Skip to main content
SpringerLink
Log in

A signal amplification by QDs used for ferrocene-labeled sandwich aptasensor for determination of Hg2+ in water samples

  • Original Paper
  • Published:
Journal of the Iranian Chemical Society Aims and scope Submit manuscript

Abstract

In this paper, the design of a novel sandwich-type electrochemical aptasensor was reported for an ultrasensitive mercury ion (Hg2+) detection in water samples, which labeled with two-labeled aptamer (Apt) sequences. The used Apts were Apt1 and Apt2 as the capture and signal probe, respectively. The Apt1 probe was immobilized on the poly(4-aminobenzoic acid) (p-ABA) and quantum dots (QDs) film as the platform, as well as the Apt2 reporter was labeled with ferrocene. In the presence of Hg2+, the strong coordination complex has been formed between the specific thymine of the Apt1, Hg2+, as well as the thymine of the Apt as T–Hg2+–T adduct. The QDs and p-ABA were applied for increasing the conductivity of platform and suitable binding of the recognition elements. Under the optimized conditions, the constructed aptasensor illustrated either a wide linear relationship between the logarithm of Hg2+ concentration and current, from 0.05 to 100 nM and also an excellent low limit of detection of 0.01 nM. The quality of carefully choosing, an excellent stability and specificity sensitivity of the designed aptasensor, was investigated by spiked tap water samples as real sample. Moreover, the aptasensor exhibits the good reproducibility as well as has high selectivity for the other cations. The recoveries of the Hg2+ assay of the tap water samples were acquired satisfactorily which imply the generated aptasensor can use Hg2+ measurement in the real laboratories.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. K.J. Powell, P.L. Brown, R.H. Byrne, T. Gajda, G. Hefter, S. Sjöberg, H. Wanner, Pure Appl. Chem. 77, 739 (2005)

    CAS  Google Scholar 

  2. S. Amiri, R. Ahmadi, A. Salimi, A. Navaee, S.H. Qaddare, M.K. Amini, New J. Chem. 42, 16027 (2018)

    CAS  Google Scholar 

  3. P. Miao, L. Liu, Y. Li, G. Li, Electrochem. Commun. 11, 1904 (2009)

    CAS  Google Scholar 

  4. L. Hong, H. Xian-Deng, L. Zhou, Chin. J. Anal. Chem. 43, 1291 (2015)

    Google Scholar 

  5. P. Makam, R. Shilpa, A.E. Kandjani, S.R. Periasamy, Y.M. Sabri, C. Madhu, S.K. Bhargava, T. Govindaraju, Biosens. Bioelectron. 100, 556 (2018)

    CAS  Google Scholar 

  6. Y. Liu, X. Wang, H. Wu, Biosens. Bioelectron. 87, 129 (2017)

    CAS  Google Scholar 

  7. H. Guo, J. Li, Y. Li, D. Wu, H. Ma, Q. Wei, B. Du, New J. Chem. 42, 11147 (2018)

    CAS  Google Scholar 

  8. S.K. Kim, M. Gupta, H.-I. Lee, Sens. Actuators B Chem. 257, 728 (2018)

    CAS  Google Scholar 

  9. V. Amani, R. Alizadeh, H.S. Alavije, S.F. Heydari, M. Abafat, J. Mol. Struct. 1142, 92 (2017)

    CAS  Google Scholar 

  10. F. Domanico, G. Forte, C. Majorani, O. Senofonte, F. Petrucci, V. Pezzi, A. Alimonti, J. Trace Elem. Med. Biol. 43, 3 (2017)

    CAS  PubMed  Google Scholar 

  11. M.R.R. Kahkha, S. Daliran, A.R. Oveisi, M. Kaykhaii, Z. Sepehri, Food Anal. Methods 10, 2175 (2017)

    Google Scholar 

  12. J.-L. Chen, P.-C. Yang, T. Wu, Y.-W. Lin, Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 199, 301 (2018)

    CAS  Google Scholar 

  13. Y. Yamini, M. Safari, Microchem. J. 143, 503 (2018)

    CAS  Google Scholar 

  14. C. Gao, Q. Wang, F. Gao, F. Gao, Chem. Commun. 50, 9397 (2014)

    CAS  Google Scholar 

  15. J. Tu, Y. Gan, T. Liang, Q. Hu, Q. Wang, T. Ren, Q. Sun, H. Wan, P. Wang, Front. Chem. 6, 333 (2018)

    PubMed  PubMed Central  Google Scholar 

  16. X. Song, B. Fu, Y. Lan, Y. Chen, Y. Wei, C. Dong, Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 204, 301 (2018)

    CAS  Google Scholar 

  17. S.K. Daniel, A. Kumar, K. Sivasakthi, C.S. Thakur, Sens. Actuators B Chem. 290, 73 (2019)

    Google Scholar 

  18. C. Rullyani, M. Shellaiah, M. Ramesh, H.-C. Lin, C.-W. Chu, Org. Electron. 69, 275 (2019)

    CAS  Google Scholar 

  19. Q.-L. Wang, H.-F. Cui, X. Song, S.-F. Fan, L.-L. Chen, M.-M. Li, Z.-Y. Li, Sens. Actuators B Chem. 260, 48 (2018)

    CAS  Google Scholar 

  20. Y. Zhang, J. Xia, F. Zhang, Z. Wang, Q. Liu, Sens. Actuators B Chem. 267, 412 (2018)

    CAS  Google Scholar 

  21. S. Liu. Y. Wang. W. Xu. X. Leng. H. Wang. Y. Guo, J. Huang, Biosensors and Bioelectronics 88 (2017) 181

    CAS  PubMed  Google Scholar 

  22. G. Shen, X. Zhang, S. Zhang, J. Electrochem. Soc. 161, B256 (2014)

    Google Scholar 

  23. H.B. Seo, M.B. Gu, J. Biol. Eng. 11, 11 (2017)

    PubMed  PubMed Central  Google Scholar 

  24. M.C. Rodriguez, A.-N. Kawde, J. Wang, Chem. Commun. 34, 4267 (2005)

    Google Scholar 

  25. A. Abi, Z. Mohammadpour, X. Zuo, A. Safavi, Biosens. Bioelectron. 102, 479 (2018)

    CAS  PubMed  Google Scholar 

  26. D. Li, A. Wieckowska, I. Willner, Angew. Chem. Int. Ed. 47, 3927 (2008)

    CAS  Google Scholar 

  27. C.H. Chung, J.H. Kim, J. Jung, B.H. Chung, Biosens. Bioelectron. 41, 827 (2013)

    CAS  PubMed  Google Scholar 

  28. E. Xiong, L. Wu, J. Zhou, P. Yu, X. Zhang, J. Chen, Anal. Chim. Acta 853, 242 (2015)

    CAS  PubMed  Google Scholar 

  29. C. Tortolini, P. Bollella, M.L. Antonelli, R. Antiochia, F. Mazzei, G. Favero, Biosens. Bioelectron. 67, 524 (2015)

    CAS  PubMed  Google Scholar 

  30. D. Han, Y.-R. Kim, J.-W. Oh, T.H. Kim, R.K. Mahajan, J.S. Kim, H. Kim, Analyst 134, 1857 (2009)

    CAS  PubMed  Google Scholar 

  31. B. Seiwert, U. Karst, Anal. Bioanal. Chem. 390, 181 (2008)

    CAS  PubMed  Google Scholar 

  32. A. Anne, A. Bouchardon, J. Moiroux, J. Am. Chem. Soc. 125, 1112 (2003)

    CAS  PubMed  Google Scholar 

  33. S. Yu, Y. Tang, Z. Li, K. Chen, X. Ding, B. Yu, Photonics Res. 6, 90 (2018)

    CAS  Google Scholar 

  34. H. Huang, J.-J. Zhu, Analyst 138, 5855 (2013)

    CAS  PubMed  Google Scholar 

  35. O. Goryacheva, P. Mishra, Y.I. Goryacheva, Talanta 179, 456 (2017)

    PubMed  Google Scholar 

  36. M. Pedrero, S. Campuzano, J.M. Pingarrón, J. AOAC Int. 100, 950 (2017)

    CAS  PubMed  Google Scholar 

  37. R. Bala, A. Swami, I. Tabujew, K. Peneva, N. Wangoo, R.K. Sharma, Biosens. Bioelectron. 104, 45 (2017)

    PubMed  Google Scholar 

  38. Y.-P. Wei, X.-P. Liu, C.-J. Mao, H.-L. Niu, J.-M. Song, B.-K. Jin, Biosens. Bioelectron. 103, 99 (2018)

    CAS  PubMed  Google Scholar 

  39. A. Sun, J. Chai, T. Xiao, X. Shi, X. Li, Q. Zhao, D. Li, J. Chen, Sens. Actuators B Chem. 258, 408 (2018)

    CAS  Google Scholar 

  40. H.N. Abdelhamid, H.-F. Wu, Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 188, 50 (2018)

    CAS  Google Scholar 

  41. H. Kim, J.D. Song, Appl. Surf. Sci. 427, 405 (2018)

    CAS  Google Scholar 

  42. X. Cui, V.A. Lee, Y. Raphael, J.A. Wiler, J.F. Hetke, D.J. Anderson, D.C. Martin, J. Biomed. Mater. Res. Part A 56, 261 (2001)

    CAS  Google Scholar 

  43. P.D. Tran, A. Le Goff, J. Heidkamp, B. Jousselme, N. Guillet, S. Palacin, H. Dau, M. Fontecave, V. Artero, Angew. Chem. 123, 1407 (2011)

    Google Scholar 

  44. T. Kilic, A. Erdem, Y. Erac, M.O. Seydibeyoglu, S. Okur, M. Ozsoz, Electroanalysis 27, 317 (2015)

    CAS  Google Scholar 

  45. P. Lee, K. Ward, K. Tschulik, G. Chapman, R. Compton, Electroanalysis 26, 366 (2014)

    CAS  Google Scholar 

  46. S. Sharma, N. Singh, V. Tomar, R. Chandra, Biosens. Bioelectron. 107, 76 (2018)

    CAS  PubMed  Google Scholar 

  47. D.R. Kumar, S. Kesavan, M.L. Baynosa, J.-J. Shim, Electrochim. Acta 246, 1131 (2017)

    CAS  Google Scholar 

  48. M. Safari, S. Najafi, E. Arkan, S. Amani, M. Shahlaei, Microchem. J. 146, 293 (2019)

    CAS  Google Scholar 

  49. P. Dai, G. Zhang, Y. Chen, H. Jiang, Z. Feng, Z. Lin, J. Zhan, Chem. Commun. 48, 3006 (2012)

    CAS  Google Scholar 

  50. Y. Liu, J. Lei, Y. Huang, H. Ju, Anal. Chem. 86, 8735 (2014)

    CAS  PubMed  Google Scholar 

  51. M. Shamsipur, A. Pashabadi, F. Molaabasi, S. Hosseinkhani, Biosens. Bioelectron. 90, 195 (2017)

    CAS  PubMed  Google Scholar 

  52. X. Li, X. Niu, W. Zhao, W. Chen, C. Yin, Y. Men, G. Li, W. Sun, Electrochem. Commun. 86, 68 (2018)

    CAS  Google Scholar 

  53. W. Chen, W. Weng, C. Yin, X. Niu, G. Li, H. Xie, J. Liu, W. Sun, Int. J. Electrochem. Sci 13, 4741 (2018)

    CAS  Google Scholar 

  54. S.A. Frank, F1000Research 7, 200 (2018)

    PubMed  PubMed Central  Google Scholar 

  55. H. Ehzari, M. Safari, M. Shahlaei, Microchem. J. 143, 118 (2018)

    CAS  Google Scholar 

  56. X. Zhu, L. Chen, Z. Lin, B. Qiu, G. Chen, Chem. Commun. 46, 3149 (2010)

    CAS  Google Scholar 

  57. J. Wan, G. Yin, X. Ma, L. Xing, X. Luo, Electroanalysis 26, 823 (2014)

    CAS  Google Scholar 

  58. H. Park, S.-J. Hwang, K. Kim, Electrochem. Commun. 24, 100 (2012)

    CAS  Google Scholar 

  59. N. Zhou, J. Li, H. Chen, C. Liao, L. Chen, Analyst 138, 1091 (2013)

    CAS  PubMed  Google Scholar 

  60. J. Tang, Y. Huang, C. Zhang, H. Liu, D. Tang, Microchim. Acta 183, 1805 (2016)

    CAS  Google Scholar 

  61. J. Chen, J. Tang, J. Zhou, L. Zhang, G. Chen, D. Tang, Anal. Chim. Acta 810, 10 (2014)

    CAS  PubMed  Google Scholar 

  62. Z. Lin, X. Li, H.-B. Kraatz, Anal. Chem. 83, 6896 (2011)

    CAS  PubMed  Google Scholar 

  63. S. Tang, P. Tong, W. Lu, J. Chen, Z. Yan, L. Zhang, Biosens. Bioelectron. 59, 1 (2014)

    CAS  PubMed  Google Scholar 

  64. J. Li, M. Sun, X. Wei, L. Zhang, Y. Zhang, Biosens. Bioelectron. 74, 423 (2015)

    CAS  PubMed  Google Scholar 

  65. J. Chen, S. Zhou, J. Wen, Anal. Chem. 86, 3108 (2014)

    CAS  PubMed  Google Scholar 

  66. S.-H. Wu, B. Zhang, F.-F. Wang, Z.-Z. Mi, J.-J. Sun, Biosens. Bioelectron. 104, 145 (2018)

    CAS  PubMed  Google Scholar 

  67. Y.L. Huang, Z.F. Gao, J. Jia, H.Q. Luo, N.B. Li, J. Hazard. Mater. 308, 173 (2016)

    CAS  PubMed  Google Scholar 

  68. R.-F. Huang, H.-X. Liu, Q.-Q. Gai, G.-J. Liu, Z. Wei, Biosens. Bioelectron. 71, 194 (2015)

    CAS  PubMed  Google Scholar 

  69. T. Bao, W. Wen, L. Shu, X. Zhang, S. Wang, New J. Chem. 40, 6686 (2016)

    CAS  Google Scholar 

  70. S. Amiri, A. Navaee, A. Salimi, R. Ahmadi, Electrochem. Commun. 78, 21 (2017)

    CAS  Google Scholar 

  71. M. Lu, R. Xiao, X. Zhang, J. Niu, X. Zhang, Y. Wang, Biosens. Bioelectron. 85, 267 (2016)

    CAS  PubMed  Google Scholar 

  72. B. Babamiri, A. Salimi, R. Hallaj, Biosens. Bioelectron. 102, 328 (2018)

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Hosna Ehzari

  2. Department of Chemical Engineering, Kermanshah University of Technology, Kermanshah, Iran

    Meysam Safari

  3. Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mohsen Shahlaei

Authors
  1. Hosna Ehzari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Meysam Safari
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mohsen Shahlaei
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohsen Shahlaei.

Electronic supplementary material

Below is the link to the electronic supplementary material.

TEM image of CdTe (DOCX 128 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ehzari, H., Safari, M. & Shahlaei, M. A signal amplification by QDs used for ferrocene-labeled sandwich aptasensor for determination of Hg2+ in water samples. J IRAN CHEM SOC 16, 2555–2564 (2019). https://doi.org/10.1007/s13738-019-01718-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13738-019-01718-y

Keywords

Search

Navigation