Antennal Morphology and Localization of a Pheromone-Binding Protein of Lobesia botrana (Denis & Schiffermüller) (Lepidoptera: Tortricidae)

Abstract

In the sensory system of insects, olfactory sensilla constitute important functional elements for discriminating odors. Therefore, we used light microscopy and scanning electron microscopy to investigate the morphology and distribution of sensilla in the antennae of Lobesia botrana (Denis & Schiffermüller). In addition, we studied the expression of the gene encoding for pheromone-binding protein 1 (LbotPBP1) by in situ hybridization. Lobesia botrana antennae are filiform and are subdivided into three segments: scape, pedicel, and flagellum. The number of flagellum and their overall length were significantly higher and longer in males than in females. Six morphological types of sensilla (trichodea, chaetica, coeloconica, auricillica, basiconica, and styloconica) were identified on the antennae of both sexes. Trichodea sensilla were the most abundant on the antennae of L. botrana, and three subtypes, discerned by their lengths, were observed. However, sensilla trichodea subtype III was only present in male antennae. Moreover, LbotPBP1 expression was restricted to this type of sensilla, thus confirming its olfactory role, specifically under the context of sexual pheromone perception.

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Acknowledgments

This study was supported by FONDECYT project 11140668.

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AM, AQ, and RP planned, designed, and executed experimental work. PL, CA, FM, and HV executed experimental work with antennae. RG and AM performed in situ hybridization. MP, LB, and LM performed data analyses. AM, RG, and HV wrote the manuscript.

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Correspondence to A. Mutis.

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Edited by Roberto Romani – Univ of Perugia

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Godoy, R., Aburto, C., Lizana, P. et al. Antennal Morphology and Localization of a Pheromone-Binding Protein of Lobesia botrana (Denis & Schiffermüller) (Lepidoptera: Tortricidae). Neotrop Entomol 48, 422–432 (2019). https://doi.org/10.1007/s13744-018-0648-x

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Keywords

  • Sensilla
  • scanning electron microscopy
  • in situ hybridization
  • pheromone-binding protein