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Scale-up treatment of petroleum hydrocarbon-contaminated soil using a defined microbial consortium


Bioremediation strategies apply environmental microbes to metabolize organic compounds and can be useful for the treatment of oil-contaminated soils. In this study, different approaches of bioremediation were compared on a scale-up treatment. The defined microbial consortium was formulated with degrading microorganisms previously selected (Pseudomonas mendoncina BPB 1.8, Bacillus cereus BPB 1.20, Bacillus cereus BPB 1.26, and Bacillus sphaericus BPB 1.35). Bioaugmentation/biostimulation, biostimulation, and natural attenuation strategies were evaluated after 60 days of treatment by gas chromatography. The contaminant level remained elevated after the treatments using natural attenuation and biostimulation. However, the bioaugmentation with biostimulation treatment showed a satisfactory ability to degrade petroleum hydrocarbons (85%). Interestingly, no correlation was observed with the presence of hydrocarbon-degrading microorganisms and CO2 production, and denaturing gradient gel electrophoresis exhibited no significant difference in the biodiversity of the treatments. Although, the results showed that the microbial consortium was imperative to the successful biodegradation of TPH-contaminated gas station soil.

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The authors would like to thank Bioplus Desenvolvimento Biotecnológico Ltda for the use of facilities and financial support. This work was financially supported through grants from the following Brazilian agencies: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES). MHV have been supported by Grant Numbers [302637/2017-6] and [307191/2016-8], respectively.

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All authors have contributed to the writing of this manuscript. All authors have given approval to the final version of the manuscript.

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Correspondence to A. P. Napp.

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The authors declare that they have no conflict of interest.

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Editorial responsibility: BV Thomas.

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Napp, A.P., Allebrandt, S.R., Pereira, J.E.S. et al. Scale-up treatment of petroleum hydrocarbon-contaminated soil using a defined microbial consortium. Int. J. Environ. Sci. Technol. 19, 6023–6032 (2022).

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  • Bioaugmentation
  • Bioremediation
  • Biostimulation
  • Gas station
  • TPH-contaminated soils