Optimization of process variables for the synthesis of silver nanoparticles by Pycnoporus sanguineus using statistical experimental design
- 287 Downloads
Sequential optimization strategy based on statistical experimental design and one-factor-at-a-time (OFAT) method were employed to optimize the process parameters for the enhancement of silver nanoparticles (AgNPs) production through biological synthesis using Pycnoporus sanguineus. Based on the OFAT method, three significant components influencing the size of AgNPs produced were identified as AgNO3 concentration, incubation temperature, and agitation speed. The optimum values of these process parameter for the synthesis of AgNPs were determined using response surface methodology (RSM) based on Box-Behnken design. The validity of the model developed was verified, and the statistical analysis showed that the optimum operating conditions were 0.001 M of AgNO3, 38°C, and 200 rpm with the smallest AgNPs produced at 14.86 nm. The disc diffusion method also suggested that AgNPs produced using optimum conditions have higher antimicrobial activity compared to the unoptimized AgNPs. The present study developed a robust operating condition for the production of AgNPs by P. sanguineus, which was 8.6-fold smaller than that obtained from un-optimized conditions.
KeywordsBox-Behnken optimization Pycnoporus sanguineus silver nanoparticles (AgNPs)
Unable to display preview. Download preview PDF.
- Beveridge TJ, Hughes MN, Lee H, Leung KT, Poole RK, Savvaidis I et al. (1996) Metal-Microbe Interactions: Contemporary Approaches. In Advances in Microbial Physiology. Poole RK (ed.), vol. 38, pp. 177–243. Academic Press, New York, USA.Google Scholar
- Brett DW (2006) A discussion of silver as an antimicrobial agent: alleviating the confusion. Ostomy Wound Manag 52, 34–41.Google Scholar
- Chan YS and Mashitah MD (2012) Instantaneous Biosynthesis of Silver Nanoparticles by Selected Macro Fungi. J Basic Appl Sci 6, 222–226.Google Scholar
- Kaviya S, Santhanalakshmi J, and Viswanathan B (2011) Green synthesis of silver nanoparticles using Polyalthia longifolia leaf extract along with d-sorbitol study of antibacterial activity. J Nanotechnology 2012, ID 152970.Google Scholar
- Mokhtari N, Daneshpajouh S, Seyedbagheri S, Atashdehghan R, Abdi K, Sarkar S et al. (2009) Biological synthesis of very small silver nanoparticles by culture supernatant of Klebsiella pneumonia: The effects of visible-light irradiation and the liquid mixing process. Mater Res Bull 44, 1415–1421.CrossRefGoogle Scholar
- Morozkina E, Kurakov A, Nosikov A, Sapova E, and L’vov N (2005) Properties of nitrate reductase from Fusarium oxysporum 11dn1 fungi grown under aerobic and anaerobic condition. Prikl Biokhim Mikrobiol 41, 292–297.Google Scholar
- Nabeel MA, Sastry KS, and Mohan PM (2005) Biosorption of silver ions by processed Aspergillus niger biomass. Biotechnol Lett 17, 551–556.Google Scholar
- Ottow JCG and von Klopotek A (1969) Enzymatic reduction of iron oxide by fungi. Appl Microbiol 18, 41–43.Google Scholar
- Redinbaugh MG and Campbell WH (1985) Quaternary Structure and Composition of Squash NADH: Nitrate Reductase. J Biol Chem 260, 3380–3385.Google Scholar
- Souza Anderson S, dos Santos Walter N, and Ferreira Sergio L (2005) Application of Box-Behnken design in the optimization of an on-line pre-concentration system using knotted reactor for cadmium determination by flame atomic absorption spectrometry. Spectrochim Acta B 607, 737–742.CrossRefGoogle Scholar