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Effects of extremely low frequency electromagnetic field (50 Hz) on pentylenetetrazol-induced seizures in mice

Abstract

The electromagnetic fields (EMF) have various behavioral and biological effects on human body. There are growing concerns about the consequences of exposure to EMF. However, some studies have shown beneficial effects of these waves on human. In this paper, we study the effect of acute, sub acute and long-term exposure to 50 Hz, 0.1 mT magnetic fields (MF) on the seizure induction threshold in mice. 64 mice are used and divided into four groups. Eight mice in any group were selected to be exposed to MF for specific duration and the others were used as a control group. The duration of the applied exposures was as follows: (1) 1 day (acute), (2) 3 days (sub acute), (3) 2 weeks (sub acute), (4) 1 month (long term). The mice were exposed 2 h for a day. After exposure, the pentylentetrazol (PTZ) is injected to the mice to induce seizure and the needed dose for the seizure induction threshold is measured. In the acute exposure, the threshold to induce seizure in the exposed and sham-exposed groups was 44.25 and 46.5 mg, respectively, while the difference was not significant (p value = 0.5). In the sub acute exposure (3 days), the mean amount of drug to induce seizure was 47.38 mg in the exposed and 43.88 mg in the sham-exposed groups, however, the difference was not significant (p value = 0.3). The results were 52.38 and 46.75 mg after 2 weeks of exposure which were not significantly different either (p value = 0.2). After 1 month of exposure to MF, the threshold for the induction of seizure was significantly increased (p value < 0.05). The mean dosage to induce seizure in the exposed and control group was 54.3 and 45.75 mg, respectively. However, considering the p value, the difference in the seizure induction threshold between the exposed and sham-exposed groups after acute and sub acute exposure was not significant, analyzing the effects of acute, sub acute and long-term exposures totally indicates that increasing the exposure time increases the seizure induction threshold.

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References

  1. Zahiroddin AR, Kandjani ARS, Hezaveh NM (2006) Mental health status of employees in substations of electromagnetic fields at extremely low frequency in Tehran. Iran J Environ Health Sci Eng 3(3):217–221

    Google Scholar 

  2. Sienkiewicz Z, Jones N, Bottomley A (2005) Neurobehavioural effects of electromagnetic fields. Bioelectromagnetics. 26(Suppl 7):S116–S126

    Article  Google Scholar 

  3. Bell GB, Marino AA, Chesson AL (1992) Alterations in brain electrical activity caused by magnetic fields: detecting the detection process. Electroencephalogr Clin Neurophysiol 83(6):389–397

    PubMed  Article  CAS  Google Scholar 

  4. Bell GB, Marino AA, Chesson AL (1994) Frequency-specific blocking in the human brain caused by electromagnetic fields. NeuroReport 5(4):510–512

    PubMed  Article  CAS  Google Scholar 

  5. Sobel E, Davanipour Z (1996) Electromagnetic field exposure may cause increased production of amyloid beta and eventually lead to Alzheimer’s disease. Neurology 47(6):1594–1600

    PubMed  Article  CAS  Google Scholar 

  6. Lyskov EB, Juutilainen J, Jousmaki V, Partanen J, Medvedev S, Hanninen O (1993) Effects of 45-Hz magnetic fields on the functional state of the human brain. Bioelectromagnetics 14(2):87–95

    PubMed  Article  CAS  Google Scholar 

  7. Potschka H, Thun-Battersby S, Loscher W (1998) Effect of low-intensity 50-Hz magnetic fields on kindling acquisition and fully kindled seizures in rats. Brain Res 809(2):269–276

    PubMed  Article  CAS  Google Scholar 

  8. Michon AL, Persinger MA (1997) Experimental simulation of the effects of increased geomagnetic activity upon nocturnal seizures in epileptic rats. Neurosci Lett 224(1):53–56

    PubMed  Article  CAS  Google Scholar 

  9. Persinger MA (1996) Enhancement of limbic seizures by nocturnal application of experimental magnetic fields that simulate the magnitude and morphology of increases in geomagnetic activity. Int J Neurosci 86(3–4):271–280

    PubMed  Article  CAS  Google Scholar 

  10. Keshavan MS, Gangadhar BN, Gautman RU, Ajit VB, Kapur RL (1981) Convulsive thresholds in humans and rats and magnetic field changes: observations during total solar eclipse. Neurosci Lett 22:205–208

    PubMed  Article  CAS  Google Scholar 

  11. Rajaram M, Mitra S (1981) Correlation between convulsive seizure and geomagnetic activity. Neurosci Lett 24:187–191

    PubMed  Article  CAS  Google Scholar 

  12. Cain DP, Corcoran ME (1985) Epileptiform effects of met-enkephalin, beta-endorphin and morphine: kindling of generalized seizures and potentiation of epileptiform effects by handling. Brain Res 338(2):327–336

    PubMed  Article  CAS  Google Scholar 

  13. Ossenkopp KP, Cain DP (1991) Inhibitory effects of powerline-frequency (60-Hz) magnetic fields on pentylenetetrazol-induced seizures and mortality in rats. Behav Brain Res 44(2):211–216

    PubMed  Article  CAS  Google Scholar 

  14. Sung JH, Jeong JH, Kim JS, Choi TS, Park JH, Kang HY et al (2003) The influences of extremely low frequency magnetic fields on drug-induced convulsion in mouse. Arch Pharm Res 26(6):487–492

    PubMed  Article  CAS  Google Scholar 

  15. Kavaliers M, Ossenkopp KP (1986) Stress-induced opioid analgesia and activity in mice: inhibitory influences of exposure to magnetic fields. Psychopharmacology 89(4):440–443

    PubMed  Article  CAS  Google Scholar 

  16. Kavaliers M, Ossenkopp KP (1986) Magnetic fields differentially inhibit mu, delta, kappa and sigma opiate-induced analgesia in mice. Peptides 7(3):449–453

    PubMed  Article  CAS  Google Scholar 

  17. Ossenkopp KP, Kavaliers M (1987) Morphine-induced analgesia and exposure to low-intensity 60-Hz magnetic fields: inhibition of nocturnal analgesia in mice is a function of magnetic field intensity. Brain Res 418(2):356–360

    PubMed  Article  CAS  Google Scholar 

  18. Cain DP, Corcoran ME (1984) Intracerebral beta-endorphin, met-enkephalin and morphine: kindling of seizures and handling-induced potentiation of epileptiform effects. Life Sci 34(25):2535–2542

    PubMed  Article  CAS  Google Scholar 

  19. Balanezhad SZ, Parivar K, Baharara J, Kouchesfehani HM, Ashraf A (2010) The effect of extremely low frequency electromagnetic field on angiogenesis. Res J Environ Sci 4(3):300–304

    Article  Google Scholar 

  20. García AM, Sisternas A, Hoyos SP (2008) Occupational exposure to extremely low frequency electric and magnetic fields and Alzheimer disease: a meta-analysis. Int J Epidemiol. 37:329–340

    PubMed  Article  Google Scholar 

  21. Mairs RJ, Hughes K, Fitzsimmons S et al (2007) Microsatellite analysis for determination of the mutagenicity of extremely low-frequency electromagnetic fields and ionising radiation in vitro. Mutat Res 626:34–41

    PubMed  Article  CAS  Google Scholar 

  22. Jimenez-Garcia MN, Arellanes-Robledo J, Aparicio-Bautista DI et al (2010) Anti- proliferative effect of extremely low frequency electromagnetic field on preneoplastic lesions formation in the rat liver. BMC Cancer 10:159–171

    PubMed  Article  Google Scholar 

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The authors declare that they have no conflict of interest.

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Correspondence to Valiallah Saba.

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Fadakar, K., Saba, V. & Farzampour, S. Effects of extremely low frequency electromagnetic field (50 Hz) on pentylenetetrazol-induced seizures in mice. Acta Neurol Belg 113, 173–177 (2013). https://doi.org/10.1007/s13760-012-0133-y

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  • DOI: https://doi.org/10.1007/s13760-012-0133-y

Keywords

  • ELF
  • EMF
  • Exposure
  • Seizure
  • Pentylentetrazol (PTZ)