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Raw horse gram seeds possess more in vitro antihyperglycaemic activities and antioxidant properties than their sprouts

Abstract

Plants have been used by humans as food and medicine since prehistoric times. However, in the course of modernisation and following the advent of various scientific terms such as functional foods, nutraceuticals, food additives, etc., the texture and matrix of traditional dietary practice has changed. These changes, in turn, have influenced nutritive as well as medicinal values of food preparations. Raw horse gram seeds have been used by economically disadvantaged people in tropical countries as a cheap source of protein. Recently, inclusion in the diet of sprouts of horse gram seeds has increased. In this research, we found that raw horse gram seed is a rich source of polyphenols, flavonoids and protein. It also possesses potent properties to scavenge free radicals and the ability to reduce starch-induced postprandial glycaemic excursions by virtue of potent intestinal α-glucosidase inhibitory activity. Furthermore, consumption of raw horse gram seeds can also reduce insulin resistance by inhibiting protein-tyrosine phosphatase 1β. Our analysis revealed that the effects of sprouting on antioxidant activities vary. The antioxidant activities were found to be concentrated more in the seed coat of the seeds. In addition, sprouting significantly decreased intestinal α-glucosidase and protein-tyrosine phosphatase 1β inhibitory activities. These observations suggest that consumption of food items prepared with unprocessed raw horse gram seeds may have more health benefits than their sprouts for hyperglycaemic individuals.

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Correspondence to Ashok Kumar Tiwari.

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Tiwari, A.K., Manasa, K., Kumar, D.A. et al. Raw horse gram seeds possess more in vitro antihyperglycaemic activities and antioxidant properties than their sprouts. Nutrafoods 12, 47–54 (2013). https://doi.org/10.1007/s13749-013-0012-z

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Keywords

  • antioxidant activities
  • α-glucosidase inhibition
  • horse gram seed
  • Macrotyloma uniflorum
  • protein-tyrosine phosphatase 1 βinhibition