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Neotropical Entomology

, Volume 48, Issue 3, pp 422–432 | Cite as

Antennal Morphology and Localization of a Pheromone-Binding Protein of Lobesia botrana (Denis & Schiffermüller) (Lepidoptera: Tortricidae)

  • R. Godoy
  • C. Aburto
  • P. Lizana
  • H. Venthur
  • R. Palma-Millanao
  • L. Méndez
  • M. Panichini
  • F. Moraga
  • L. Bardehle
  • A. Quiroz
  • A. MutisEmail author
Systematics, Morphology and Physiology
  • 145 Downloads

Abstract

In the sensory system of insects, olfactory sensilla constitute important functional elements for discriminating odors. Therefore, we used light microscopy and scanning electron microscopy to investigate the morphology and distribution of sensilla in the antennae of Lobesia botrana (Denis & Schiffermüller). In addition, we studied the expression of the gene encoding for pheromone-binding protein 1 (LbotPBP1) by in situ hybridization. Lobesia botrana antennae are filiform and are subdivided into three segments: scape, pedicel, and flagellum. The number of flagellum and their overall length were significantly higher and longer in males than in females. Six morphological types of sensilla (trichodea, chaetica, coeloconica, auricillica, basiconica, and styloconica) were identified on the antennae of both sexes. Trichodea sensilla were the most abundant on the antennae of L. botrana, and three subtypes, discerned by their lengths, were observed. However, sensilla trichodea subtype III was only present in male antennae. Moreover, LbotPBP1 expression was restricted to this type of sensilla, thus confirming its olfactory role, specifically under the context of sexual pheromone perception.

Keywords

Sensilla scanning electron microscopy in situ hybridization pheromone-binding protein 

Notes

Acknowledgments

This study was supported by FONDECYT project 11140668.

Author’s Contributions

AM, AQ, and RP planned, designed, and executed experimental work. PL, CA, FM, and HV executed experimental work with antennae. RG and AM performed in situ hybridization. MP, LB, and LM performed data analyses. AM, RG, and HV wrote the manuscript.

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Copyright information

© Sociedade Entomológica do Brasil 2018

Authors and Affiliations

  • R. Godoy
    • 1
    • 2
  • C. Aburto
    • 1
  • P. Lizana
    • 1
  • H. Venthur
    • 2
    • 3
  • R. Palma-Millanao
    • 4
  • L. Méndez
    • 2
  • M. Panichini
    • 5
  • F. Moraga
    • 6
  • L. Bardehle
    • 2
    • 3
    • 7
  • A. Quiroz
    • 2
    • 3
  • A. Mutis
    • 2
    • 3
    Email author
  1. 1.Carrera de Bioquímica, Departamento de Ciencias Químicas y Recursos NaturalesUniversidad de La FronteraTemucoChile
  2. 2.Centro de Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA)Universidad de La FronteraTemucoChile
  3. 3.Laboratorio de Química Ecológica, Departamento de Ciencias Químicas y Recursos NaturalesUniversidad de La FronteraTemucoChile
  4. 4.Instituto de Ciencias Biológicas, Millennium Nucleus Centre in Molecular Ecology and Evolutionary Applications in the AgroecosystemsUniversidad de TalcaTalcaChile
  5. 5.Instituto de Investigaciones Agropecuarias (INIA Quilamapu)ChillánChile
  6. 6.Doctorado en Ciencias de Recursos Naturales, Facultad de Ingeniería y CienciasUniversidad de La FronteraTemucoChile
  7. 7.Scientific and Technological Bioresource Nucleus (BIOREN-UFRO)Universidad de La FronteraTemucoChile

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