Skip to main content
Log in

Potential of fungus Trichoderma harzianum for toxicity reduction in municipal solid waste leachate

  • Short Communication
  • Published:
International Journal of Environmental Science and Technology Aims and scope Submit manuscript

Abstract

The purpose of this research was to test the ability of selected fungus Trichoderma harzianum for municipal solid waste leachate treatment. The research carried out by inoculating the enzyme produced by T. harzianum with different ratio (10% up to 80% v/v) of solid waste leachate. The findings clearly indicated that the enzymatic addition was effectively acted (86.09% of chemical oxygen demand removed), and the rate of chemical degradation amended treatment enhanced as compared to control. Also, the enzymatic addition excellently reduced the inhibition of germination (35.8 ± 0.7) of seed (Zea mays L.) and improved the environmental quality of treated leachate. Therefore, these results can be use to articulate the preliminary feedstock for pilot to field-scale application.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

References

  • Abdullah N, Wan Razarinah WAR, Mahmood NZ, Taha RM (2013) Treatment of landfill leachate using Ganoderma australe mycelia immobilized on Ecomat. Int J Environ Sci Dev 4(5):483–487

    Article  CAS  Google Scholar 

  • Adikane HV, Dange MN, Selvakumari K (2006) Optimization of anaerobically digested distillery molasses spent wash decolorization using soil as inoculum in the absence of additional carbon and nitrogen source. Bioresour Technol 97:2131–2135

    Article  CAS  Google Scholar 

  • Ahmed S, Bashir A, Saleem H, Saadia M, Jamil A (2009) Production and purification of cellulose degrading enzymes from filamentous fungus Trichoderma harzianum. Pak J Bot 41(3):1411–1419

    CAS  Google Scholar 

  • Anastasi A, Tigini V, Varese GC, Goltapeh EM, et al. (eds) (2013) Fungi as bioremediators. Soil Biol 32:29–49. doi:10.1007/978-3-642-33811-3_2. Springer, Berlin

  • APHA (2005) Standard methods for examination water and wastewaters, 21st edn. American Public Health Association, Washington

    Google Scholar 

  • Asgher M, Bashir F, Iqbal HMN (2014) A comprehensive ligninolytic pre-treatment approach from lignocellulose green biotechnology to produce bio-ethanol. Chem Eng Res Des 92:1571–1578

    Article  CAS  Google Scholar 

  • Awasthi AK, Pandey AK, Khan J (2017) An eco-friendly approach for minimizing pollution of metal from municipal solid waste leachate in India. J Clean Prod 140:1618–1625

    Article  CAS  Google Scholar 

  • Baderna D, Maggioni S, Boriani E, Gemma S, Molteni M, Lombardo A, Colombo A, Bordonali S, Rotella G, Lodi M, Benfenati E (2011) A combined approach to investigate the toxicity of an industrial land-fill’s leachate: chemical analyses, risk assessment and in vitro as-says. Environ Res 111:603–613

    Article  CAS  Google Scholar 

  • Bashir H, Gangwar R, Mishra S (2015) Differential production of lignocellulolytic enzymes by a white rot fungus Termitomyces sp. OE147 on cellulose and lactose. Biochim Biophys Acta 1854:1290–1299

    Article  CAS  Google Scholar 

  • Baun DL, Christensen TH (2004) Speciation of heavy metals in landfill leachate: a review. Waste Manage Res 22(1):3–23

    Article  CAS  Google Scholar 

  • Bortolotto T, Bertoldo JB, Da-Silveira FZ, Defaveri TM, Silvano J, Picha CT (2009) Evaluation of the toxic and genotoxic potential of landfill leachates using bioassays. Environ Toxicol Pharmacol 28(2):288–293

    Article  CAS  Google Scholar 

  • Chen Y, Liu Ch, Nie J, Wu S, Wang D (2014) Removal of COD and decolorizing from landfill leachate by Fenton’s reagent advanced oxidation. Clean Technol Environ Policy 16:189–193

    Article  CAS  Google Scholar 

  • Colussi F, Garcia W, Rosseto FR, Soares de Mello BL, de Oliveira Neto M, Polikarpov I (2012) Effect of pH and temperature on the global compactness, structure, and activity of cellobiohydrolase Cel7A from Trichoderma harzianum. Eur Biophys J 41:89–98

    Article  CAS  Google Scholar 

  • Deguchi Y, Toyoizumi T, Masuda S, Yasuhara A, Mohri S, Yamada M, Inoue Y, Kinae N (2007) Evaluation of mutagenic activities of leachates in landfill sites by micronucleus test and comet assay using goldfish. Mutat Res 627:178–185

    Article  CAS  Google Scholar 

  • Di-Iaconi C, Ramadori R, Antonio L (2006) Combined biological and chemical degradation for treating a mature municipal landfill leachate. Biochem Eng J 31:118–124

    Article  CAS  Google Scholar 

  • Espinosa-Ortiz EJ, Rene ER, Pakshirajan K, van Hullebusch ED, Lens PNL (2016) Fungal pelleted reactors in wastewater treatment: applications and perspectives. Chem Eng J 283:553–571

    Article  CAS  Google Scholar 

  • Freitas AC, Ferreira F, Costa AM, Pereira R, Antunes SC, Gonçalves F, Rocha-Santos TAP, Diniz MS, Castro L, Peres I, Duarte AC (2009) Biological treatment of the effluent from a bleached kraft pulp mill using basidiomycete and zygomycete fungi. Sci Total Environ 407:3282–3289

    Article  CAS  Google Scholar 

  • Gadd GM (1993) Interaction of fungi with toxic metals. New Phytol 124:25–60

    Article  CAS  Google Scholar 

  • Ghosh P, Swati, Thakur IS (2014a) Enhanced removal of COD and color from landfill leachate in a sequential bioreactor. Bioresour Technol 170:10–19

    Article  CAS  Google Scholar 

  • Ghosh P, Das MT, Thakur IS (2014b) Mammalian cell line-based bioassays for toxicological evaluation of landfill leachate treated by Pseudomonas sp. ISTDF1. Environ Sci Pollut Res 21:8084–8094

    Article  CAS  Google Scholar 

  • Gianfreda L, Rao MA, Scelza R, de la Luz Mora M (2016) Chapter-6: role of enzymes in environment cleanup/remediation. Agro-Industrial Wastes as Feedstock for Enzyme Production. ISBN 978-0-12-802392-1. doi: 10.1016/B978-0-12-802392-1.00006-X, pp 133–155

  • Gunderson CA, Kostuk JM, Gibbs MH, Napolitano GE, Wicker LF, Richmond JE, Stewart AJ (1997) Multispecies toxicity assessment of compost produced in bioremediation of an explosives-contaminated sediment. Environ Toxicol Chem 16(12):2529–2537

    Article  CAS  Google Scholar 

  • Guo JS, Abbas AA, Chen YP, Liu ZP, Fang F, Chen P (2010) Treatment of landfill leachate using a combined stripping, Fenton, SBR, and coagulation process. J Hazard Mater 178:699–705

    Article  CAS  Google Scholar 

  • Gupta A, Rajamani P (2015) Toxicity assessment of municipal solid waste landfill leachate collected in different seasons from Okhala landfill site of Delhi. J Biomedical Sci Eng 8:357–369

    Article  CAS  Google Scholar 

  • Hu L, Zeng G, Chen G, Dong H, Liu Y, Wan J, Chen A, Guo Z, Yan M, Wu H, Yu Z (2016) Treatment of landfill leachate using immobilized Phanerochaete chrysosporium loaded with nitrogen-doped TiO2 nanoparticles. J Hazard Mater 301:106–118

    Article  CAS  Google Scholar 

  • Kadri T, Rouissi T, Brar SK, Cledon M, Sarma S, Verma M (2016) Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by fungal enzymes: a review. J Environ Sci. doi:10.1016/j.jes.2016.08.023

    Google Scholar 

  • Kalcikova G, Vavrova M, Zagorc-Koncan J, Gotvajn AZ (2011) Evaluation of the hazardous impact of landfill leachates by toxicity and biodegradability tests. Environ Technol 32(12):1345–1353

    Article  CAS  Google Scholar 

  • Kalcikova G, Babic J, Pavko A, Gotvajn AZ (2014) Fungal and enzymatic treatment of mature municipal landfill leachate. Waste Manage 34:798–803

    Article  CAS  Google Scholar 

  • Kalcikova G, Zupancic M, Levei EA, Miclean M, Englande AJ, Gotvajn AZ (2015) Application of multiple toxicity tests in monitoring of landfill leachate treatment efficiency. Environ Monit Assess 187(8):489–503

    Article  Google Scholar 

  • Kalcikova G, Pirc ET, Gotvajn AZ (2016) Aerobic and anaerobic biodegradation potential of leachate from old active landfill. Desalination Water Treat 57:8619–8625

    Article  CAS  Google Scholar 

  • Karbassi A, Pazoki M (2015) Optimization of coagulation/flocculation for treatment of wastewater. J Environ Treat Tech 3(2):170–174

    Google Scholar 

  • Kaushik G, Gopal M, Thakur IS (2010) Evaluation of performance and community dynamics of microorganisms during treatment of distillery spent wash in a three stage bioreactor. Bioresour Technol 101:4296–4305

    Article  CAS  Google Scholar 

  • Knie JLW, Lopes EWB (2004) Testes ecotoxicológicos: métodos, técnicas e aplicações. FATMA, Florianópolis, p 289

    Google Scholar 

  • Kummerova M, Kmentova E (2004) Photo-induced toxicity of fluoranthene on germination and early development of plant seedling. Chemosphere 56:387–393

    Article  CAS  Google Scholar 

  • Kurniawan TA, Lo WH, Chan GYS (2006) Physico-chemical treatments for removal of recalcitrant contaminants from landfill leachate. J Hazard Mater 129:80–100

    Article  CAS  Google Scholar 

  • Li G, Yun Y, Li H, Sang N (2008) Effect of landfill leachate on cell cycle, micronucleus, and sister chromatid exchange in Triticum aestivum. J Hazard Mater 155:10–16

    Article  CAS  Google Scholar 

  • Mishra M, Thakur IS (2010) Isolation and characterization of alkalotolerant bacteria and optimization of process parameters for decolorization and detoxification of pulp and paper mill effluent by Taguchi approach. Biodegradation 21:967–978

    Article  CAS  Google Scholar 

  • Monavari SM, Omrani GA, Karbassi A, Raof FF (2012) The effects of socioeconomic parameters on household solid-waste generation and composition in developing countries (a case study: Ahvaz, Iran). Environ Monit Assess 184:1841–1846

    Article  Google Scholar 

  • Oral R, Meriç S, De-Nicola E, Petruzzelli D, Rocca CD, Pagano G (2007) Multi-species toxicity evaluation of a chromium-based leather tannery wastewater. Desalination 211:48–57

    Article  CAS  Google Scholar 

  • Pandey AK, Jamaluddin, Awasthi AK, Pandey A (2013) Biosorption potential of indigenous fungal strains for municipal solid waste leachate management in Jabalpur city. JECET 2(2):385–393

    Google Scholar 

  • Pant D, Adholeya A (2010) Development of a novel fungal consortium for the treatment of molasses distillery wastewater. Environmentalist 30:178–182

    Article  Google Scholar 

  • Pazoki M, Abdoli MA, Karbassi A, Mehrdadi N, Yaghmaeian K (2014) Attenuation of municipal landfill leachate through land treatment. J Environ Health Sci Eng 12:12

    Article  Google Scholar 

  • Rizzo L (2011) Bioassays as a tool for evaluating advanced oxidation processes in water and wastewater treatment. Water Res 45(15):4311–4340

    Article  CAS  Google Scholar 

  • Rodrigues FS, Bila DM, Campos JC, Sant’Anna GL Jr, Dezotti M (2009) Sequential treatment of an old-landfill leachate. Int J Environ Waste Manage 4:445–456

    Article  CAS  Google Scholar 

  • Saetang J, Babel S (2010) Effect of glucose on enzyme activity and color removal by Trametes versicolor for high strength landfill leachate. Water Sci Technol 62(11):2519–2526

    Article  CAS  Google Scholar 

  • Saetang J, Babel S (2012) Biodegradation of organics in landfill leachate by immobilized white rot fungi, Trametes versicolor BCC 8725. Environ Technol 33(22):2575–2584

    Article  CAS  Google Scholar 

  • Sang N, Han M, Li G, Huang M (2010) Landfill leachate affects metabolic responses of Zea mays L. seedlings. Waste Manage 30:856–862

    Article  CAS  Google Scholar 

  • Shahriari T, Karbassi AR, Tajziehchi S, Pouyandeh F, Nouri J (2016) Association of metals with various soil phases in kahrizak landfill. Transylv Rev 24:9

    Google Scholar 

  • Singh D, Chen S (2008) The white-rot fungus Phanerochaete chrysosporium: conditions for the production of lignin-degrading enzymes. Appl Microbiol Biotechnol 81:399–417

    Article  CAS  Google Scholar 

  • Singh P, Thakur IS (2006) Colour removal of anaerobically treated pulp and paper mill effluent by microorganisms in two steps bioreactor. Bioresour Technol 97:218–223

    Article  CAS  Google Scholar 

  • Tang WW, Zeng GM, Gong JL, Liang J, Xu P, Zhang C, Huang BB (2014) Impact of humic/fulvic acid on the removal of heavy metals from aqueous solutions using nanomaterials: a review. Sci Total Environ 468–469:1014–1027

    Article  Google Scholar 

  • Tigini V, Prigione V, Di-Toro S, Fava F, Varese GC (2009) Isolation and characterisation of polychlorinated biphenyl (PCB) degrading fungi from a historically contaminated soil. Microb Cell Fact 8:5

    Article  Google Scholar 

  • Tigini V, Spina F, Romagnolo A, Prigione V, Varese GC (2013) Effective biological treatment of landfill leachates by means of selected white rot fungi. Chem Eng Trans 32:265–270

    Google Scholar 

  • Torres E, Bustos-Jaimes I, Le-Borgne S (2003) Potential use of oxidative enzymes for the detoxification of organic pollutants. Appl Catal B Environ 46:1–15

    Article  CAS  Google Scholar 

  • U.S. Environmental Protection Agency–USEPA (1996) Proposed guidelines for ecological risk assessment. Risk Assessment Forum, Washington. (EPA 630-R95-002B)

  • Wan-Razarinah WAR, Zalina MN, Abdullah N (2014) Treatment of landfill leachate by immobilized Ganoderma australe and crude enzyme. Sci Asia 40(5):335–339

    Article  Google Scholar 

  • Wesenberg D, Kyriakides I, Agathos SN (2003) White-rot fungi and their enzymes for the treatment of industrial dye effluents. Biotechnol Adv 22:161–187

    Article  CAS  Google Scholar 

  • Woldeyohans AM, Worku T, Kloos H, Mulat W (2014) Treatment of leachate by recirculating through dumped solid waste in a sanitary landfill in Addis Ababa, Ethiopia. Ecol Eng 73:254–259

    Article  Google Scholar 

  • Xu P, Zeng GM, Huang DL, Feng CL, Hu S, Zhao MH, Lai C, Wei Z, Huang C, Xie GX, Liu ZF (2012) Use of iron oxide nanomaterials in wastewater treatment: a review. Sci Total Environ 424:1–10

    Article  CAS  Google Scholar 

  • Yosr S, Moncef C, Sami S, Jalel B (2016) Coagulation–flocculation process for landfill leachate pretreatment and optimization with response surface methodology. Desalination Water Treat 57(31):14488–14495

    Article  Google Scholar 

  • Zafar S, Aqil F, Ahmad I (2007) Metal tolerance and biosorption potential of filamentous fungi isolated from metal contaminated agricultural soil. Bioresour Technol 98:2557–2561

    Article  CAS  Google Scholar 

  • Zouboulis AI, Loukidou MX, Christodoulou K (2001) Enzymatic treatment of sanitary landfill leachate. Chemosphere 44:1103–1108

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors are thankful Head, Department of Biological Science, R.D. University, Jabalpur (M.P.), India, for providing laboratory facilities and also thankful to Municipal Corporation Jabalpur (M.P.) India, for their support.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to A. K. Awasthi.

Additional information

Editorial responsibility: H.K. Pant.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Awasthi, A.K., Pandey, A.K. & Khan, J. Potential of fungus Trichoderma harzianum for toxicity reduction in municipal solid waste leachate. Int. J. Environ. Sci. Technol. 14, 2015–2022 (2017). https://doi.org/10.1007/s13762-017-1271-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13762-017-1271-9

Keywords

Navigation