Biosorption of xenobiotic Reactive Black B onto metabolically inactive T. harzianum biomass: optimization and equilibrium studies

  • V. Karthik
  • N. Sivarajasekar
  • V. C. Padmanaban
  • E. Nakkeeran
  • N. SelvarajuEmail author
Original Paper


Biosorptive removal of Reactive Black B (RBB) from aqueous solution by metabolically inactive Trichoderma harzianum (T. harzianum) was examined in this study. The individual and interactive effects of pH (2.0–10.0), temperature (25–45 °C), initial dye concentration (100–300 mg/L) and adsorbent dosage (0.1–0.5 mg/L) on the biosorption of RBB were evaluated using central composite design (CCD), and the mathematical model describing the biosorption process was developed. Maximum removal percentage (97.42 ± 1.3%) of RBB was attained at pH 5.75, temperature 37.12 °C, initial dye concentration 210.92 mg/L and biosorbent dosage 0.28 g/L. Suitability of the isotherms were determined using Langmuir, Freundlich and Temkin in this study, among which Freundlich isotherm was found suitable to equilibrium data. Kinetic analysis revealed that the adsorption data correlated well with pseudo-second-order kinetic model. The obtained thermodynamic data revealed that the RBB adsorption onto T. harzianum was exothermic and spontaneous nature during the process.


Adsorption Central-composite design Isotherm Kinetic Thermodynamic 



Many thanks to Centre for Environmental studies, Government College of Technology, Coimbatore, Tamilnadu, India, for providing the laboratory facilities to undertake this research work.


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Copyright information

© Islamic Azad University (IAU) 2018

Authors and Affiliations

  • V. Karthik
    • 1
  • N. Sivarajasekar
    • 2
  • V. C. Padmanaban
    • 3
  • E. Nakkeeran
    • 4
  • N. Selvaraju
    • 5
    Email author
  1. 1.Department of Industrial BiotechnologyGovernment College of TechnologyCoimbatoreIndia
  2. 2.Laboratory for Bioremediation Research, Unit Operations Laboratory, Department of BiotechnologyKumaraguru College of TechnologyCoimbatoreIndia
  3. 3.Department of BiotechnologyKamaraj College of Engineering and TechnologyVirudhunagarIndia
  4. 4.Department of Chemical EngineeringNational Institute of TechnologyCalicutIndia
  5. 5.Department of Biosciences and BioengineeringIndian Institute of Technology GuwahatiGuwahatiIndia

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