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Heterorhabditis amazonensis to Control Euschistus heros (Hemiptera: Pentatomidae) in Laboratory and Field Conditions

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Abstract

This study evaluated the potential of entomopathogenic nematodes (EPNs) to control Euschistus heros (Fabricius) under laboratory and field conditions. Eight isolates of the species Heterorhabditis amazonensis were evaluated on field-collected and laboratory-reared insect populations. The isolates that performed best in the virulence test were evaluated for in vivo production on Galleria mellonella Fabricius larvae. Then, these were evaluated in field conditions on E. heros from the laboratory and field populations. The isolates IBCB-n46, NEPET11, and IBCB-n40 caused the highest mortality in adult E. heros from laboratory rearing (100, 94, and 80%, respectively), and IBCB-n46 and NEPET11 were the best against the field population (71 and 47% mortality, respectively). In the assay to evaluate production, the isolate IBCB-n46 achieved the best result (3.4 × 105 IJs/g per larvae), followed by isolates UEPN05 (1.4 × 105 IJs/g per larvae) finally NEPET11 (7.5 × 104 IJs/g per larvae). In the assay in field conditions, all treatments differed from the control treatment, no significant differences were observed in the evaluated factors (isolates and insect populations), and the highest percentage of mortality was obtained by the UEPN05 isolate when applied to insects from the laboratory (18.57% mortality).

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Acknowledgements

We would like to thank EMBRAPA for providing the insects and a place to conduct the study.

Funding

This study was financed by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001, Fundação Araucária and Universidade Estadual do Norte do Paraná – UENP/PROPG/EDITORA UENP..

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Authors and Affiliations

  1. Univ Estadual de Londrina, Londrina, PR, Brazil

    Douglas Martins Cecconello

  2. Embrapa Soja, Londrina, PR, Brazil

    Samuel Roggia

  3. Univ Estadual Do Norte Do Paraná Campus Luiz Meneghel, Bandeirantes, PR, Brazil

    Gabriela Souza Doneze

  4. Instituto Biológico, Campinas, SP, Brazil

    Mariana Ferracin de Macedo

  5. Univ Estadual Do Norte Do Paraná Campus, Cornélio Procópio, PR, Brazil

    Viviane Sandra Alves

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  1. Douglas Martins Cecconello
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  2. Samuel Roggia
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  3. Gabriela Souza Doneze
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  4. Mariana Ferracin de Macedo
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  5. Viviane Sandra Alves
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Douglas Martins Cecconello, Samuel Roggia, Gabriela Souza Doneze, Mariana Ferracin de Macedo e Viviane Sandra Alves. The first draft of the manuscript was written by Douglas Martins Cecconello and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Viviane Sandra Alves.

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Cecconello, D.M., Roggia, S., Doneze, G.S. et al. Heterorhabditis amazonensis to Control Euschistus heros (Hemiptera: Pentatomidae) in Laboratory and Field Conditions. Neotrop Entomol 51, 292–298 (2022). https://doi.org/10.1007/s13744-021-00936-5

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