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Identification and Expression Profile of Two Putative Odorant-Binding Proteins from the Neotropical Brown Stink Bug, Euschistus heros (Fabricius) (Hemiptera: Pentatomidae)

Neotropical Entomology volume 43, pages 106–114 (2014)Cite this article

Abstract

This work presents the first identification of putative odorant-binding proteins (OBPs) from a member of the Pentatomidae, i.e. the brown stink bug Euschistus heros (Fabricius), an important pest of soybean in Brazil. Antennae from both sexes of E. heros adults (12 days old and unmated) were used to construct a cDNA library, from which two transcripts encoding putative E. heros OBPs (EherOBPs) were identified. The expression levels of EherOBP1 and EherOBP2 were found to be higher in male antennae than in female and there was difference in expression in legs, wings, and abdomens of the two sexes. The histolocalization of EherOBP1 and EherOBP2 transcripts in antennae also showed a sexual dimorphism in the chemoreception system, with different expression sites in the antennal segments between males and females, occurring predominantly at the base of the sensillum. The implications of these findings for stink bug chemoreception are discussed.

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Acknowledgements

We thank Hélio Moreira dos Santos and Diva Tibúrcio for rearing the insects, Diva Dusi for support in the ISH methodology, Maurício Machaim for authorization to use the real time qPCR machine and Renata Velozo Timbó for technical assistance, all from the Biological Control unit of the Brazilian Agricultural Research Corporation (Embrapa) Genetic Resources and Biotechnology Center (Cenargen). This research was supported by grants from Fundação de Apoio à Pesquisa do Distrito Federal (FAP-DF) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council (BBSRC) of the UK.

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Affiliations

  1. Instituto de Ciências Biológicas, Univ de Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF, Brasil

    L R Farias & S N Báo

  2. Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brasil

    L R Farias, D P Paula, G J Pappas Jr., M C B Moraes, R A Laumann & M Borges

  3. Dept of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, UK

    J J Zhou, R Liu, M A Birkett, J A Pickett & L M Field

Authors
  1. L R Farias
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  2. D P Paula
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  3. J J Zhou
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  4. R Liu
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  5. G J Pappas Jr.
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  6. M C B Moraes
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  7. R A Laumann
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  8. M Borges
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  9. M A Birkett
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  10. J A Pickett
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  11. L M Field
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  12. S N Báo
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Corresponding author

Correspondence to L R Farias.

Additional information

Edited by Stefano Colazza – University of Palermo

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Online Resource 1

Percentage distribution of putative protein families found in the antenna of the neotropical stink bug, Euschistus heros. (DOC 31 kb)

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Farias, L.R., Paula, D.P., Zhou, J.J. et al. Identification and Expression Profile of Two Putative Odorant-Binding Proteins from the Neotropical Brown Stink Bug, Euschistus heros (Fabricius) (Hemiptera: Pentatomidae). Neotrop Entomol 43, 106–114 (2014). https://doi.org/10.1007/s13744-013-0187-4

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  • DOI: https://doi.org/10.1007/s13744-013-0187-4

Keywords

  • Antennae
  • amphipathic proteins
  • chemoreception
  • olfaction
  • semiochemicals
  • sensillum