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Characterization and antioxidant-antimicrobial activity of silver nanoparticles synthesized using Punica granatum extract

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Abstract

In this study, low-cost, simple, and eco-friendly green synthesis of silver nanoparticles was performed using pomegranate extract (Ag NPs/PG). Punica granatum extract acts as a reducing agent and capping agent in the synthesis of Ag NPs. The presence of Ag NPs/PG was analyzed using characterization methods such as UV–Vis absorption spectroscopy (UV–Vis), Fourier transform infrared spectrophotometer (FTIR), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and transmission electron microscopy (TEM). XRD, TEM, and XPS analysis showed the presence elements, surface morphology, various shapes, and the presence of Ag NPs in the sample. The Ag NPs/PG particle sizes were found to be 5–45 nm in TEM analysis. By using the XRD technique, Ag NPs were determined to be a cubic crystal structure with face-centered. Furthermore, the antioxidant and antibacterial properties of Ag NPs/PG were also investigated. The enhanced biological activity of Punica granatum-mediated green synthesized Ag NPs was investigated based on antioxidant, antimicrobial, and DNA cleavage studies. The Ag NPs showed 91.6% DPPH (2,2-diphenyl-1-picrylhydrazyl) radical inhibition rate activity at the highest concentration (500 mg mL−1). It exhibited significant chemical nuclease activity. Ag NPs/PG had significant antibacterial activity against gram-positive and gram-negative bacteria, but gram-negative bacteria more susceptible than gram-positive. Biogenic synthesis of Ag NPs with the help of Punica granatum extract is a useful and environmentally safe synthesis technique. It is thought that Ag NPs obtained using Punica granatum may have potential use in the fields of nanomedicine, nanobiosensor, and nanobiotechnology in the near future.

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The authors declare that there are no competing interests.

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Authors and Affiliations

  1. Sen Research Group, Biochemistry Department, Faculty of Arts and Science, Dumlupinar University, Evliya Celebi Campus, 43100, Kutahya, Turkey

    A. Aygün & F. Şen

  2. Food Processing Programme, Technical Science Vocational School, Mersin University, Yenisehir, TR-33343, Mersin, Turkey

    S. Özdemir

  3. Chemistry Department, Faculty of Science, Van Yüzüncü Yıl University, Zeve Campus, 65080, Van, Turkey

    M. Gülcan

  4. Deparment of Chemical and Chemical Processing Technologies, Technical Science Vocational School, Mersin University, Yenisehir, TR-33343, Mersin, Turkey

    M. S. Yalçın

  5. Chemistry Department, Faculty of Arts and Science, Afyon Kocatepe University, 03200, Afyon, Turkey

    M. Uçar

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  1. A. Aygün
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  2. S. Özdemir
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  4. M. S. Yalçın
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  6. F. Şen
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Contributions

AA, SÖ, MG, MSY, MU, and FS participated in the study design and coordination, conducted experimental studies, and prepared the manuscript. All authors have read and approved the last article.

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Correspondence to F. Şen.

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Funding

LB is supported by a project [SR/WOS-A/LS-45/2018 (G)] granted by the Department of Science and Technology, Government of India under the DST Women Scientist Scheme.

Acknowledgements

Authors thank Bose Institute for the necessary facilities. We acknowledge the technical assistance of Ms. Tanima Modak Dhar at Bose Institute in handling the Atomic Absorption Spectrophotometer.

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Editorial responsibility: Samareh Mirkia.

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Aygün, A., Özdemir, S., Gülcan, M. et al. Characterization and antioxidant-antimicrobial activity of silver nanoparticles synthesized using Punica granatum extract. Int. J. Environ. Sci. Technol. 19, 2781–2788 (2022). https://doi.org/10.1007/s13762-021-03246-w

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  • DOI: https://doi.org/10.1007/s13762-021-03246-w

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