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Fructooligosaccharides decreased the ability of probiotic Escherichia coli Nissle 1917 to adhere to co-cultures of human intestinal cell lines

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Abstract

In the present study, we investigated effects of prebiotic fructooligosaccharides (FOS) on the ability of Escherichia coli Nissle 1917 (EcN), a probiotic bacterial strain, to adhere to co-cultures of human intestinal cells (Caco-2 and HT29-MTX). For the bacterial adhesion assay, the co-cultures were seeded together at a ratio of 3:1 and then incubated EcN in the presence of various prebiotics including FOS. To eliminate possible bias, both manual and automated cell counting methods were utilized. In results, FOS significantly suppressed the adhesion abilities of probiotic EcN to the co-cultures. Taken altogether, we carefully suggest that an unbalanced supplementation of prebiotics may adversely impact on gut microflora; hence, further investigations are warranted in regards to such unexpected adverse effects and responsible mechanisms on colonization of probiotics in the gastrointestinal tract utilizing in vivo models.

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Abbreviations

ATCC:

American Type Culture Collection

ANOVA:

Analysis of variance

DMSO:

Dimethyl sulfoxide

DMEM:

Dulbecco’s Modified Eagle Media

EcN:

Escherichia coli Nissle 1917

FOS:

Fructooligosaccharide

FRU:

Fructose

GI:

Gastrointestinal

GFP:

Green fluorescent protein

INU:

Inulin

LB:

Luria–Bertani

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PBS:

Phosphate-buffered saline

SUC:

Sucrose

pGFPuv:

UV-variant green fluorescent protein

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Acknowledgments

This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (Ministry of Education, Science, and Technology (357-2011-1-F00040).

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Authors and Affiliations

  1. Department of Food Science and Nutrition, University of Minnesota, Saint Paul, MN, 55108, USA

    J. K. Kim

  2. Department of Food Science, Gyeongnam National University of Science and Technology, Jinju, 660-758, Republic of Korea

    E. C. Shin

  3. Division of Nutritional Sciences, Cornell University, Ithaca, NY, 14853, USA

    H. G. Park

Authors
  1. J. K. Kim
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  2. E. C. Shin
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  3. H. G. Park
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Correspondence to H. G. Park.

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Kim, J.K., Shin, E.C. & Park, H.G. Fructooligosaccharides decreased the ability of probiotic Escherichia coli Nissle 1917 to adhere to co-cultures of human intestinal cell lines. J Korean Soc Appl Biol Chem 58, 45–52 (2015). https://doi.org/10.1007/s13765-015-0002-5

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