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Biosynthesized Gold Nanoparticles Integrated Ointment Base for Repellent Activity Against Aedes aegypti L

  • Medical and Veterinary Entomology
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Abstract

The present study focused on preparing a nano-ointment base integrated with biogenic gold nanoparticles from Artemisia vulgaris L. leaf extract. As prepared, nano-ointment was characterized by using Fourier-transform infrared spectroscopy, and the morphology of the nano-ointment was confirmed through a scanning electron microscope. Initially, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide results showed nano-ointment cytocompatibility at different concentrations (20–200 μg/mL) against L929 cells. The in vitro hemolysis assay also revealed that the nano-ointment is biocompatible. Further studies confirmed that nano-ointment has repellent activity with various concentrations (12.5, 25, 50, 75, and 100 ppm). At 100 ppm concentration, the highest repellent activity was observed at 60-min protection time against the Aedes aegypti L. female mosquitoes. The results indicated that the increasing concentration of nano-ointment prolongs the protection time. Moreover, the outcome of this study provides an alternative nano-ointment to synthetic repellent and insecticides after successful clinical trials. It could be an eco-friendly, safer nano-bio repellent, which can protect from dengue fever mosquitoes.

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Authors and Affiliations

  1. College of Horticulture and Landscape Architecture, Southwest University, Chongqing, People’s Republic of China

    Balasubramani Sundararajan & Zhiqin Zhou

  2. School of Materials and Energy, Southwest University, Chongqing, People’s Republic of China

    Gnanasekar Sathishkumar

  3. Dept of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

    Prabu kumar Seetharaman & Sivaperumal Sivaramakrishnan

  4. Dept of Biotechnology, Aditya Degree and PG College, Kakinada, Andhra Pradesh, India

    Anil Kumar Moola

  5. Dept of Botany, Arignar Anna Govt Arts College, Musiri, Tiruchirappalli, Tamil Nadu, India

    Saravanamoorthy Mutharasanallur Duraisamy

  6. College of Pharmacy (Girls), Prince Sattam Bin Abdulaziz University, Al-Kharj, Kingdom of Saudi Arabia

    Al Anoud Saud Bin Mutayran & Vidya Devanathadesikan Seshadri

  7. School of Pharmacy, Catholic University of Health and Allied Sciences, Mwanza, Tanzania

    Adelina Thomas

  8. Dept of Botany, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

    Bollipo Diana Ranjitha Kumari

  9. Division of Livestock and Human Diseases Vector Control, Mosquito Section, Tropical Pesticides Research Institute, Arusha, Tanzania

    Eliningaya J. Kweka

  10. Dept of Medical Parasitology and Entomology, School of Medicine, Catholic University of Health and Allied Sciences, Mwanza, Tanzania

    Eliningaya J. Kweka

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  1. Balasubramani Sundararajan
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  2. Gnanasekar Sathishkumar
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  3. Prabu kumar Seetharaman
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  6. Al Anoud Saud Bin Mutayran
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  8. Adelina Thomas
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  11. Eliningaya J. Kweka
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  12. Zhiqin Zhou
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Contributions

B.S. and B.D.R.K. conceived of the idea of the work. B.S. developed the study, performed the wrote the main manuscript. B.S., G.S., P.S., and S.S. carried out the experiments. A.K.M., S.M., A.T., and E.J. analyzed data interpretation. A.A.S.B.M., V.D.S., and Z.Z. revised the manuscript contents. All authors discussed the results and contributed to the final manuscript.

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Correspondence to Balasubramani Sundararajan or Zhiqin Zhou.

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Sundararajan, B., Sathishkumar, G., Seetharaman, P.k. et al. Biosynthesized Gold Nanoparticles Integrated Ointment Base for Repellent Activity Against Aedes aegypti L. Neotrop Entomol 51, 151–159 (2022). https://doi.org/10.1007/s13744-021-00920-z

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