Abstract
The enzyme-assisted biodegradation is considered the efficient and eco-friendly method for the removal of polycyclic aromatic hydrocarbons (PAHs). In this study, immobilized lignin peroxidase (LiP) was prepared by using chitosan-modified halloysite nanotubes (HNTs-CTA) as supports, and the optimum immobilization conditions, stability, and reusability of the immobilized LiP were investigated. The removal efficiencies of PAHs in contaminated soils by the immobilized LiP was evaluated. The results showed that the desired enzyme activity and immobilization efficiency were obtained when the enzyme dosage was 8 mg and the immobilization time was 8 h. The immobilized LiP had higher pH resistance, thermal stability, and storage stability than the free LiP and retained more than 40% of its initial enzyme activity after eight cycles. LiP immobilized on HNTs-CTA was capable of degrading PAHs in soil and had the highest removal efficiency of phenanthrene (55.9%) and fluoranthene (41.2%) in spiked soil after 4 d. The removal efficiency of 16 PAHs in aged soil by the immobilized LiP was 21.1%, and that of low-molecular-weight PAHs was higher than that of medium- and high-molecular-weight PAHs. The removal of PAHs by the immobilized LiP resulted primarily from enzymatic degradation, especially for the removal of medium- and high-molecular-weight PAHs. Consequently, the LiP immobilized on HNTs-CTA has potential applications for the remediation of PAH-contaminated soils.
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This work was supported by the Major Scientific and Technological Innovation Project in Shandong Province (2021CXGC010801) and Shandong Provincial Natural Science Foundation (ZR2020MD107).
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Chai, C., Ji, Y., Wang, N. et al. Immobilized lignin peroxidase on chitosan-modified halloysite nanotubes for degradation of polycyclic aromatic hydrocarbons in soil. Int. J. Environ. Sci. Technol. 20, 10877–10886 (2023). https://doi.org/10.1007/s13762-023-04800-4
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DOI: https://doi.org/10.1007/s13762-023-04800-4