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Expression of IFN-γ induced by CpG-DNA stimulation in a human myeloid leukemia cell line KG-1

  • Dongbum Kim
  • Younghee Lee
  • Hyung-Joo KwonEmail author
Original Article Biochemistry/Molecular Biology

Abstract

CpG-DNA has potent immunostimulatory effects to induce expression of cytokines and chemokines in macrophages, dendritic cells, and B cells. KG-1 is a human myeloid leukemia cell line used as an in vitro model system for dendritic cell differentiation. Here, we investigated effect of CpG-DNA on IFN-γ expression in KG-1 cells. Based on the mRNA and protein expression, stimulation of KG-1 cells with the phosphorothioated form of CpG-DNA induced expression of IFN-γ. The IFN-γ promoter includes one NF-κB binding site and two AP-1 binding sites. Induction of IFN-γ was markedly reduced in the presence of NF-κB, p38, and MEK1 inhibitors. Furthermore, AP-1 was found to interact with both of the two binding sites in the IFN-γ promoter. Therefore, we conclude that CpG-DNA induced activation of NF-κB and AP-1 leads to induction of IFN-γ, which may further contribute to immunostimulatory activity of CpG-DNA.

Keywords

AP-1 CpG-DNA IFN-γ NF-κB promoter 

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Copyright information

© The Korean Society for Applied Biological Chemistry 2013

Authors and Affiliations

  1. 1.Center for Medical Science Research, College of MedicineHallym UniversityChuncheonRepublic of Korea
  2. 2.Department of Biochemistry, College of Natural SciencesChungbuk National UniversityCheongjuRepublic of Korea
  3. 3.Center for Medical Science Research, Department of Microbiology, College of MedicineHallym UniversityChuncheonRepublic of Korea

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