictyon californicum (H. and A.) Torr., Hydrophyllaceae). J Agric Food Chem 53:6061–6066

    Article  CAS  Google Scholar 

  • Liu YL, Ho DK, Cassady JM, Cook VM, Baird WM (1992) Isolation of potential cancer chemopreventive agents from Eriodictyon californicum. J Nat Prod 55:357–363

    Article  CAS  Google Scholar 

  • Othman NH (2012) Honey and cancer: sustainable inverse relationship particularly for developing nations-a review. Evid Based Complement Alternat Med 2012:410406. doi:10.1155/2012/410406

  • Shin SY, Kim CG, Kim SH, Kim YS, Lim Y, Lee YH (2010) Chlorpromazine activates p21Waf1/Cip1 gene transcription via early growth response-1 (Egr-1) in C6 glioma cells. Exp Mol Med 42:395–405

    Article  CAS  Google Scholar 

  • Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C (1995) A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods 184:39–51

    Article  CAS  Google Scholar 

  • Wenzel U, Kuntz S, Brendel MD, Daniel H (2000) Dietary flavone is a potent apoptosis inducer in human colon carcinoma cells. Cancer Res 60:3823–3831

    CAS  Google Scholar 

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This paper resulted from the Konkuk University Research Support Program.

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Correspondence to Young Han Lee.

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Y. Yong and S.Y. Shin have contributed equally to this work.

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Shin, S.Y., Yong, Y., Hong, D.S. et al. Identification of flavonoids from Eriodictyon californicum and their cytotoxicity against HCT116 colon cancer cells. J Korean Soc Appl Biol Chem 58, 77–81 (2015).

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  • Eriodictyon californicum
  • Antitumor activity
  • Flavonoid
  • Caspase
  • Apoptosis
  • Colon cancer