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Phylogeography Approach of Diloboderus abderus (Coleoptera: Melolonthidae) in the Southern Cone of America

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Abstract

Diloboderus abderus (Sturm, 1826) (Coleoptera: Melolonthidae) is a serious soil pest of corn, wheat, oat, and natural and cultivated pastures in Argentina, Paraguay, Uruguay, and southern Brazil. Despite its economic importance, the genetic diversity and population structure of D. abderus remain unknown. We sequenced a fragment of the mitochondrial gene cytochrome oxidase I region (COI), of six populations of D. abderus from the Southern Cone of America. The mtDNA marker revealed a high haplotype diversity, high pairwise FST values, and significant genetic variations among populations. No correlation was found between genetic and geographical distances, yet the most common haplotype (Dab01) was present in four out of the six populations. Analysis of molecular variance showed that most of the variation was within populations of D. abderus. Tajima’s D and Fu’s FS tests indicated no evidence that D. abderus populations are under recent expansion. Our results indicate that genetic-based traits will likely remain localized or spread slowly, and management strategies need to be undertaken on a small scale.

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Acknowledgments

We thank Isac Aires de Castro, Alberto Rohrig, Eduardo Bassan Bortoluzi, Luis Eduardo Curioletti, Andrés A. Risso, and Enrique Castiglioni for assisting with the sample collection. We also thank Ashley Dean for revising the English and CAPES for granting a scholarship to the first author. CBB was co-financed by the Brazilian Government (CAPES scholar, proceeding PNPD20132610 – 42002010008P0–UFSM/Engenharia Agrícola).

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Authors and Affiliations

  1. Dept of Crop Protection, Federal Univ of Santa Maria, Santa Maria, Rio Grande do Sul, Brasil

    I Valmorbida, J A Arnemann, C B Bevilacqua, C R Perini, G A Ugalde & J V C Guedes

  2. Dept of Zoology, Federal Univ of Paraná, Curitiba, PR, Brasil

    M A Cherman

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  1. I Valmorbida
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  2. J A Arnemann
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  3. M A Cherman
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  4. C B Bevilacqua
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  5. C R Perini
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  6. G A Ugalde
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  7. J V C Guedes
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Contributions

IV, JAA, and JVCG conceived and designed the analysis; IV, GAU, and CRP executed the experimental work; IV, JAA, and CBB performed the analysis; IV, JAA, MAC, and CRP drafted the manuscript; IV, JAA, JVCG, and MAC critically revised the manuscript.

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Correspondence to I Valmorbida.

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Edited by Alberto S Corrêa – ESALQ/USP

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Valmorbida, I., Arnemann, J.A., Cherman, M.A. et al. Phylogeography Approach of Diloboderus abderus (Coleoptera: Melolonthidae) in the Southern Cone of America. Neotrop Entomol 48, 332–339 (2019). https://doi.org/10.1007/s13744-018-0637-0

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