Skip to main content
SpringerLink
Log in

Antinociceptive effect of glyasperin F isolated from Glycyrrhiza inflata in mice

Journal of the Korean Society for Applied Biological Chemistry volume 56, pages 541–545 (2013)Cite this article

Abstract

Antinociceptive effect of glyasperin F isolated from Glycyrrhiza inflata extract (GIE) in ICR mice was studied. Oral administration of GIE (1–100 mg/kg) caused a dose-dependent reduction in acetic acid-induced writhing responses. To identify the active antinociceptive compound from the GIE, sub-fractions were obtained from the EtOAc layer of GIE by using a medium pressure liquid chromatography. From the sub-fractions obtained, the sub-fraction, which, when administered orally (10 mg/kg) showed an antinociceptive effect in both the writhing test and second phase of the formalin test was identified as glyasperin F using NMR and MS analyses. Finally, the antinociceptive effect of glyasperin F in mouse models of pain was confirmed. Orally administered glyasperin F (0.1–10mg/kg) showed a dose-dependent antinociceptive effect in both the writhing test and second phase of the formalin test. Taken together, glyasperin F isolated from the GIE may be used as a leading compound for further studies on pain and as a new drug derived from natural products for pain therapy.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

References

  • Aly AM, Al-Alousi L, and Salem HA (2005) Licorice: a possible antiinflammatory and anti-ulcer drug. AAPS PharmSciTech 6, E74–E82.

    Article  Google Scholar 

  • Asl MN and Hosseinzadeh H (2008) Review of pharmacological effects of Glycyrrhiza sp. and its bioactive compounds. Phytother Res 22, 709–724.

    Article  CAS  Google Scholar 

  • Cho YC, Lee SH, Yoon G, Kim HS, Na JY, Choi HJ et al. (2010) Licochalcone E reduces chronic allergic contact dermatitis and inhibits IL-12p40 production through down-regulation of NF-kappa B. Int Immunopharmacol 10, 1119–1126.

    Article  CAS  Google Scholar 

  • Dao TT, Nguyen PH, Lee HS, Kim E, Park J, Lim SI et al. (2011) Chalcones as novel influenza A (H1N1) neuraminidase inhibitors from Glycyrrhiza inflata. Bioorg Med Chem Lett 21, 294–298.

    Article  CAS  Google Scholar 

  • Dhingra D, Parle M, and Kulkarni SK (2004) Memory enhancing activity of Glycyrrhiza glabra in mice. J Ethnopharmacol 91, 361–365.

    Article  Google Scholar 

  • Dhingra D and Sharma A (2006) Antidepressant-like activity of Glycyrrhiza glabra L. in mouse models of immobility tests. Prog Neuropsychopharmacol Biol Psychiatry 30, 449–454.

    Article  Google Scholar 

  • Di Mambro VM and Fonseca MJ (2005) Assays of physical stability and antioxidant activity of a topical formulation added with different plant extracts. J Pharm Biomed Anal 37, 287–295.

    Article  Google Scholar 

  • Gareri P, Condorelli D, Belluardo N, Russo E, Loiacono A, Barresi V et al. (2004) Anticonvulsant effects of carbenoxolone in genetically epilepsy prone rats (GEPRs). Neuropharmacology 47, 1205–1216.

    Article  CAS  Google Scholar 

  • Haraguchi H, Ishikawa H, Mizutani K, Tamura Y, and Kinoshita T (1998a) Antioxidative and superoxide scavenging activities of retrochalcones in Glycyrrhiza inflata. Bioorg Med Chem 6, 339–347.

    Article  CAS  Google Scholar 

  • Haraguchi H, Tanimoto K, Tamura Y, Mizutani K, and Kinoshita T (1998b) Mode of antibacterial action of retrochalcones from Glycyrrhiza inflata. Phytochemistry 48, 125–129.

    Article  CAS  Google Scholar 

  • Hatano T, Shintani Y, Aga Y, Shiota S, Tsuchiya T, and Yoshida T (2000) Phenolic constituents of licorice. VIII. Structures of glicophenone and glicoisoflavanone, and effects of licorice phenolics on methicillinresistant Staphylococcus aureus. Chem Pharm Bull (Tokyo) 48, 1286–1292.

    Article  CAS  Google Scholar 

  • Hunskaar S and Hole K (1987) The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain 30, 103–114.

    Article  CAS  Google Scholar 

  • Kim HJ, Seo SH, Lee BG, and Lee YS (2005) Identification of tyrosinase inhibitors from Glycyrrhiza uralensis. Planta Med 71, 785–787.

    Article  CAS  Google Scholar 

  • Kim YH, Shin EK, Kim DH, Lee HH, Park JH, and Kim JK (2010) Antiangiogenic effect of licochalcone A. Biochem Pharmacol 80, 1152–1159.

    Article  CAS  Google Scholar 

  • Koster R, Anderson M, and Beer EJ (1959) Acetic acid for analgesic screening. Fed Proc 18, 412.

    Google Scholar 

  • Kwon HS, Park JH, Kim DH, Kim YH, Park JH, Shin HK et al. (2008) Licochalcone A isolated from licorice suppresses lipopolysaccharide-stimulated inflammatory reactions in RAW264.7 cells and endotoxin shock in mice. J Mol Med (Berl) 86, 1287–1295.

    Article  CAS  Google Scholar 

  • Le Bars D, Gozariu M, and Cadden SW (2001) Animal models of nociception. Pharmacol Rev 53, 597–652.

    Google Scholar 

  • Lee HN, Cho HJ, Lim do Y, Kang YH, Lee KW, and Park JH (2013) Mechanisms by which licochalcone e exhibits potent anti-inflammatory properties: studies with phorbol ester-treated mouse skin and lipopolysaccharide-stimulated murine macrophages. Int J Mol Sci 14, 10926–10943.

    Article  CAS  Google Scholar 

  • Nagai H, He JX, Tani T, and Akao T (2007) Antispasmodic activity of licochalcone A, a species-specific ingredient of Glycyrrhiza inflata roots. J Pharm Pharmacol 59, 1421–1426.

    Article  CAS  Google Scholar 

  • Ofir R, Tamir S, Khatib S, and Vaya J (2003) Inhibition of serotonin re-uptake by licorice constituents. J Mol Neurosci 20, 135–140.

    Article  CAS  Google Scholar 

  • Park HG, Bak EJ, Woo GH, Kim JM, Quan Z, Kim JM et al. (2012) Licochalcone E has an antidiabetic effect. J Nutr Biochem 23, 759–767.

    Article  CAS  Google Scholar 

  • Saeedi M, Morteza-Semnani K, and Ghoreishi MR (2003) The treatment of atopic dermatitis with licorice gel. J Dermatolog Treat 14, 153–157.

    Article  CAS  Google Scholar 

  • Shi Y, Wu D, Sun Z, Yang J, Chai H, Tang L et al. (2012) Analgesic and uterine relaxant effects of isoliquiritigenin, a flavone from Glycyrrhiza glabra. Phytother Res 26, 1410–1417.

    Article  CAS  Google Scholar 

  • Shibata S, Inoue H, Iwata S, Ma RD, Yu LJ, Ueyama H et al. (1991) Inhibitory effects of licochalcone A isolated from Glycyrrhiza inflata root on inflammatory ear edema and tumour promotion in mice. Planta Med 57, 221–224.

    Article  CAS  Google Scholar 

  • Tjolsen A, Berge OG, Hunskaar S, Rosland JH, and Hole K (1992) The formalin test: an evaluation of the method. Pain 51, 5–17.

    Article  CAS  Google Scholar 

  • Tsukiyama R, Katsura H, Tokuriki N, and Kobayashi M (2002) Antibacterial activity of licochalcone A against spore-forming bacteria. Antimicrob Agents Chemother 46, 1226–1230.

    Article  CAS  Google Scholar 

  • Vyklicky L (1979) The techniques for the study of pain in animals. In In Advances In Pain Research And Theraphy, Bonica JJ, Liebeskind JC, and Albe-Fessard DG (eds.), Raven 3, 727–745, USA

    Google Scholar 

  • Wang ZY and Nixon DW (2001) Licorice and cancer. Nutr Cancer 39, 1–11.

    Article  CAS  Google Scholar 

  • Yoon G, Kang BY, and Cheon SH (2007) Topoisomerase I inhibition and cytotoxicity of licochalcones A and E from Glycyrrhiza inflata. Arch Pharm Res 30, 313–316.

    Article  CAS  Google Scholar 

  • Zeng L, Fukai T, Nomura T, Zhang RY, and Lou ZC (1992) Five New Isoprenoid-substituted Flavonoids, Glyasperins F, G, H, I, and J from the Roots of Glycyrrhiza aspera. Heterocycles 34, 1813–1828.

    Article  CAS  Google Scholar 

  • Zhan C and Yang J (2006) Protective effects of isoliquiritigenin in transient middle cerebral artery occlusion-induced focal cerebral ischemia in rats. Pharmacol Res 53, 303–309.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion, Suwon, 443-766, Republic of Korea

    Jin Kyu Kim & Joa Sub Oh

  2. College of Pharmacy, Dankook University, Cheonan, 330-714, Republic of Korea

    Joa Sub Oh

  3. Department of Pharmacology, College of Medicine, Dankook University, Cheonan, 330-714, Republic of Korea

    Jin-Koo Lee

Authors
  1. Jin Kyu Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Joa Sub Oh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jin-Koo Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jin-Koo Lee.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kim, J.K., Oh, J.S. & Lee, JK. Antinociceptive effect of glyasperin F isolated from Glycyrrhiza inflata in mice. J Korean Soc Appl Biol Chem 56, 541–545 (2013). https://doi.org/10.1007/s13765-013-3161-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13765-013-3161-2

Keywords

search

Navigation