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Effect of soy phosphatidylserine supplemented diet on skin wrinkle and moisture in Vivo and clinical trial

Abstract

Effect of supplementation of phosphatidylserine (PS), enzymatically synthesized from soy lecithin, on skin moisture and wrinkle in animal and human was investigated. Skin moisture content of hairless mice was significantly decreased and skin thickness and wrinkle were significantly increased by UV irradiation, whereas PS-supplemented groups showed reduced wrinkle depth and much less wrinkle area unlike UV control (UV/Con) group. The collagen content in PS-supplemented groups increased compared with that in UV/Con group. A placebo-controlled double-blind intake study of soft capsules containing PS (300 mg/day) was performed on 63 subjects who had normal healthy skin for 12 weeks. Dermatologist’s visual assessment and image analysis of replicas showed that wrinkle near eye was improved by PSsupplementation. Upon measurement of moisture content in the skin, PS supplementation increased the moisture content in the skin. These findings showed that PS supplementation was effective in moisturizing and improving wrinkle of the skin in both animal and human; thus, PS could be used as an effective skin food ingredient.

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References

  • Assefa Z, Garmyn M, Bouillon R, Merlevede W, Vandenheede JR, and Agostinis P (1997) Differential stimulation of ERK and JNK activities by ultraviolet B irradiation and epidermal growth factor in human keratinocytes. J Invest Dermatol 108, 886–891.

    CAS  Article  Google Scholar 

  • Brennan M, Bhatti H, Nerusu KC, Bhagavathula N, Kang S, Fisher GJ et al. (2003) Matrix metalloproteinase-1 is the major collagenolytic enzyme responsible for collagen damage in UV-irradiated human skin. Photochem Photobiol 78, 43–48.

    CAS  Article  Google Scholar 

  • Cho S, Kim HH, Lee MJ, Lee S, Park CS, Nam SJ et al. (2008) Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo. J Lipid Res 49, 1235–1245.

    CAS  Article  Google Scholar 

  • Choi S (2002) Epidermis proliferative effect of the panax ginseng ginscnoside Rb2. Arch Pharm Res 25, 71–76.

    CAS  Article  Google Scholar 

  • Chung JH, Lee SH, Youn CS, Park BJ, Kim KH, Park KC et al. (2001) Cutaneous Photodamage in Koreans: Influence of sex, sun exposure, smoking, and skin color. Arch Dermatol 137, 1043–1051.

    CAS  Google Scholar 

  • Chung SY, Nam SJ, Choi WK, Seo MY, Kim JW, Lee SH et al. (2006) Phosphatidylserine enhances skin barrier function through keratinocyte differentiation. J Soc Cosmet Scientists Korea 32, 17–22.

    Google Scholar 

  • Davis BH, Chen A, and Beno DW (1996) Raf and mitogen-activated protein kinase regulate stellate cell collagen gene expression. J Biol Chem 271, 11039–11042.

    CAS  Article  Google Scholar 

  • De Spirt S, Sies H, Tronnier H, and Heinrich U (2012) An encapsulated fruit and vegetable juice concentrate increases skin microcirculation in healthy women. Skin Phamacol Physiol 25, 2–8.

    Article  Google Scholar 

  • Fini ME, Cook JR, Mohan R, and Brinckerhoff CE (1988) Regulation of matrix metalloproteinase gene expression. In Matrix metalloproteinases, Parks WC, and Mecham RP (ed), pp. 299–359, Academic, USA.

    Google Scholar 

  • Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, Kang S et al. (1996) Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature 379, 335–339.

    CAS  Article  Google Scholar 

  • Fisher GJ, and Voorhees JJ (1998) Molecular mechanisms of photoaging and its prevention by retinoic acid: ultraviolet irradiation induces MAP kinase signaltransduction cascades that induce Ap-1-regulated matrix metalloproteinases that degrade human skin in vivo. J Invest Dermatol Symp Proc 3, 61–68.

    CAS  Article  Google Scholar 

  • Gail J (2002) Molecular mechanism of skin ageing. Mech Ageing Dev 123, 801–810.

    Article  Google Scholar 

  • Guillou S, Ghabri S, Jannot C, Gaillard E, Lamour I, and Boisnic S (2011) The moisturizing effect of a wheat extract food supplement on women’s skin: a randomized, double-blind placebo-controlled trial. Int J Cosmet Sci 33, 138–143.

    CAS  Article  Google Scholar 

  • Haratake A, Uchida Y, and Schmuth M (1997) UVB-induced alterations in permeability barrier function: roles for epidermal hyperproliferation and the thymocyte-mediated response. J Invest Dermatol 108, 769–775.

    CAS  Article  Google Scholar 

  • Huang C, Ma WY, Dawson MI, Rincon M, Flavell RA, and Dong Z (1997) Blocking activator protein 1 activity, but not activation retinoic response effect of retinoic acid. Proc Acad Sci 94, 5826–5832.

    CAS  Article  Google Scholar 

  • Im SJ, Kim KN, You YG, Lee JC, Mun YJ, Kim JH et al. (2003) Effect of radix Ginseng and radix Trichosanthis on the melanogenesis. Biol Pham Bull 26, 849–853.

    CAS  Article  Google Scholar 

  • Improta-Brears T, Ghosh S, and Bell RM (1999) Mutational analysis of Raf-1 cysteine rich domain: requirement for a cluster of basic aminoacids for interaction with phosphatidylserine. Mol Cell Biochem 198, 171–178.

    CAS  Article  Google Scholar 

  • Kang JS, Kim HN, Jung DJ, Kim JE, Mun GH, Kim YS et al. (2007) Regulation of UVB-induced IL-8 and MCP-1 production in skin keratinocytes by increasing vitamin C uptake via the redistribution of SVCT-1 from the cytosol to the membrane. J Invest Dermatol 127, 698–706.

    CAS  Article  Google Scholar 

  • Kippenberger S, Loitsch SM, Grundmann-Kollmann M, Simon S, Dang TA, Hardt-Weinelt K et al. (2001) Activators of peroxisome proliferatoractivated receptors protect human skin from ultraviolet-B-light-induced inflammation. J Invest Dermatol 117, 1430–1436.

    CAS  Article  Google Scholar 

  • Kuenzli S, and Saurat JH (2003) Peroxisome proliferator activated receptors in cutaneous biology. Br J Dermatol 149, 229–236.

    CAS  Article  Google Scholar 

  • Lee EH, Cho SY, Kim SJ, Shin ES, Chang HK, Kim DH et al. (2003) Ginsenoside F1 protects human HaCaT keratinocytes from ultraviolet-Binduced apoptosis by maintaining constant levels of Bcl-2. J Invest Dermatol 121, 607–613.

    CAS  Article  Google Scholar 

  • Li JJ, Dong Z, Dawson MI, and Colburn NH (1996) Inhibition of tumor promoter-induced transformation by retinoids the transrepress AP-1 without trasnsactivation retinoic acid response element. Cancer Res 56, 483–489.

    CAS  Google Scholar 

  • Mac-Mary S, Creidi P, Marsaut D, Courderot-Masuyer C, Cochet V, Gharbi T et al. (2006) Assessment of effects of an additional dietary natural mineral water uptake on skin hydration in healthy subjects by dynamic barrier function measurements and clinic scoring. Skin Res Technol 12, 199–205.

    CAS  Article  Google Scholar 

  • Meinhard W, Iliana TP, Lale N, Wenjian M, Lars AS, Ziba RW et al. (2001) Solar UV irradiation and dermal photoaging. J Photochem Photobiol B Biol 63, 41–51.

    Article  Google Scholar 

  • Paepe KD, Lagarde JM, Gall Y, Roseeuw D, and Rogiers V (2000) Microrelief of the skin using a light transmission method. Arch Dermatol. Res 292, 500–510.

    Article  Google Scholar 

  • Park SN (2003) Antioxidative properties of baicalein, component from Scutellaria baicalensis Georgi and its application to cosmetics (I). J Korean Ind Eng Chem 14, 657–662.

    CAS  Google Scholar 

  • Seo JY, Choi HR, Rhie GE, Youn CS, Choi WW, Kim JA et al. (2001) The effect of retinoic acid and vitamin C on the expression of the procollagen a1 (I), tropoelastin, and MMP-1 in human dermal fibroblast. Korean J Invest Dermtol 8, 23–28.

    Google Scholar 

  • Tomiuk S, Zumbansen M, and Stoffel W (2000) Characterization and subcellular localization of murine and human magnesium-dependent neutral sphingomyelinase. J. Biol. Chem 275, 5710–5717.

    CAS  Article  Google Scholar 

  • Van Veldhoven PP, Mannaerts GP, Declercq P, and Baes M (2000) Do sphingoid bases interact with the peroxisome proliferator activated receptor alpha (PPAR-alpha)?. Cell Signal 12, 475–479.

    Article  Google Scholar 

  • Vance J, and Steenbergen R (2005) Metabolism and functions of phosphatidylserine. Prog Lipid Res 44, 207–234.

    CAS  Article  Google Scholar 

  • Verdaguer N, Corbalan-Garcia S, Ochoa WF, Fita I, and Gómez-Fernández JC (1999) Ca(2+) bridges the C2 membrane-binding domain of protein kinase Calpha directly to phosphatidylserine. Embo J 18, 6329–6338.

    CAS  Article  Google Scholar 

  • Yeung T, Gilbert GE, Shi J, Silvius J, Kapus A, and Grinstein S (2008) Membrane phosphatidylserine regulates surface charge and protein localization. Sci 319, 210–213.

    CAS  Article  Google Scholar 

  • Yoshinori T, Yukiko Y, and Michio K (1995) The relationship between agerelated changes in the physical properties and development of wrinkles in human facial skin. J Soc Cosmet Scientists Korea 46, 163–173.

    Google Scholar 

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Correspondence to Hee-Don Choi or Jeong-Jun Han.

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Choi, HD., Han, JJ., Yang, JH. et al. Effect of soy phosphatidylserine supplemented diet on skin wrinkle and moisture in Vivo and clinical trial. J Korean Soc Appl Biol Chem 56, 227–235 (2013). https://doi.org/10.1007/s13765-013-3012-1

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  • DOI: https://doi.org/10.1007/s13765-013-3012-1

Keywords

  • anti-wrinkle
  • moisturizing effect
  • phosphatidylserine
  • skin barrier function
  • supplementation