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Efficiency and microbial diversity of PHBV-supported solid-phase denitrification system bio-augmented with Diaphorobacter polyhydroxybutyrativorans bacteria at low temperatures

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Abstract

The influence of temperature on the nitrogen removal efficiency of a solid-phase denitrification (SPD) system with a denitrifying SL-205 strain as a bio-augmentation microorganism was investigated in this study. The nitrogen removal effectiveness of the bio-augmentation SPD system declined to 56% when the temperature dropped from 30 to 13 °C. A high-throughput sequencing and correlation analysis revealed that the most dominant genera were Diaphorobacter, Dechloromonas, Clostridium sensu stricto 7, Stenotrophomonas, Thermomomas, Cloacibacterium, Azonexus, and Thauera. Except for Stenotrophomonas, the majority of the prominent bacteria were significantly reduced at lower temperatures. Acidovrax, Azospira, and Cloacibacterium showed a substantial negative correlation with Diaphorobacter in terms of relative abundance. The findings of this study imply that the abundance of the bio-augmented genus diminishes with decreasing temperature, although it remains the most abundant denitrification genus. In addition, given the appropriate hydraulic retention time, a bio-augmentation denitrification system can effectively treat wastewater at low temperatures. These findings can be used to estimate the SPD design parameters and determine the optimal operation approach. These findings provide a theoretical foundation for the bio-augmentation of SPD systems operating at low temperatures.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (32201427); Basic Science (Natural Science) Research Project of Jiangsu Higher Education Institution (22KJD61005); Jiangsu Province Industry-University Research Project (BY2022441); the Key Laboratory Open Projects of Yancheng Teachers University (JKLBS2019009), (JKLBZ2020004); Beijing Natural Science Foundation (Grant No. 8152016); the Special Program for Innovation Ability of Science and Technology of Beijing Academy of agriculture and Forestry Sciences (KJCX20161502-3). We would also like to thank Editage (www.editage.cn) for English-language editing during the preparation of this manuscript.

Funding

The National Natural Science Foundation of China (32201427); Basic Science (Natural Science) Research Project of Jiangsu Higher Education Institution (22KJD61005); Jiangsu Province Industry-University Research Project (BY2022441); the Key Laboratory Open Projects of Yancheng Teachers University (JKLBS2019009, JKLBZ2020004); Beijing Natural Science Foundation (Grant No. 8152016); the Special Program for Innovation Ability of Science and Technology of Beijing Academy of agriculture and Forestry Sciences (KJCX20161502-3).

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Authors and Affiliations

  1. College of Ocean and Bioengineering, Jiangsu Key Laboratory for Bioresources of Saline Soil, Yancheng Teachers University, Tinighu District, Yancheng, 224007, China

    S. Zhang, W. Shen, Y. He & T. Qiu

  2. Jiangsu Key Laboratory for Bioresources of Saline Soil, Yancheng, 224007, China

    S. Zhang & P. Xu

  3. Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng, 224007, China

    S. Zhang & P. Xu

Authors
  1. S. Zhang
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  2. W. Shen
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  3. Y. He
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  4. T. Qiu
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  5. P. Xu
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Contributions

SZ contributed to supervision, writing—reviewing and editing. WS contributed to conceptualization, methodology, writing, YH contributed to data curation visualisation. TQ contributed to investigation. PX contributed to supervision; manuscript revision.

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Correspondence to S. Zhang.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Editorial responsibility: Q. Aguilar-Virgen.

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Zhang, S., Shen, W., He, Y. et al. Efficiency and microbial diversity of PHBV-supported solid-phase denitrification system bio-augmented with Diaphorobacter polyhydroxybutyrativorans bacteria at low temperatures. Int. J. Environ. Sci. Technol. 20, 12285–12294 (2023). https://doi.org/10.1007/s13762-023-04819-7

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  • DOI: https://doi.org/10.1007/s13762-023-04819-7

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