Abstract
In this study, monthly and daily samplings were carried out at Klang, an eutrophic estuary, and at Port Dickson, an oligotrophic coastal water system. Escherichia coli concentration was measured via culture method, and the phylogenetic structure of E. coli population was via Clermont typing. Average E. coli concentration at Klang was higher than Port Dickson (t = 2.97, df = 10, p < 0.05), and daily sampling did not show any apparent temporal variation at both sites. At Klang, salinity was inversely correlated with coliform (R 2 = 0.216, df = 25, p < 0.05), suggesting that river flow was a mode of transport for coliform. Although E. coli concentration was higher at the eutrophic site, E. coli population structure at both Klang and Port Dickson were similar and showed neither long-term nor short-term variations. This study showed the predominance of commensal groups A and B1 in tropical coastal waters of Peninsular Malaysia.
Similar content being viewed by others
References
Bukh AS, Schønheyder HC, Emmersen JMG, Søgaard M, Bastholm S, Roslev P (2009) Escherichia coli phylogenetic groups are associated with site of infection and level of antibiotic resistance in community-acquired bacteraemia: a 10 year population-based study in Denmark. J Antimicrob Chemother 64:163–168
Cho KH, Cha SM, Kang JH, Lee SW, Park JW, Kim JH (2010) Meteorological effects on the levels of fecal indicator bacteria in an urban stream: a modelling approach. Water Res 44(7):2189–2202
Chua TE, Gorre IRL, Ross SA, Bernad SR, Gervacio B, Ebarvia MC (2000) The Malacca straits. Mar Pollut Bull 41:160–178
Clermont O, Bonacorsi S, Bingen E (2000) Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol 66:4555–4558
Department of Environment (2009) 2009 Annual Report. Department of Environment, Ministry of Natural Resources and Environment, Kuala Lumpur, pp 84–86
Duriez P, Clermont O, Bonacorsi S, Bingen E, Chaventré A, Elion J, Picard B, Denamur E (2001) Commensal Escherichia coli isolates are phylogenetically distributed among geographically distinct human populations. Microbiology 147:1671–1676
Escobar-Páramo P, Grenet K, Le Menac’h A, Rode L, Salgado E, Amorin C, Gouriou S, Picard B, Rahimy MC, Andremont A, Denamur E, Ruimy R (2004) Large-scale population structure of human commensal Escherichia coli isolates. Appl Environ Microbiol 70:5698–5700
Ferguson C, Husman AM, Altavilla N, Deere D, Ashbolt N (2003) Fate and transport of surface water pathogens in watersheds. Crit Rev Environ Sci Technol 33:299–361
Gabutti G, De Donno A, Erroi R, Liaci D, Bagordo F, Montagna MT (2004) Relationship between indicators of faecal pollution and presence of pathogenic microorganism in coastal seawaters. J Coast Res 20:846–852
Garrity GM, Brenner DJ, Krieg NR, Staley JR (Eds.) (2005) Bergey’s manual of systematic bacteriology, vol 2: the Proteobacteria, part b: the Gammaproteobacteria (2nd Ed.). Springer, New York, p 607–624
Geldreich EE (1996) Pathogenic agents in freshwater resources. Hydrol Process 10:315–333
Gordon DM, Clermont O, Tolley H, Denamur E (2008) Assigning Escherichia coli strains to phylogenetic groups: multi-locus sequence typing versus the PCR triplex method. Environ Microbiol 10:2484–2496
Grasshoff K, Kremling K, Ehrhardt M (1999) Methods of seawater analysis, 3rd edn, Wiley-VCH, Weinhein, pp 75–89
Herzer PJ, Inouye S, Inouye M, Whittam TS (1990) Phylogenetic distribution of branched RNA-linked multicopy single-stranded DNA among natural isolates of Escherichia coli. J Bacteriol 172:6175–6181
Higgins J, Hohn C, Hornor S, Frana M, Denver M, Joerger R (2007) Genotyping of Escherichia coli from environmental and animal samples. J Microbiol Meth 70:227–235
Kenzaka T, Yamaguchi N, Prapagdee B, Mikami E, Nasu M (2001) Bacterial community composition and activity in urban rivers in Thailand and Malaysia. J Health Sci 47:353–361
Lai FS (1983) Biochemical oxygen demand concentration of two river basins in Selangor. Pertanika 6(3):32–43
Law AT (1980) Sewage pollution in Kelang River and its estuary. Pertanika 3:13–19
Lee CW, Bong CW (2008) Bacterial abundance and production, and their relation to primary production in tropical coastal waters of Peninsular Malaysia. Mar Freshw Res 59:10–21
Lee CW, Bong CW, Hii YS (2009) Temporal variation of bacterial respiration and growth efficiency in tropical coastal waters. Appl Environ Microbiol 75(24):7594–7601
Lee CW, Ng AYF, Bong CW, Narayanan K, Sim EUH, Ng CC (2011) Investigating the decay rates of Escherichia coli relative to Vibrio parahaemolyticus and Salmonella Typhi in tropical coastal waters. Water Res 45:1561–1570
Li B, Sun JY, Han LZ, Huang XH, Fu Q, Ni YX (2010) Phylogenetic groups and pathogenicity island markers in fecal Escherichia coli isolates from asymptomatic humans in China. Appl Environ Microbiol 76(19):6698–6700
Orsi RH, Stoppe NC, Sato MIZ, Prado PI, Ottoboni LMM (2008) Phylogenetic group distribution among Escherichia coli isolated from rivers in São Paulo State, Brazil. World J Microbiol Biotechnol 24:1573–1577
Picard B, Garcia JS, Gouriou S, Duriez P, Brahimi N, Bingen E, Elion J, Denamur E (1999) The link between phylogeny and virulence in Escherichia coli extraintestinal infection. Infect Immun 67:546–553
Poté J, Goldscheider N, Haller L, Zopfi J, Khajehnouri F, Wildi W (2009) Origin and spatial-temporal distribution of faecal bacteria in a bay of Lake Geneva, v. Environ Monit Assess 154:337–348
Ratajczak M, Laroche E, Thierry B, Clermont O, Pawlak B, Denamur E, Petit F (2010) Influence of hydrological conditions on the Escherichia coli population structure in the water of a creek on a rural watershed. BMC Microbiol 10:222
Tenaillon O, Skurnik D, Picard B, Denamur E (2010) The population genetics of commensal Escherichia coli. Nature Rev Microbiol 8:207–217
Unno T, Han D, Jang J, Lee SN, Go G, Choi HY, Kim JH, Sadowsky MJ, Hur HG (2009) Absence of Escherichia coli phylogenetic group B2 strains in humans and domesticated animals from Jeonam Province, Korea. Appl Environ Microbiol 75:5659–5666
Walk ST, Alm EW, Calhoun LM, Mladonicky JM, Whittam TS (2007) Genetic diversity and population structure of Escherichia coli isolated from freshwater beaches. Environ Microbiol 9:2274–2288
Wilkinson J, Kay D, Wyer M, Jenkins A (2006) Processes driving the episodic flux of faecal indicator organisms in streams impacting on recreational and shellfish harvesting waters. Water Res 40:153–161
Acknowledgments
This work was supported by University of Malaya (PS 141-2008A, UM.C/625/1/HIR/050).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Tan, K.W., Lee, C.W. Long-term and short-term variations of Escherichia coli population structure in tropical coastal waters. Int. J. Environ. Sci. Technol. 11, 711–718 (2014). https://doi.org/10.1007/s13762-013-0367-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13762-013-0367-0