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High-performing antifouling bacterial consortium for submerged membrane bioreactor treating synthetic wastewater

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Abstract

Biofouling, associated with membranes, is considered as a major operational challenge in membrane bioreactor (MBR) technology. Interrupting the process for the formation of biofilm by the action of interspecies quorum quenching (QQ) has received a significant attention since recent years. An antifouling bacterial consortium was identified to improve biofouling inhibition performance during MBR operation. For this purpose, various QQ bacteria were isolated from laboratory-scale MBR using enrichment culture method and identified via 16S rRNA. Potential quenching strains including Enterobacter cloaca, Delftia sp., and Pseudomonas sp. were utilized to control biofouling in the MBR operated in the continuous mode for 38 days. Three laboratory-scale MBRs, including two MBRs with different anti fouling consortium and a control, were operated in parallel under similar operating conditions. Biofouling control by QQ bacteria was compared based on the membrane permeability and EPS secretion from biofilm on the membrane. Both MBRs with antifouling consortium (AC-MBRs) experienced around three times less biofouling as compared to conventional MBR leading to significant decrease in acyl homoserine lactones (AHLs) concentration in the biocake. More than 90, 45, and 49% of COD, NH4–N, and PO4 3−–P removal efficiencies elucidate that QQ bacterial consortium could effectively reduce membrane biofouling without compromising the MBR efficiency. Comparatively lower concentration of bound EPS in AC-MBRs restricted the bacterial adhesion to membrane resulting in enhanced membrane permeability depicting that a broader range of signal molecules could be hydrolyzed using antifouling consortium than single or no QQ strain in the submerged MBR.

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References

  • American Public Health Association (APHA), American Water Works Association (AWWA), Water Environment Federation (WEF) (2012) Standard methods for the examination of water and wastewater, 21st edn. Washington

  • Basuvaraj M, Fein J, Liss SN (2015) Protein and polysaccharide content of tightly and loosely bound extracellular polymeric substances and the development of a granular activated sludge floc. Water Res 82:104–117. doi:10.1016/j.watres.2015.05.014

    Article  CAS  Google Scholar 

  • Cheong W-S, Lee C-H, Moon Y-H et al (2013) Isolation and identification of indigenous quorum quenching bacteria, Pseudomonas sp. 1A1, for biofouling control in MBR. Ind Eng Chem Res 52:10554–10560. doi:10.1021/ie303146f

    Article  CAS  Google Scholar 

  • Choo K-H, Lee C-H (1996) Membrane fouling mechanisms in the membrane-coupled anaerobic bioreactor. Water Res 30:1771–1780. doi:10.1016/0043-1354(96)00053-X

    Article  CAS  Google Scholar 

  • Christiaen SEA, Brackman G, Nelis HJ, Coenye T (2011) Isolation and identification of quorum quenching bacteria from environmental samples. J Microbiol Methods 87:213–219. doi:10.1016/j.mimet.2011.08.002

    Article  CAS  Google Scholar 

  • Drews A (2010) Membrane fouling in membrane bioreactors—characterisation, contradictions, cause and cures. J Membr Sci 363:1–28

    Article  CAS  Google Scholar 

  • DuBois M, Gilles KA, Hamilton JK et al (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356. doi:10.1021/ac60111a017

    Article  CAS  Google Scholar 

  • Frølund B, Palmgren R, Keiding K, Nielsen PH (1996) Extraction of extracellular polymers from activated sludge using a cation exchange resin. Water Res 30:1749–1758. doi:10.1016/0043-1354(95)00323-1

    Article  Google Scholar 

  • Gao D-W, Wen Z-D, Li B, Liang H (2013) Membrane fouling related to microbial community and extracellular polymeric substances at different temperatures. Bioresour Technol 143:172–177. doi:10.1016/j.biortech.2013.05.127

    Article  CAS  Google Scholar 

  • Huang JJ, Han J-I, Zhang L-H, Leadbetter JR (2003) Utilization of acyl-homoserine lactone quorum signals for growth by a soil pseudomonad and Pseudomonas aeruginosa PAO1. Appl Environ Microbiol 69:5941–5949. doi:10.1128/AEM.69.10.5941-5949.2003

    Article  CAS  Google Scholar 

  • Hwang B-K, Lee C-H, Chang I-S et al (2012) Membrane bioreactor: TMP rise and characterization of bio-cake structure using CLSM-image analysis. J Membr Sci 419–420:33–41. doi:10.1016/j.memsci.2012.06.031

    Article  Google Scholar 

  • Kim A-L, Park S-Y, Lee C-H et al (2014) Quorum quenching bacteria isolated from the sludge of a wastewater treatment plant and their application for controlling biofilm formation. J Microbiol Biotechnol 24:1574–1582

    Article  CAS  Google Scholar 

  • Kim S-R, Lee K-B, Kim J-E et al (2015) Macroencapsulation of quorum quenching bacteria by polymeric membrane layer and its application to MBR for biofouling control. J Membr Sci 473:109–117. doi:10.1016/j.memsci.2014.09.009

    Article  CAS  Google Scholar 

  • Lee B, Yeon K-M, Shim J et al (2014) Effective antifouling using quorum-quenching acylase stabilized in magnetically-separable mesoporous silica. Biomacromol 15:1153–1159. doi:10.1021/bm401595q

    Article  CAS  Google Scholar 

  • Lin H, Zhang M, Wang F et al (2014) A critical review of extracellular polymeric substances (EPSs) in membrane bioreactors: characteristics, roles in membrane fouling and control strategies. J Membr Sci. doi:10.1016/j.memsci.2014.02.034

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Maqbool T, Khan SJ, Waheed H et al (2015) Membrane biofouling retardation and improved sludge characteristics using quorum quenching bacteria in submerged membrane bioreactor. J Membr Sci 483:75–83. doi:10.1016/j.memsci.2015.02.011

    Article  CAS  Google Scholar 

  • Meng F, Chae S-R, Drews A et al (2009) Recent advances in membrane bioreactors (MBRs): membrane fouling and membrane material. Water Res 43:1489–1512. doi:10.1016/j.watres.2008.12.044

    Article  CAS  Google Scholar 

  • Metzger U, Le-Clech P, Stuetz RM et al (2007) Characterisation of polymeric fouling in membrane bioreactors and the effect of different filtration modes. J Membr Sci 301:180–189. doi:10.1016/j.memsci.2007.06.016

    Article  CAS  Google Scholar 

  • Rasmussen TB, Bjarnsholt T, Skindersoe ME et al (2005) Screening for quorum-sensing inhibitors (QSI) by use of a novel genetic system, the QSI selector. J Bacteriol 187:1799–1814. doi:10.1128/JB.187.5.1799-1814.2005

    Article  CAS  Google Scholar 

  • Shrout JD, Nerenberg R (2012) Monitoring bacterial twitter: does quorum sensing determine the behavior of water and wastewater treatment biofilms? Environ Sci Technol 46:1995–2005. doi:10.1021/es203933h

    Article  CAS  Google Scholar 

  • Siddiqui MF, Sakinah M, Singh L, Zularisam AW (2012) Targeting N-acyl-homoserine-lactones to mitigate membrane biofouling based on quorum sensing using a biofouling reducer. J Biotechnol 161:190–197. doi:10.1016/j.jbiotec.2012.06.029

    Article  CAS  Google Scholar 

  • Siddiqui MF, Rzechowicz M, Harvey W et al (2015) Quorum sensing based membrane biofouling control for water treatment: a review. J Water Process Eng 7:112–122. doi:10.1016/j.jwpe.2015.06.003

    Article  Google Scholar 

  • Taga ME, Bassler BL (2003) Chemical communication among bacteria. Proc Natl Acad Sci USA 100(Suppl 2):14549–14554. doi:10.1073/pnas.1934514100

    Article  CAS  Google Scholar 

  • Tsuneda S, Aikawa H, Hayashi H et al (2003) Extracellular polymeric substances responsible for bacterial adhesion onto solid surface. FEMS Microbiol Lett 223:287–292

    Article  CAS  Google Scholar 

  • Waheed H, Hashmi I, Khan SJ et al (2016) Microbial population dynamics and profiling of quorum sensing agents in membrane bioreactor. Int Biodeterior Biodegradation 113:66–73. doi:10.1016/j.ibiod.2015.12.014

    Article  CAS  Google Scholar 

  • Wahjudi M, Papaioannou E, Hendrawati O et al (2011) PA0305 of Pseudomonas aeruginosa is a quorum quenching acylhomoserine lactone acylase belonging to the Ntn hydrolase superfamily. Microbiol Read Engl 157:2042–2055. doi:10.1099/mic.0.043935-0

    Article  CAS  Google Scholar 

  • Wang W-Z, Morohoshi T, Ikenoya M et al (2010) AiiM, a novel class of N-acylhomoserine lactonase from the leaf-associated bacterium Microbacterium testaceum. Appl Environ Microbiol 76:2524–2530. doi:10.1128/AEM.02738-09

    Article  CAS  Google Scholar 

  • Wilén B-M, Jin B, Lant P (2003) The influence of key chemical constituents in activated sludge on surface and flocculating properties. Water Res 37:2127–2139. doi:10.1016/S0043-1354(02)00629-2

    Article  Google Scholar 

  • Zhang J, Chua HC, Zhou J, Fane AG (2006) Factors affecting the membrane performance in submerged membrane bioreactors. J Membr Sci 284:54–66. doi:10.1016/j.memsci.2006.06.022

    Article  CAS  Google Scholar 

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Acknowledgements

Authors acknowledge the Higher Education Commission (21361340-2SS2002) and National University of Sciences and Technology (NUST), Pakistan, for providing the research funds. We would like to thank Dr Xiao Yeyuan, for his help in LCMS analysis, Professor Chung Hak Lee, Seoul National University, South Korea, for providing QQ strain, RBH4, and Dr. Tom Coenye, Ghent University, Belgium, for provision of QSIS2 biosensor.

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Authors and Affiliations

  1. Institute of Environmental Sciences and Engineering, School of Civil and Environmental Engineering, National University of Sciences and Technology, H-12 Sector, Islamabad, Pakistan

    H. Waheed, S. Pervez, I. Hashmi & S. J. Khan

  2. Department of Chemical and Environmental Engineering, School of Engineering and Applied Science, Yale University, New Haven, CT, USA

    S.-R. Kim

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  1. H. Waheed
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  2. S. Pervez
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  3. I. Hashmi
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  4. S. J. Khan
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  5. S.-R. Kim
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Correspondence to I. Hashmi.

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Editorial responsibility: M. Abbaspour.

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Waheed, H., Pervez, S., Hashmi, I. et al. High-performing antifouling bacterial consortium for submerged membrane bioreactor treating synthetic wastewater. Int. J. Environ. Sci. Technol. 15, 395–404 (2018). https://doi.org/10.1007/s13762-017-1392-1

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  • DOI: https://doi.org/10.1007/s13762-017-1392-1

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