Abstract
Biosurfactants are surface active, amphiphilic, multifunctional, non-toxic and biodegradable alternatives to chemical surfactants. The present study reported biosurfactant production from yeast Debaryomyces hansenii CBS767 using soybean oil as the low-cost carbon source. Characterization of extracellularly synthesized biosurfactant revealed it as of glycolipid type. D. hansenii was able to synthesize the biosurfactant at diverse physiochemical conditions of temperature, pH, percentage of soyabean oil and incubation period. Isolated cell free extract displayed reducing activity as evident by synthesis of silver nanoparticles of size between 23.7 and 77.5 nm. This reducing action might be due to the presence of certain reducing metabolites in cell extract. Silver nanoparticles displayed distinct spherical morphology. This implies surface functionality of the biosurfactant as capping agent helping in regulating the process of nanoparticle aggregation. To aid the future optimization of biosurfactant production from D. hansenii, five support vector machine models were developed, trained and evaluated utilizing experimental optimization datasets. Significant prediction of E24, oil displacement and surface tension using these models encourage the use of machine learning in the future assessment of biosurfactant production.
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Accorsini FR, Mutton MJR, Lemos EGM, Benincasa M (2012) Biosurfactants production by yeasts using soybean oil and glycerol as low cost substrate. Braz J Microbiol 43:116–125. https://doi.org/10.1590/S1517-83822012000100013
Al-Qaysi SAS, Al-Haideri H, Thabit ZA et al (2017) Production, characterization, and antimicrobial activity of mycocin produced by Debaryomyces hansenii DSMZ70238. Int J Microbiol 2017:1–9. https://doi.org/10.1155/2017/2605382
Amaral PFF, da Silva JM, Lehocky M et al (2006) Production and characterization of a bioemulsifier from Yarrowia lipolytica. Process Biochem 41:1894–1898. https://doi.org/10.1016/j.procbio.2006.03.029
Banat IM, Franzetti A, Gandolfi I et al (2010) Microbial biosurfactants production, applications and future potential. Appl Microbiol Biotechnol 87:427–444. https://doi.org/10.1007/s00253-010-2589-0
Banat IM, Makkar RS, Cameotra SS (2000) Potential commercial applications of microbial surfactants. Appl Microbiol Biotechnol 53:495–508
Banat IM, Samarah N, Murad M et al (1991) Biosurfactant production and use in oil tank clean-up. World J Microbiol Biotechnol 7:80–88. https://doi.org/10.1007/BF02310921
Bednarski W, Adamczak M, Tomasik J, Płaszczyk M (2004) Application of oil refinery waste in the biosynthesis of glycolipids by yeast. Bioresour Technol 95:15–18. https://doi.org/10.1016/j.biortech.2004.01.009
Cavalero DA, Cooper DG (2003) The effect of medium composition on the structure and physical state of sophorolipids produced by Candida bombicola ATCC 22214. J Biotechnol 103:31–41
Chandran P, Das N (2010) Biosurfactant production and diesel oil degradation by yeast species Trichosporon asahii isolated from petroleum hydrocarbon contaminated soil. Int J Eng Sci Technol 2:6942
Cooper DG (1986) Biosurfactants. Microbiol Sci 3:145–149
Cooper DG, Goldenberg BG (1987) Surface-active agents from two bacillus species. Appl Environ Microbiol 53:224–229
Cooper DG, Paddock DA (1984) Production of a Biosurfactant from Torulopsis bombicola. Appl Environ Microbiol 47:173–176
Cortes-Sánchez Alejandro C-S, Humberto H-S, Maria J-F (2011) Production of glycolipids with antimicrobial activity by Ustilago maydis FBD12 in submerged culture. Afr J Microbiol Res 5:2512–2523. https://doi.org/10.5897/AJMR10.814
De Carvalho CCCR, Fernandes P (2010) Production of metabolites as bacterial responses to the marine environment. Mar Drugs 8:705–727
Deleu M, Paquot M (2004) From renewable vegetables resources to microorganisms: new trends in surfactants. C R Chim 7:641–646. https://doi.org/10.1016/j.crci.2004.04.002
Desai JD, Banat IM (1997) Microbial production of surfactants and their commercial potential. Microbiol Mol Biol Rev 61:47–64
Dilmohamud BA, Seeneevassen J, Rughooputh SDDV, Ramasami P (2005) Surface tension and related thermodynamic parameters of alcohols using the Traube stalagmometer. Eur J Phys 26:1079–1084. https://doi.org/10.1088/0143-0807/26/6/015
dos Reis CBL, Morandini LMB, Bevilacqua CB et al (2018) First report of the production of a potent biosurfactant with α, β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation. Braz J Microbiol 49:185–192. https://doi.org/10.1016/J.BJM.2018.04.004
Drucker H, Drucker H, Burges CJC et al (1997) Support vector regression machines. Adv Neural Inf Process Syst 9(9):155–161
Edwards KR, Lepo JE, Lewis MA (2003) Toxicity comparison of biosurfactants and synthetic surfactants used in oil spill remediation to two estuarine species. Mar Pollut Bull 46:1309–1316. https://doi.org/10.1016/S0025-326X(03)00238-8
Elazzazy AM, Abdelmoneim TS, Almaghrabi OA (2015) Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia. Saudi J Biol Sci 22:466–475. https://doi.org/10.1016/J.SJBS.2014.11.018
Elshafie AE, Joshi SJ, Al-Wahaibi YM et al (2015) Sophorolipids production by Candida bombicola ATCC 22214 and its potential application in microbial enhanced oil recovery. Front Microbiol 6:1324. https://doi.org/10.3389/fmicb.2015.01324
Eswari JS, Dhagat S, Mishra P (2018) Biosurfactant assisted silver nanoparticle synthesis: a critical analysis of its drug design aspects. Adv Nat Sci Nanosci Nanotechnol 9:045007. https://doi.org/10.1088/2043-6254/aaec0e
Farias CBB, Silva AF, Rufino RD et al (2014) Synthesis of silver nanoparticles using a biosurfactant produced in low-costmediumas stabilizing agent. Electron J Biotechnol 17:122–125. https://doi.org/10.1016/j.ejbt.2014.04.003
Fiechter A (1992) Biosurfactants: moving towards industrial application. Trends Biotechnol 10:208–217
Georgiou G, Lin S-C, Sharma MM (1992) Surface-active compounds from microorganisms. Nat Biotechnol 10:60–65. https://doi.org/10.1038/nbt0192-60
Goebbert U, Lang S, Wagner F (1984) Biotechnology letters. Kluwer/Plenum Publishers
Guerra-Santos L, Kappeli O, Fiechter A (1986) Dependence of Pseudomonas aeruginosa continous culture biosurfactant production on nutritional and environmental factors. Appl Microbiol Biotechnol. https://doi.org/10.1007/BF00250320
Gupta R (1994a) Emulsifying activity of hydrocarbonoclastic marine yeasts
Hemlata B, Selvin J, Tukaram K (2015) Optimization of iron chelating biosurfactant production by Stenotrophomonas maltophilia NBS-11. Biocatal Agric Biotechnol 4:135–143. https://doi.org/10.1016/J.BCAB.2015.02.002
Ilori MO, Amobi CJ, Odocha AC (2005) Factors affecting biosurfactant production by oil degrading Aeromonas spp. isolated from a tropical environment. Chemosphere 61:985–992. https://doi.org/10.1016/j.chemosphere.2005.03.066
Jain DK, Collins-Thompson DL, Lee H, Trevors JT (1991) A drop-collapsing test for screening surfactant-producing microorganisms. J Microbiol Methods 13:271–279. https://doi.org/10.1016/0167-7012(91)90064-W
Jimoh AA, Lin J (2020) Biotechnological applications of Paenibacillus sp. D9 Lipopeptide biosurfactant produced in low-cost substrates. Appl Biochem Biotechnol 191:921–941. https://doi.org/10.1007/s12010-020-03246-5
Joshi PA, Shekhawat DB (2014) Screening and isolation of biosurfactant producing bacteria from petroleum contaminated soil. Eur J Exp Biol 4:164
Kasture MB, Patel P, Prabhune AA et al (2008) Synthesis of silver nanoparticles by sophorolipids: effect of temperature and sophorolipid structure on the size of particles. J Chem Sci 120(6):515–520
Kiran GS, Hema TA, Gandhimathi R et al (2009) Optimization and production of a biosurfactant from the sponge-associated marine fungus Aspergillus ustus MSF3. Colloids Surf B Biointerfaces 73:250–256. https://doi.org/10.1016/j.colsurfb.2009.05.025
Kiran GS, Sabu A, Selvin J (2010) Synthesis of silver nanoparticles by glycolipid biosurfactant produced from marine Brevibacterium casei MSA19. J Biotechnol 148:221–225. https://doi.org/10.1016/j.jbiotec.2010.06.012
Konishi M, Fukuoka T, Morita T et al (2008) Production of new types of sophorolipids by Candida batistae. J Oleo Sci 57:359–369
Korayem AS, Abdelhafez AA, Zaki MM, Saleh EA (2015) Optimization of biosurfactant production by Streptomyces isolated from Egyptian arid soil using Plackett-Burman design. Ann Agric Sci 60:209–217. https://doi.org/10.1016/J.AOAS.2015.09.001
Lai CC, Huang YC, Wei YH, Chang JS (2009) Biosurfactant-enhanced removal of total petroleum hydrocarbons from contaminated soil. J Hazard Mater 167:609–614. https://doi.org/10.1016/j.jhazmat.2009.01.017
Lambert JB (1998) Organic structural spectroscopy. Prentice Hall
Lima FA, Santos OS, Pomella AWV et al (2020) Culture medium evaluation using low-cost substrate for biosurfactants lipopeptides production by Bacillus amyloliquefaciens in pilot bioreactor. J Surfactants Deterg 23:91–98. https://doi.org/10.1002/jsde.12350
Lima RA, Andrade RFS, Rodríguez DM et al (2017) Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates. Afr J Microbiol Res 11:237–244. https://doi.org/10.5897/AJMR2016.8341
Lin S-C (1996) Review biosurfactants: recent advances. J Chem Technol Biotechnol 66:109–120. https://doi.org/10.1002/(SICI)1097-4660(199606)66:2%3c109::AID-JCTB477%3e3.0.CO;2-2
López-Linares JC, Romero I, Cara C et al (2018) Xylitol production by Debaryomyces hansenii and Candida guilliermondii from rapeseed straw hemicellulosic hydrolysate. Bioresour Technol 247:736–743. https://doi.org/10.1016/J.BIORTECH.2017.09.139
Luna JM, Rufino RD, Albuquerque CDC et al (2011) Economic optimized medium for tensio-active agent production by Candida sphaerica UCP0995 and application in the removal of hydrophobic contaminant from sand. Int J Mol Sci 12:2463–2476. https://doi.org/10.3390/ijms12042463
Makkar RS, Rockne KJ (2003) Comparison of synthetic surfactants and biosurfactants in enhancing biodegradation of polycyclic aromatic hydrocarbons. Environ Toxicol Chem 22:2280–2292
Menon V, Prakash G, Rao M (2010) Enzymatic hydrolysis and ethanol production using xyloglucanase and Debaromyces hansenii from tamarind kernel powder: galactoxyloglucan predominant hemicellulose. J Biotechnol 148:233–239. https://doi.org/10.1016/j.jbiotec.2010.06.004
Minhas A, Biswas D, Mondal AK (2009) Development of host and vector for high-efficiency transformation and gene disruption in Debaryomyces hansenii. FEMS Yeast Res 9:95–102. https://doi.org/10.1111/j.1567-1364.2008.00457.x
Minhas A, Sharma A, Kaur H et al (2012) Conserved Ser/Arg-rich Motif in PPZ Orthologs from fungi is important for its role in cation tolerance. J Biol Chem 287:7301–7312. https://doi.org/10.1074/jbc.M111.299438
Mukherjee S, Das P, Sen R (2006) Towards commercial production of microbial surfactants. Trends Biotechnol 24:509–515. https://doi.org/10.1016/j.tibtech.2006.09.005
Naumann D (2006) Infrared spectroscopy in microbiology. Encyclopedia of analytical chemistry. Wiley, Chichester
Nwaguma IV, Chikere CB, Okpokwasili GC (2016) Isolation, characterization, and application of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland. Nigeria Bioresour Bioprocess 3:40. https://doi.org/10.1186/s40643-016-0118-4
Olivera NL, Commendatore MG, Delgado O, Esteves JL (2003) Microbial characterization and hydrocarbon biodegradation potential of natural bilge waste microflora. J Ind Microbiol Biotechnol 30:542–548. https://doi.org/10.1007/s10295-003-0078-5
Pal S, Choudhary V, Kumar A et al (2013) Studies on xylitol production by metabolic pathway engineered Debaryomyces hansenii. Bioresour Technol 147:449–455. https://doi.org/10.1016/j.biortech.2013.08.065
Palanisamy P, Raichur AM (2009) Synthesis of spherical NiO nanoparticles through a novel biosurfactant mediated emulsion technique. Mater Sci Eng C 29:199–204. https://doi.org/10.1016/j.msec.2008.06.008
Perfumo A, Rancich I, Banat IM (2010) Possibilities and challenges for biosurfactants use in petroleum industry. Adv Exp Med Biol 672:135–145. https://doi.org/10.1007/978-1-4419-5979-9_10
Pontil M, Rifkin RM, Evgeniou T (1999) From regression to classification in support vector machines. Undefined
Portilla OM, Rivas B, Torrado A et al (2008) Revalorisation of vine trimming wastes using Lactobacillus acidophilus and Debaryomyces hansenii. J Sci Food Agric 88:2298–2308. https://doi.org/10.1002/jsfa.3351
Prince RC (1997) Bioremediation of marine oil spills. Trends Biotechnol 15:158–160. https://doi.org/10.1016/S0167-7799(97)01033-0
Rahman PKSM, Gakpe E (2008) Production, Characterisation and applications of biosurfactants-review. Biotechnology 7:360–370. https://doi.org/10.3923/biotech.2008.360.370
Ramana KV, Karanth NG (2007) Factors affecting biosurfactant production using Pseudomonas aeruginosa CFTR-6 under submerged conditions. J Chem Technol Biotechnol 45:249–257. https://doi.org/10.1002/jctb.280450402
Reddy AS, Chen C-Y, Chen C-C et al (2009a) Synthesis of gold nanoparticles via an environmentally benign route using a biosurfactant. J Nanosci Nanotechnol 9:6693–6699
Reddy AS, Chen CY, Baker SC et al (2009b) Synthesis of silver nanoparticles using surfactin: a biosurfactant as stabilizing agent. Mater Lett 63:1227–1230. https://doi.org/10.1016/j.matlet.2009.02.028
Reznik GO, Vishwanath P, Pynn MA et al (2010) Use of sustainable chemistry to produce an acyl amino acid surfactant. Appl Microbiol Biotechnol 86:1387–1397. https://doi.org/10.1007/s00253-009-2431-8
Rufino RD, de Luna JM, de Campos Takaki GM, Sarubbo LA (2014) Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988. Electron J Biotechnol 17:34–38. https://doi.org/10.1016/J.EJBT.2013.12.006
Santos DKF, Rufino RD, Luna JM et al (2016) Biosurfactants: multifunctional biomolecules of the 21st century. Int J Mol Sci 17:401
Saravanan V, Vijayakumar S (2012) Isolation and screening of biosurfactant producing microorganisms. J Acad Ind Res 1:1–5
Sarubbo L (2014) Biosurfactants: classification, properties and environmental applications. Recent Dev Biotechnol 11:1–29
Sarubbo LA, Luna JM, Rufino RD (2015) Application of a biosurfactant produced in low-cost substrates in the removal of hydrophobic contaminants. Chem Eng Trans 43:295–300. https://doi.org/10.3303/CET1543050
Satpute SK, Banat IM, Dhakephalkar PK et al (2010) Biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms. Biotechnol Adv 28:436–450
Sharma D, Saharan BS (2016) Functional characterization of biomedical potential of biosurfactant produced by Lactobacillus helveticus. Biotechnol Rep 11:27–35. https://doi.org/10.1016/J.BTRE.2016.05.001
Sharma D, Saharan BS, Chauhan N et al (2015) Isolation and functional characterization of novel biosurfactant produced by Enterococcus faecium. SpringerPlus 4:4. https://doi.org/10.1186/2193-1801-4-4
Shekhar S, Sundaramanickam A, Balasubramanian T (2015) Biosurfactant producing microbes and their potential applications: a review. Crit Rev Environ Sci Technol 45:1522–1554. https://doi.org/10.1080/10643389.2014.955631
Singh A, Van Hamme JD, Ward OP (2007) Surfactants in microbiology and biotechnology: part 2. Appl Aspects Biotechnol Adv 25:99–121. https://doi.org/10.1016/j.biotechadv.2006.10.004
Singh M, Desai JD (1989) Hydrocarbon emulsification by Candida tropicalis and Debaryomyces polymorphus. Indian J Exp Biol 27:224–226
Singh P, Tiwary BN (2016) Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India. Bioresour Bioprocess 3:42. https://doi.org/10.1186/s40643-016-0119-3
Solaiman DKY, Ashby RD, Nuñez A, Foglia TA (2004) Production of sophorolipids by Candida bombicola grown on soy molasses as substrate. Biotechnol Lett 26:1241–1245. https://doi.org/10.1023/B:BILE.0000036605.80577.30
Tripathi L, Irorere VU, Marchant R, Banat IM (2018) Marine derived biosurfactants: a vast potential future resource. Biotechnol Lett 40:1441–1457
Vater J (2007) Lipopetides, an attractive class of microbial surfactants. Polymers as colloid systems. Steinkopff, pp 12–18
Viana RB, da Silva ABF, Pimentel AS (2012) Infrared Spectroscopy of anionic, cationic, and zwitterionic surfactants. Adv Phys Chem 2012:1–14. https://doi.org/10.1155/2012/903272
Xie Y, Ye R, Liu H (2006) Synthesis of silver nanoparticles in reverse micelles stabilized by natural biosurfactant. Colloids Surf A Physicochem Eng Asp 279:175–178. https://doi.org/10.1016/J.COLSURFA.2005.12.056
Yang C, Mills D, Mathee K et al (2006) An ecoinformatics tool for microbial community studies: supervised classification of Amplicon Length Heterogeneity (ALH) profiles of 16S rRNA. J Microbiol Methods 65:49–62. https://doi.org/10.1016/j.mimet.2005.06.012
Yin H, Qiang J, Jia Y et al (2009) Characteristics of biosurfactant produced by Pseudomonas aeruginosa S6 isolated from oil-containing wastewater. Process Biochem 44:302–308. https://doi.org/10.1016/J.PROCBIO.2008.11.003
Youssef NH, Duncan KE, Nagle DP et al (2004) Comparison of methods to detect biosurfactant production by diverse microorganisms. J Microbiol Methods 56:339–347. https://doi.org/10.1016/j.mimet.2003.11.001
Acknowledgements
A.P.M. acknowledges the Director, UIET for providing facilities for research. Special thanks to Dr. N. Gupta for technical assistance.
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This study was partial financially supported by Technical Education Quality Improvement Programme (TEQIP) assisted by World Bank project.
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Conceptualization: APM Methodology: APM, MK, Formal analysis and investigation: JK, SK, PK, Writing—original draft preparation: APM, SK, MK; Writing—review and editing: APM, MK, JK, PK Funding acquisition: APM, JK Resources: APM, JK Supervision: APM, MK, JK.
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Kaur, J., Kaur, S., Kumar, M. et al. Studies on production, optimization and machine learning-based prediction of biosurfactant from Debaryomyces hansenii CBS767. Int. J. Environ. Sci. Technol. 19, 8465–8478 (2022). https://doi.org/10.1007/s13762-021-03639-x
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DOI: https://doi.org/10.1007/s13762-021-03639-x