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Raw horse gram seeds possess more in vitro antihyperglycaemic activities and antioxidant properties than their sprouts


Plants have been used by humans as food and medicine since prehistoric times. However, in the course of modernisation and following the advent of various scientific terms such as functional foods, nutraceuticals, food additives, etc., the texture and matrix of traditional dietary practice has changed. These changes, in turn, have influenced nutritive as well as medicinal values of food preparations. Raw horse gram seeds have been used by economically disadvantaged people in tropical countries as a cheap source of protein. Recently, inclusion in the diet of sprouts of horse gram seeds has increased. In this research, we found that raw horse gram seed is a rich source of polyphenols, flavonoids and protein. It also possesses potent properties to scavenge free radicals and the ability to reduce starch-induced postprandial glycaemic excursions by virtue of potent intestinal α-glucosidase inhibitory activity. Furthermore, consumption of raw horse gram seeds can also reduce insulin resistance by inhibiting protein-tyrosine phosphatase 1β. Our analysis revealed that the effects of sprouting on antioxidant activities vary. The antioxidant activities were found to be concentrated more in the seed coat of the seeds. In addition, sprouting significantly decreased intestinal α-glucosidase and protein-tyrosine phosphatase 1β inhibitory activities. These observations suggest that consumption of food items prepared with unprocessed raw horse gram seeds may have more health benefits than their sprouts for hyperglycaemic individuals.

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  1. 1.

    Galli C (2012) Bioactive components in Mediterranean diets from historical and ethnobotanical considerations to nutraceutical applications. Nutrafoods 11:11–17

    Article  CAS  Google Scholar 

  2. 2.

    O’Keefe JH, Gheewala NM, O’Keefe JO (2008) Dietary strategies for improving post-prandial glucose, lipids, inflammation, and cardiovascular health. J Am Coll Cardiol 51:249–255

    Article  Google Scholar 

  3. 3.

    Tiwari AK, Praveen KM, Anand KD, Agawane SB, Madhusudana K, Zehra A (2012) Ayurvedic dietary formulations and postprandial glycemia in rats. Int Food Res J 19:765–773

    CAS  Google Scholar 

  4. 4.

    Giacco F, Brownlee M (2010) Oxidative stress and diabetic complications. Circ Res 107:1058–1070

    Article  CAS  Google Scholar 

  5. 5.

    Dubin HE, Corbitt HB (1923) On the nature of the action of vegetable extracts on the blood sugar of normal rabbits. Proc Soc Exp Biol Med 21:16–18

    Article  CAS  Google Scholar 

  6. 6.

    Tiwari AK, Reddy KS, Radhakrishnan J et al (2011) Influence of antioxidant rich fresh vegetable juices on starch induced postprandial hyperglycemia in rats. Food Funct 2:521–528

    Article  CAS  Google Scholar 

  7. 7.

    Tiwari AK, Anusha I, Sumangali M, Kumar DA, Madhusudana K, Agawane SB (2013) Preventive and therapeutic efficacies of Benincasa hispida and Sechium edule fruit’s juice on sweet-beverages induced impaired glucose tolerance and oxidative stress. Pharmacologia 4:197–207

    Article  Google Scholar 

  8. 8.

    Tiwari AK, Swapna M, Ayesha SB, Zehra A, Agawane SB, Madhusudana K (2011) Identification of proglycemic and antihyperglycemic activity in antioxidant rich fraction of some common food grains. Int Food Res J 18:915–923

    CAS  Google Scholar 

  9. 9.

    Prathapan A, Fahad K, Thomas BK, Philip RM, Raghu KG (2011) Effect of sprouting on antioxidant and inhibitory potential of two varieties of Bengal gram (Cicer arietinum L.) against key enzymes linked to type-2 diabetes. Int J Food Sci Nutr 62:234–238

    Article  CAS  Google Scholar 

  10. 10.

    Ghani A (1998) Medicinal plants of Bangladesh: chemical constituents and uses. Asiatic Society of Bangladesh, Dhaka

    Google Scholar 

  11. 11.

    Kawsar SMA, Huq E, Nahar N, Ozeki Y (2008) Identification and quantification of phenolic acids in Macrotyloma uniflorum by reversed phase HPLC. Am J Plant Physiol 3:165–172

    Article  CAS  Google Scholar 

  12. 12.

    Siddhuraju P, Manian S (2007) The antioxidant activity and free radical-scavenging capacity of dietary phenolic extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) seeds. Food Chem 105:950–958

    Article  CAS  Google Scholar 

  13. 13.

    Sreerama YN, Sashikala VB, Pratape VM (2012) Phenolic compounds in cowpea and horse gram flours in comparison to chickpea flour: evaluation of their antioxidant and enzyme inhibitory properties associated with hyperglycemia and hypertension. Food Chem 133:156–162

    Article  CAS  Google Scholar 

  14. 14.

    Laxmi HG, Sachin L, Bodhankar, Subhash L, Sushma SG (2011) Antidiabetic potential of á-amylase inhibitor from the seeds of Macrotyloma uniflorum in streptozotocin-nicotinamide-induced diabetic mice. Pharm Biol 49:182–189

    Article  Google Scholar 

  15. 15.

    Aruoma OI (1994) Deoxyribose assay for detecting hydroxyl radicals. Meth Enzymol 233:57–66

    Article  CAS  Google Scholar 

  16. 16.

    Patel RM, Patel NJ (2011) In vitro antioxidant of coumarin compounds by DPPH, superoxide and nitric oxide free radical scavenging methods. J Adv Pharm Educ Res 1:52–68

    Google Scholar 

  17. 17.

    Klomann SD, Mueller AS, Pallauf J, Krawinkel MB (2010) Antidiabetic effect of bitter gourd extracts in insulin-resistant db/db mice. Br J Nutr 104:1613–1620

    Article  CAS  Google Scholar 

  18. 18.

    Augustin J, Klein BP (1989) Nutrient composition of raw, cooked, canned and sprouted legumes. In: Matthews RH (ed.) Legumes: chemistry, technology and human nutrition. Marcel Dekker, New York, pp 187–217

    Google Scholar 

  19. 19.

    Goodman DBP, Davis WL (1993) Insulin accelerates the post germinative development of several fat-storing seeds. Biochem Biophys Res Commun 190:440–446

    Article  CAS  Google Scholar 

  20. 20.

    Pathak M, Martirosyan DM (2011) Immunodetection and quantification of insulin-like antigens in sprouts: development of an efficient functional food. Funct Foods Health Dis 1:492–507

    CAS  Google Scholar 

  21. 21.

    Prior RL, Wu X, Schaich K (2005) Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53:4290–4302

    Article  CAS  Google Scholar 

  22. 22.

    Kumar KG, Venkataraman LV (1976) Studies on the in vitro digestibility of starch in some legumes before and after germination. Nutr Rep Int 13:115–124

    CAS  Google Scholar 

  23. 23.

    Bravo L, Siddhuraju P, Saura-Calixto F (1998) Effect of various processing methods on the in vitro starch digestibility and resistant starch content of Indian pulses. J Agric Food Chem 46:4667–4674

    Article  CAS  Google Scholar 

  24. 24.

    McGill JB (2012) Pharmacotherapy in type 2 diabetes: a functional schema for drug classification. Curr Diabetes Rev 8:257–67

    Article  CAS  Google Scholar 

  25. 25.

    Popov D (2011) Novel protein tyrosine phosphatase 1B inhibitors: interaction requirements for improved intracellular efficacy in type 2 diabetes mellitus and obesity control. Biochem Biophys Res Commun 410:377–381

    Article  CAS  Google Scholar 

  26. 26.

    Mitrou PN, Kipnis V, Thiebaut AC et al (2007) Mediterranean dietary pattern and prediction of all-cause mortality in a US population: results from the NIH-AARP Diet and Health Study. Arch Intern Med 167:2461–2468

    Article  Google Scholar 

  27. 27.

    Valussi M, Scirè AS (2012) Quantitative ethnobotany and traditional functional foods A review. Nutrafoods 11:85–93

    Article  Google Scholar 

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Correspondence to Ashok Kumar Tiwari.

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Tiwari, A.K., Manasa, K., Kumar, D.A. et al. Raw horse gram seeds possess more in vitro antihyperglycaemic activities and antioxidant properties than their sprouts. Nutrafoods 12, 47–54 (2013).

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  • antioxidant activities
  • α-glucosidase inhibition
  • horse gram seed
  • Macrotyloma uniflorum
  • protein-tyrosine phosphatase 1 βinhibition