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Assessment of residue melamine in dairy products exhibited in Zanjan market, Iran by high-performance liquid chromatography method

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Abstract

An increasing number of infants are being made sick by dairy products contaminated with melamine. As a result, many countries have adopted additional requirements for milk-based food products. The scope of the present study was to assess amounts of melamine in some dairy products from Zanjan market, Iran. Amounts of melamine in samples of milk, yoghurt, infant formula, coffee mate and cheese were determined in five different brands by the high-performance liquid chromatography with ultraviolet detector system. In total, 225 samples were determined (five products × five brands × three samples × three repeated measurements). Amounts of melamine in samples (in μg/g) were determined in the range of 0.20–0.26 for milk, 0.57–0.99 for yoghurt, 0.35–3.40 for infant formula, 0.09–1.23 for coffee mate and 0.30–2.50 for cheese, and the mean values of melamine in products were evaluated as 0.24, 0.76, 1.38, 0.56 and 1.16, respectively. The minimum and maximum values for amounts of melamine were recorded in milk and infant formula, respectively. Comparison of test results with the standards set by the European Union and the US Food and Drug Administration for maximum residue-level values for dairy products contaminated with melamine showed that levels determined by tests had higher health risks than the regulations allowed. Iran has not yet adopted regulations to control levels of melamine in food products. The results show that maximum residue-level values need to be determined for melamine in Iranian dairy products, and foods should be monitored daily for levels of melamine contamination.

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References

  • Afoakwa EO (2008) Melamine contamination of infant formula in China: the causes, food safety issues and public health implications. Afr J Food Agric Nutr Dev (AJFAND) 8:1–9. http://works.bepress.com/emmanueloheneafoakwa/93

  • Chang H, Shi X, Shen W, Wang W, Yue Z (2012) Characterization of melamine-associated urinary stones in children with consumption of melamine-contaminated infant formula. Clin Chim Acta 14:985–991. doi:10.1016/j.cca.2012.02.025

    Article  Google Scholar 

  • Cheng TT (1994) Characterization of (Methoxymethyl)melamine Resins by liquid chromatography/mass spectrometry. Anal Chem 66:3267–3273. doi:10.1021/ac00091a042

    Article  Google Scholar 

  • Cheng Y, Dong Y, Wu J, Yang X, Bai H, Zheng H, Ren D, Zou Y, Li M (2010) Screening melamine adulterant in milk powder with laser Raman spectrometry. J Food Compos Anal 23:199–202. doi:10.1016/j.jfca.2009.08.006

    Article  CAS  Google Scholar 

  • Feng W, Lv C, Yang L, Cheng J, Yan C (2012) Determination of melamine concentrations in dairy samples. LWT Food Sci Technol 47:147–153. doi:10.1016/j.lwt.2011.12.021

    Article  CAS  Google Scholar 

  • Filazi A, Sireli UT, Ekici H, Can HY, Karagoz A (2012) Determination of melamine in milk and dairy products by high performance liquid chromatography. J Dairy Sci 95:602–608. doi:10.3168/jds.2011-4926

    Article  CAS  Google Scholar 

  • Ge J, Zhao LW, Liu C, Jiang S, Lee PW, Liu F (2011) Rapid determination of melamine in soil and strawberry by liquid chromatography–tandem mass spectrometry. Food Control 22:1629–1633. doi:10.1016/j.foodcont.2011.03.020

    Article  CAS  Google Scholar 

  • Gossner CM, Schlundt J, Embarek PB, Hird S, Wong DL-F, Beltran JJ et al (2009) The melamine incident: implications for international food and feed safety. Environ Health Perspect 117:1803–1808. doi:10.1289/ehp.0900949

    Article  CAS  Google Scholar 

  • Hau AKC, Kwan TH, Li PK (2009) Melamine toxicity and the kidney. J Am Soc Nephol 20:245–250. doi:10.1681/ASN.2008101065

    Article  CAS  Google Scholar 

  • Haughey SA, Graham SF, Cancouët E, Elliott CT (2013) The application of near-infrared reflectance spectroscopy (NIRS) to detect melamine adulteration of soya bean meal. Food Chem, 1557–1561. doi:10.1016/j.foodchem.2012.01.068

  • Heintzelman L (2011) Melamine contamination. Encycl Bioterr Def, 1–4. doi:10.1002/0471686786.ebd0171

  • Hong M, Sai F, Yong-Ning W, Ping-Ping Z, Hui-Jing C, Yug-Feng Z (2009) Simultaneous determination of melamine, ammelide, ammeline, and cyanuric acid in milk and milk products by gas chromatography–tandem mass spectrometry. Biomed Environ Sci 2:87–94. doi:10.1016/S0895-3988(09)60027-1

    Article  Google Scholar 

  • Langman CB (2009) Melamine, powdered milk and nephrolithiasis in Chinese infants. N Engl J Med 360:1139–1143

    Article  CAS  Google Scholar 

  • Li J, Qi H-Y, Shi Y-P (2009) Determination of melamine residues in milk products by zirconia hollow fibersorptive microextraction and gas chromatography–mass spectrometry. J Chromatogr A 29:5467–5471. doi:10.1016/j.chroma.2009.05.047

    Article  Google Scholar 

  • Li L, Li B, Cheng D, Mao L (2010) Visual detection of melamine in raw milk using gold nanoparticles as colorimetric probe. Food Chem 122:895–900. doi:10.1016/j.foodchem.2010.03.032

    Article  CAS  Google Scholar 

  • Liu Y, Todd EE, Zhang Q, Shi J-R, Liu X-J (2012) Recent developments in the detection of melamine. J Zhejiang Univ Sci B (Biomed Biotechnol) 13:525–532. doi:10.1631/jzus.B1100389

    Article  CAS  Google Scholar 

  • Qin Y, Lv X, Li J, Qi G, Diao Q, Liu G, Xue M, Wang J, Tong J (2010) Assessment of melamine contamination in crop, soil and water in China and risks of melamine accumulation in animal tissues and products. Environ Int 36:446–452. doi:10.1016/j.envint.2010.03.006

    Article  CAS  Google Scholar 

  • Qin J, Chao K, Kim MS (2013) Simultaneous detection of multiple adulterants in dry milk using macro-scale Raman chemical imaging. Food Chem 138:998–1007. doi:10.1016/j.foodchem.2012.10.115

    Article  CAS  Google Scholar 

  • Reimschuessel R, Hattan D, Gu Y (2009) Background paper on toxicology of melamine and its analoges. World Health Organization, Geneva

    Google Scholar 

  • Salman M, Hamed E-SS, Al-Amoudi MS, Salman L, Alghamdi MT, Bazaid SA (2012) Identification and determination of melamine in milk by high performance liquid chromatography–UV-Detector. Der Pharma Chemica 4:737–748

    CAS  Google Scholar 

  • Tian Y, Chen L, Gao L, Wu M, Dick WA (2012) Comparison of three methods for detection of melamine in compost and soil. Sci Total Environ 417:255–262. doi:10.1016/j.scitotenv.2011.12.014

    Article  Google Scholar 

  • Tyan YC, Yang MH, Jong SB, Wang CK, Shiea J (2009) Melamine contamination. Anal Bioanal Chem 395:729–735. doi:10.1007/s00216-009-3009-0

    Article  CAS  Google Scholar 

  • Vachirapatama N, Maitresorsun S (2013) Simultaneous determination of melamine, ammelide, ammeline and cyanuric acid in milk products by Micellar Electrokinetic chromatography. J Food Drug Anal 21:66–72. doi:10.6227/jfda.2013210108

    CAS  Google Scholar 

  • Venkatasami G, Sowa JR Jr (2010) A rapid, acetonitrile-free, HPLC method for determination of melamine in infant formula. Anal Chim Acta 665:227–230. doi:10.1016/j.aca.2010.03.037

    Article  CAS  Google Scholar 

  • Veritas B (2008) Melamine in food products: worldwide requirements. Bureau Veritas Consumer Products Services

  • Wang IJ, Chen P-C, Hwang K-C (2009) Melamine and nephrolithiasis in children in Taiwan. N Engl J Med 360:1157–1158. doi:10.1056/NEJMc0810070

    Article  CAS  Google Scholar 

  • Wang J, Jiang L, Chu O, Ye J (2010) Residue analysis of melamine in milk products by micellar electrokinetic capillary chromatography with amperometric detection. Food Chem 121:215–219. doi:10.1016/j.foodchem.2009.11.074

    Article  CAS  Google Scholar 

  • WHO (2008) Melamine and cyanuric acid: toxicity, preliminary risk assessment and guidance on levels in food. World Health Organization

  • WHO (2009a) Background paper on methods for the analysis of melamine and related compounds in foods and animal feeds. World Health Organization, Geneva

    Google Scholar 

  • WHO (2009b) Background paper on dietary exposure assessment. World Health Organization, Geneva

    Google Scholar 

  • Xu X, Ren Y, Zhu Y, Cai Z, Han J, Huang B, Yan Z (2009) Direct determination of melamine in dairy products by gas chromatography/mass spectrometry with coupled column separation. Anal Chim Acta 650:39–43. doi:10.1016/j.aca.2009.04.026

    Article  CAS  Google Scholar 

  • Yin RH, Wang XZ, Bai WL, Wu CD, Yin RL, Li C, Liu J, Liu BS, He JB (2012) The reproductive toxicity of melamine in the absence and presence of cyanuric acid in male mice. Res Vet Sci 94(3):618–627. doi:10.1016/j.rvsc.2012.11.010

    Article  Google Scholar 

  • Zakeel M (2008) Melamine: do you know what it is? http://www.sundaytimes.lk/081102/Plus/sundaytimesplus_14.html

  • Zamani A, Yaftian M, Parizanganeh A (2012) Multivariate statistical assessment of heavy metal pollution sources of groundwater around a lead and zinc plant. Iranian J Environ Health Sci Eng 9:1–10. doi:10.1186/1735-2746-9-29

    Article  Google Scholar 

  • Zeng H, Yang R, Wang QW, Li JJ, Qu LB (2011) Determination of melamine by flow injection analysis based on chemiluminescence system. Food Chem 127:842–846. doi:10.1016/j.foodchem.2011.01.021

    Article  CAS  Google Scholar 

  • Zhang M, Cao X, Li H, Guan F, Cuo J, Shen F, Luo Y, Sun C, Zhang L (2012) Sensitive fluorescent detection of melamine in raw milk based on the inner filter effect of Au nanoparticles on the fluorescence of CdTe quantum dots. Food Chem 135:1894–1900. doi:10.1016/j.foodchem.2012.06.070

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Sincere gratitude to the University of Zanjan (Zanjan- Iran) for financial support of this study. The Grant Number is 8445. The authors gratefully acknowledge Masoud Hajisoleimani and Hossein Soltani, Phase Equilibria Research Laboratory, for their contribution to this work.

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Authors and Affiliations

  1. Environmental Science Research Laboratory, Department of Environmental Science, Faculty of Science, University of Zanjan, 45371-38791, Zanjan, Iran

    N. Poorjafari, A. Zamani & A. Parizanganeh

  2. Department of Food and Drug Control, School of Paramedical and Health, Zanjan University of Medical Science, 45139-56184, Zanjan, Iran

    M. Mohseni

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  1. N. Poorjafari
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  2. A. Zamani
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  3. M. Mohseni
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  4. A. Parizanganeh
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Correspondence to A. Zamani.

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Poorjafari, N., Zamani, A., Mohseni, M. et al. Assessment of residue melamine in dairy products exhibited in Zanjan market, Iran by high-performance liquid chromatography method. Int. J. Environ. Sci. Technol. 12, 1003–1010 (2015). https://doi.org/10.1007/s13762-014-0707-8

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  • DOI: https://doi.org/10.1007/s13762-014-0707-8

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