The selected isolate, Bacillus sp. SW1-1 showed antibacterial activity against both Gram-positive and Gram-negative bacteria involved in fish diseases, including Edwardsiella tarda, Streptococcus iniae, S. parauberis, Vibrio anguillarum, and V. harveyi. The Maximum bacteriocin production was observed at 30°C after 24 h with brain heart infusion medium (pH 7.0). The bacteriocin SW1-1 was purified by 50% ammonium sulfate precipitation, followed by HiPrep diethylaminoethyl 16/10 FF and Sephacryl S-100 High resolution column chromatography. The substance was characterized as a bacteriocin-like inhibitory substance with a molecular mass of 38 kDa. Bacteriocin SW1-1 was sensitive to the proteolytic action of pepsin, trypsin, chymotrypsin, and protease types I and XIV, and relatively heat labile, despite the fact that bacteriocin activity was still detected after heating at 100°C for 30 min. The activity of bacteriocin SW1-1 was stable in the pH range of 2.0–11.0, and relatively unaffected by organic chemicals. The bacteriocin SW1-1 had a bacteriolytic mechanism, resulting in cell wall degradation of E. tarda. These characteristics indicate that this bacteriocin may be a potential candidate for alternative agent to control important pathogens of fish diseases in aquaculture.
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Abriouel H, Franz CMAP, Omar NB, and Gálvez A. (2011) Diversity and applications of Bacillus bacteriocins. FEMS Microbiol Rev 35, 201–32.
Ansari A, Aman A, Siddiqui NN, Iqbal S, and Aliul Qader S. (2012) Bacteriocin (BAC-IB17): screening. Isolation and production from Bacillus subtilis KIBGE IB-17. Pak J Pham Sci 25, 195–201.
Bansemir A, Maja B, Susanne S, and Ulrike L. (2006) Screening of cultivated seaweeds for antibacterial activity against fish pathogenic bacteria. Aquarculture 252, 79–84.
Bastos MCF, Coutinho BG, and Coelho MLV. (2010) Lysostaphin: A Staphylococcal bacteriolysin with potential clinical applications. Pharmaceuticals 3, 1139–61.
Bizani D and Brandelli A. (2002) Characterization of a bacteriocin produced by a newly isolated Bacillus sp. 8A. J Appl Microbiol 93, 512–9.
Bronneke V and Fiedler F. (1994) Production of bacteriolytic enzymes by Streptomyces globisporus regulated by exogenous bacterial cell walls. Appl Env Microbiol 60, 785–91.
Cladera-Olivera F, Caron GR, and Brandelli A. (2004) Bacteriocin-like substance production by Bacillus licheniformis strain P40. Lett Appl Microbiol 38, 251–6.
Cotter PD, Hill C, and Ross RP. (2006) “What’s in a name ? Class distinction for bacteriocins”. Nat Rev Microbiol 4. DOI:10.1038/nrmicro1273-c1.
Diep DB and Nes IF. (2002) Ribosomally synthesized antibacterial peptides in gram positive bacteria. Curr Drug T 3, 107–22.
Favret ME and Youston AA. (1989) Thuricin: The bacteriocin produced by Bacillus thuringiensis. J Invertebr Pathol 53, 206–16.
Halami PM, Chandrashekar A, and Joseph R. (1999) Characterization of bacteriocinogenic strains of lactic acid bacteria in fowl and fish intestines and mushroom. Food Biotechnol 13, 121–36.
Hancock REW. (1997) Peptides antibiotics. Lancet 349, 418–22.
Holo H, Nilssen Ø, and Nes IF. (1991) Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene. J Bacteriol 173, 3879–87.
Hyronimus B, Le Marrec C, and Urdacim MC. (1998) Coagulin, a bacteriocin-like inhibitory substance produced by Bacillus coagulans I4. J Appl Microbiol 85, 42–50.
Jansen EF and Hirschmann DJ. (1944) Subtilin, an antibacterial substance of Bacillus subtilis: Culturing condition and properties. Arch Biochem 4, 297–309.
Kim SO, Ohk SH, Bai DH, and Yu JH. (1999) Purification and properties of bacteriolytic enzymes from Bacillus licheniformis YS-1005 against Streptococcus mutans. Biosci Biotechnol Biochem 63, 73–7.
Kiss A, Balikó G, Csorba A, Chuluunbaatar T, Medzihradszky KF, and Alföldi L. (2008) Cloning and characterization of the DNA region responsible for megacin A-216 production in Bacillus megaterium 216. J Bacteriol 190, 6448–57.
Klein C, Kaletta C, and Entian KD. (1993) Biosynthesis of the lantibiotic subthlin is regulated by a histidine kinase/response regulator system. Appl Environ Micorbiol 59, 296–303.
Klein C, Kaletta C, Schnell N, and Entian KD. (1992) Analysis of genes involved in biosynthesis of the lantibiotic subtilin. Appl Environ Microbiol 58, 132–42.
Krieg A. (1970) Thuricin, a bacteriocin produced by Bacillus thuriniensis. J Invertebr Pathol 15, 291.
Kumar S, Tamura K, Jakobsen IB, and Nei M. (2001) MEGA2: Molecular evolutionary genetics analysis software. Bioinformatics 12, 1244–5.
Laemmli UK. (1970) Cleavage of structural protein during the assembly of the lead of bacteriophage T4. Nature 227, 680–5.
Lee HS and Lee HJ. (2011) Purification and Biochemical Characterization of Bacteriolytic Enzyme from Bacillus subtilis YU-1432 Active against Porphyromonas gingivalis. J Korean Soc Appl Biol Chem 54, 600–5.
Lim JH, Jeong HY, and Kim SD. (2011) Characterization of the bacteriocin J4 produced by Bacillus amyloliquefaciens J4 isolated from Korean traditional fermented soybean paste. J Korean Soc Appl Biol Chem 54, 468–74.
Lim SM, Park MY, and Chang DS. (2005) Characterization of bacteriocin produced by Enterococcus faecium MJ-14 isolated from Meju. Food Sci Biotechnol 14, 49–57.
Moroni O, Kheadr E, Boutin Y, Lacroix C, and Fliss I. (2006) Inactivation of adhesion and invasion of food-borne Listeria monocytogenes by Bacteriocin-producing Bifidobacterium strains of human origin. Appl Environ Microbiol 72, 6894–901.
Motta AS and Brandelli A. (2002) Characterization of an antibacterial peptide produced by Brevibacterium linens. J Appl Micribiol 92, 63–70.
Naclerio G, Ricca E, Sacco M, and Felice D. (1993) Antimicrobial activity of a newly identified bacteriocin of Bacillus cereus. Appl Environ Microbiol 59, 4313–16.
Nilsen T, Nes IF, and Holo H. (2003) Enterolysin A, a cell wall degrading bacteriocin from Enterococcus faecalis LMG 2333. Appl Environ Microbiol 69, 2975–84.
Nishio C, Komura S, and Kurahashi K. (1983) Peptide antibiotic subtilin is synthesized via precursor proteins. Biochem Biophys Res Commun 16, 751–8.
O’Sullivan L, Ross RP, and Hill C. (2002) Potential of bacteriocin-producing lactic acid bacteria for improvement in food safety and quality. Biochimie 84, 593–604.
Paik HD and Glatz BA. (1997) Enhanced bacteriocin production by Propionibacterium thoenii in fed-batch fermentation. J Food Prot 60, 1529–33.
Paik HD, Bae SS, Park SH, and Pan JG. (1997) Identification and partial characterization of tochicin, a bacteriocin produced by Bacillus thuringiensis subsp. tochigiensis. J Ind Microbiol Biotechnol 19, 294–8.
Parente E and Ricciardi A. (1994) Influence of pH on the production of enterocin 1146 during batch fermentation. Lett Appl Microbiol 19, 12–5.
Rice KC and Bayles KW. (2008) Molecular control of bacterial death and lysis. Microbiol. Mol Rev 72, 85–109.
Saito H and Miura KI. (1963) Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–29.
Shevchenko A, Jensenm O, Podtelejnikov AV, Sagliocco F, Willm M, Vorm O, Mortensen P, Shevchenko A, Boucherie H, and Mann M. (1996) Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels. Pro Natl Acad Sci USA 93, 14440–5.
Sugita S, Hirose Y, Matsuo N, and Deguchi Y. (1998) Production of the antibacterial substance by Bacillus sp. Strain NM 12, an intestinal bacterium of Japanese coastal fish. Aquaculture 165, 269–80.
Tagg JR and McGiven AR. (1971) Assay system for bacteriocins. Appl Microbiol 21, 943.
Thompson JD, Higgins DG, and Gibson TJ. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acid Res 22, 4673–80.
Von Döhren H. (1995) Peptides. In Genetics and Biochemistry of Antibiotic Production, Vining LC and Stuttard C (eds.), pp. 129–71. Newton, MA: Butterworth-Heinemann, USA.
Von Tersch MA and Carlton BC. (1983) Bacteriocin from Bacillus megaterium ATCC 19213: Comparative studies with megacin A-216. J Bacteriol 155, 866–71.
Xie J, Zhang R, Shang C, and Guo Y. (2009) Isolation and characterization of a bacteriocin produced by an isolated Bacillus subtilis LFB112 that exhibits antimicrobial activity against domestic animal pathogens. Afr J ai]Biotechnol 8, 5611–19.
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Kim, YO., Park, IS., Kim, DJ. et al. Identification and characterization of a bacteriocin produced by an isolated Bacillus sp. SW1-1 that exhibits antibacterial activity against fish pathogens. J Korean Soc Appl Biol Chem 57, 605–612 (2014). https://doi.org/10.1007/s13765-014-4174-1
- antibacterial activity
- fish pathogenic bacteria